Unveiling BID: a key biomarker in apoptosis post-intracerebral hemorrhage
BackgroundApoptosis plays a significant role in secondary brain injury following intracerebral hemorrhage (ICH). Currently, the mechanisms related to cell apoptosis after cerebral hemorrhage are still under investigation.MethodsWe identified differentially expressed genes (DEGs) between human ICH pa...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Neurology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fneur.2025.1533558/full |
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| author | Kun Dai Hong-Rong Zhang Shuai-Yu Ren Ming-Pei Zhao Ming-Pei Zhao Neng Wang Neng Wang Hong-Zhi Gao Hong-Zhi Gao De-Zhi Kang De-Zhi Kang De-Zhi Kang Zong-Qing Zheng Zong-Qing Zheng Zong-Qing Zheng Zong-Qing Zheng Zong-Qing Zheng |
| author_facet | Kun Dai Hong-Rong Zhang Shuai-Yu Ren Ming-Pei Zhao Ming-Pei Zhao Neng Wang Neng Wang Hong-Zhi Gao Hong-Zhi Gao De-Zhi Kang De-Zhi Kang De-Zhi Kang Zong-Qing Zheng Zong-Qing Zheng Zong-Qing Zheng Zong-Qing Zheng Zong-Qing Zheng |
| author_sort | Kun Dai |
| collection | DOAJ |
| description | BackgroundApoptosis plays a significant role in secondary brain injury following intracerebral hemorrhage (ICH). Currently, the mechanisms related to cell apoptosis after cerebral hemorrhage are still under investigation.MethodsWe identified differentially expressed genes (DEGs) between human ICH patients and normal individuals from the GEO database and conducted GO and KEGG functional enrichment analyses on these DEGs. We then constructed a PPI network and used the MECDE algorithm to identify key genes potentially involved in apoptosis after ICH. Additionally, we identified miRNAs that might regulate apoptotic genes in an mRNA-miRNA interaction network. Finally, we validated the bioinformatics results in a rat ICH model.ResultsIn the human ICH model, 645 DEGs were identified. GO and KEGG analyses indicated that these DEGs are primarily involved in immune response, inflammatory response, and apoptosis. GSEA analysis showed significant enrichment of DEGs in the apoptotic process. By comparing with apoptosis-related genes in the MSigDB database, we identified 110 apoptosis-related genes among the 645 DEGs. Further PPI and MOCDE analyses of these apoptosis-related genes revealed that BID might be a key gene involved in apoptosis after ICH, which was validated within the rat model of ICH. The mRNA-miRNA interactions network construction suggested that miR1225-3p may be an important miRNA involved in regulating BID expression after ICH.ConclusionBID plays a critical role in the regulation of apoptosis following intracerebral hemorrhage and serves as a key biomarker in the apoptotic process after hemorrhage. |
| format | Article |
| id | doaj-art-6c248eaf64e54ecd87f97c96fbb2c7de |
| institution | DOAJ |
| issn | 1664-2295 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Neurology |
| spelling | doaj-art-6c248eaf64e54ecd87f97c96fbb2c7de2025-08-20T02:55:07ZengFrontiers Media S.A.Frontiers in Neurology1664-22952025-02-011610.3389/fneur.2025.15335581533558Unveiling BID: a key biomarker in apoptosis post-intracerebral hemorrhageKun Dai0Hong-Rong Zhang1Shuai-Yu Ren2Ming-Pei Zhao3Ming-Pei Zhao4Neng Wang5Neng Wang6Hong-Zhi Gao7Hong-Zhi Gao8De-Zhi Kang9De-Zhi Kang10De-Zhi Kang11Zong-Qing Zheng12Zong-Qing Zheng13Zong-Qing Zheng14Zong-Qing Zheng15Zong-Qing Zheng16Department of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, ChinaDepartment of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, ChinaDepartment of Neurosurgery, The First Affiliated Hospital of Soochow University, Suzhou, ChinaDepartment of Neurosurgery, Neurosurgery Research Institute, The First Affiliated Hospital, Fujian Medical University, Fuzhou, ChinaDepartment of Neurosurgery, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, ChinaDepartment of Neurosurgery, Neurosurgery Research Institute, The First Affiliated Hospital, Fujian Medical University, Fuzhou, ChinaDepartment of