Nuclear Magnetic Resonance Fingerprinting and Principal Component Analysis Strategies Lead to Anti-Tuberculosis Natural Product Discovery from Actinomycetes
Background: The increasing prevalence of drug-resistant tuberculosis (TB) underscores the urgent need for novel antimicrobial agents. Methods: This study integrates cultivation optimization, nuclear magnetic resonance (NMR) fingerprinting, and principal component analysis (PCA) to explore microbial...
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| Format: | Article |
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MDPI AG
2025-01-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/14/1/108 |
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| author | Jianying Han Xueting Liu Lixin Zhang Ronald J. Quinn Miaomiao Liu |
| author_facet | Jianying Han Xueting Liu Lixin Zhang Ronald J. Quinn Miaomiao Liu |
| author_sort | Jianying Han |
| collection | DOAJ |
| description | Background: The increasing prevalence of drug-resistant tuberculosis (TB) underscores the urgent need for novel antimicrobial agents. Methods: This study integrates cultivation optimization, nuclear magnetic resonance (NMR) fingerprinting, and principal component analysis (PCA) to explore microbial secondary metabolites as potential anti-TB agents. Results: Using the combined approach, 11 bioactive compounds were isolated and identified, all exhibiting anti-<i>Mycobacterium bovis</i> BCG activity. Notable findings include borrelidin, a potent threonyl-tRNA synthetase inhibitor with broad biological activities, and L-O-Lac-L-Val-D-O-Hiv-D-Val, a peptide isolated for the first time from a plant endophyte, demonstrating broad-spectrum antimicrobial activity. Additionally, elaiophylin and polycyclic tetramate macrolactams (PTMs) displayed significant bactericidal effects, with elaiophylin achieving complete BCG inhibition at 72 h and PTMs marking their first reported anti-TB activity. The study also identified bafilomycins as potent scaffolds for anti-TB drug development, showcasing rapid bactericidal activity at low MIC values. Conclusions: These findings emphasize the value of microbial metabolites as a reservoir of bioactive compounds and provide new avenues for developing next-generation anti-TB therapies. |
| format | Article |
| id | doaj-art-6c2bc98b532247d18ac58c38349195ee |
| institution | Kabale University |
| issn | 2079-6382 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj-art-6c2bc98b532247d18ac58c38349195ee2025-01-24T13:19:03ZengMDPI AGAntibiotics2079-63822025-01-0114110810.3390/antibiotics14010108Nuclear Magnetic Resonance Fingerprinting and Principal Component Analysis Strategies Lead to Anti-Tuberculosis Natural Product Discovery from ActinomycetesJianying Han0Xueting Liu1Lixin Zhang2Ronald J. Quinn3Miaomiao Liu4Institute for Biomedicine and Glycomics, Griffith University, Brisbane, QLD 4111, AustraliaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, ChinaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, ChinaInstitute for Biomedicine and Glycomics, Griffith University, Brisbane, QLD 4111, AustraliaInstitute for Biomedicine and Glycomics, Griffith University, Brisbane, QLD 4111, AustraliaBackground: The increasing prevalence of drug-resistant tuberculosis (TB) underscores the urgent need for novel antimicrobial agents. Methods: This study integrates cultivation optimization, nuclear magnetic resonance (NMR) fingerprinting, and principal component analysis (PCA) to explore microbial secondary metabolites as potential anti-TB agents. Results: Using the combined approach, 11 bioactive compounds were isolated and identified, all exhibiting anti-<i>Mycobacterium bovis</i> BCG activity. Notable findings include borrelidin, a potent threonyl-tRNA synthetase inhibitor with broad biological activities, and L-O-Lac-L-Val-D-O-Hiv-D-Val, a peptide isolated for the first time from a plant endophyte, demonstrating broad-spectrum antimicrobial activity. Additionally, elaiophylin and polycyclic tetramate macrolactams (PTMs) displayed significant bactericidal effects, with elaiophylin achieving complete BCG inhibition at 72 h and PTMs marking their first reported anti-TB activity. The study also identified bafilomycins as potent scaffolds for anti-TB drug development, showcasing rapid bactericidal activity at low MIC values. Conclusions: These findings emphasize the value of microbial metabolites as a reservoir of bioactive compounds and provide new avenues for developing next-generation anti-TB therapies.https://www.mdpi.com/2079-6382/14/1/108tuberculosisactinomycetesborrelidinelaiophylinpolycyclic tetramate macrolactamsbafilomycin |
| spellingShingle | Jianying Han Xueting Liu Lixin Zhang Ronald J. Quinn Miaomiao Liu Nuclear Magnetic Resonance Fingerprinting and Principal Component Analysis Strategies Lead to Anti-Tuberculosis Natural Product Discovery from Actinomycetes Antibiotics tuberculosis actinomycetes borrelidin elaiophylin polycyclic tetramate macrolactams bafilomycin |
| title | Nuclear Magnetic Resonance Fingerprinting and Principal Component Analysis Strategies Lead to Anti-Tuberculosis Natural Product Discovery from Actinomycetes |
| title_full | Nuclear Magnetic Resonance Fingerprinting and Principal Component Analysis Strategies Lead to Anti-Tuberculosis Natural Product Discovery from Actinomycetes |
| title_fullStr | Nuclear Magnetic Resonance Fingerprinting and Principal Component Analysis Strategies Lead to Anti-Tuberculosis Natural Product Discovery from Actinomycetes |
| title_full_unstemmed | Nuclear Magnetic Resonance Fingerprinting and Principal Component Analysis Strategies Lead to Anti-Tuberculosis Natural Product Discovery from Actinomycetes |
| title_short | Nuclear Magnetic Resonance Fingerprinting and Principal Component Analysis Strategies Lead to Anti-Tuberculosis Natural Product Discovery from Actinomycetes |
| title_sort | nuclear magnetic resonance fingerprinting and principal component analysis strategies lead to anti tuberculosis natural product discovery from actinomycetes |
| topic | tuberculosis actinomycetes borrelidin elaiophylin polycyclic tetramate macrolactams bafilomycin |
| url | https://www.mdpi.com/2079-6382/14/1/108 |
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