Neurosurgery, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, ChinaDepartment of Neurosurgery, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, ChinaClinical Center for Molecular Diagnosis and Therapy, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, ChinaDepartment of Neurosurgery, Neurosurgery Research Institute, The First Affiliated Hospital, Fujian Medical University, Fuzhou, ChinaDepartment of Neurosurgery, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, ChinaFujian Provincial Institutes of Brain Disorders and Brain Sciences, First Affiliated Hospital, Fujian Medical University, Fuzhou, ChinaDepartment of Neurosurgery, Neurosurgery Research Institute, The First Affiliated Hospital, Fujian Medical University, Fuzhou, ChinaDepartment of Neurosurgery, National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, ChinaDepartment of Neurosurgery, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, ChinaClinical Center for Molecular Diagnosis and Therapy, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, ChinaFujian Provincial Institutes of Brain Disorders and Brain Sciences, First Affiliated Hospital, Fujian Medical University, Fuzhou, ChinaBackgroundApoptosis plays a significant role in secondary brain injury following intracerebral hemorrhage (ICH). Currently, the mechanisms related to cell apoptosis after cerebral hemorrhage are still under investigation.MethodsWe identified differentially expressed genes (DEGs) between human ICH patients and normal individuals from the GEO database and conducted GO and KEGG functional enrichment analyses on these DEGs. We then constructed a PPI network and used the MECDE algorithm to identify key genes potentially involved in apoptosis after ICH. Additionally, we identified miRNAs that might regulate apoptotic genes in an mRNA-miRNA interaction network. Finally, we validated the bioinformatics results in a rat ICH model.ResultsIn the human ICH model, 645 DEGs were identified. GO and KEGG analyses indicated that these DEGs are primarily involved in immune response, inflammatory response, and apoptosis. GSEA analysis showed significant enrichment of DEGs in the apoptotic process. By comparing with apoptosis-related genes in the MSigDB database, we identified 110 apoptosis-related genes among the 645 DEGs. Further PPI and MOCDE analyses of these apoptosis-related genes revealed that BID might be a key gene involved in apoptosis after ICH, which was validated within the rat model of ICH. The mRNA-miRNA interactions network construction suggested that miR1225-3p may be an important miRNA involved in regulating BID expression after ICH.ConclusionBID plays a critical role in the regulation of apoptosis following intracerebral hemorrhage and serves as a key biomarker in the apoptotic process after hemorrhage.https://www.frontiersin.org/articles/10.3389/fneur.2025.1533558/fullICHapoptosisbioinformatics analysismicroRNABID |
| spellingShingle | Kun Dai Hong-Rong Zhang Shuai-Yu Ren Ming-Pei Zhao Ming-Pei Zhao Neng Wang Neng Wang Hong-Zhi Gao Hong-Zhi Gao De-Zhi Kang De-Zhi Kang De-Zhi Kang Zong-Qing Zheng Zong-Qing Zheng Zong-Qing Zheng Zong-Qing Zheng Zong-Qing Zheng Unveiling BID: a key biomarker in apoptosis post-intracerebral hemorrhage Frontiers in Neurology ICH apoptosis bioinformatics analysis microRNA BID |
| title | Unveiling BID: a key biomarker in apoptosis post-intracerebral hemorrhage |
| title_full | Unveiling BID: a key biomarker in apoptosis post-intracerebral hemorrhage |
| title_fullStr | Unveiling BID: a key biomarker in apoptosis post-intracerebral hemorrhage |
| title_full_unstemmed | Unveiling BID: a key biomarker in apoptosis post-intracerebral hemorrhage |
| title_short | Unveiling BID: a key biomarker in apoptosis post-intracerebral hemorrhage |
| title_sort | unveiling bid a key biomarker in apoptosis post intracerebral hemorrhage |
| topic | ICH apoptosis bioinformatics analysis microRNA BID |
| url | https://www.frontiersin.org/articles/10.3389/fneur.2025.1533558/full |
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