Characterization of two pterocarpan glycosyltransferases in Astragalus membranaceus and their application in whole-cell biocatalysis
Pterocarpan glucosides are biologically active natural products, in which the glucosyl group plays an important role. However, specific pterocarpan glycosyltransferases have rarely been reported. In this study, two highly efficient glycosyltransferases, AmGT28 and AmGT44, from Astragalus membranaceu...
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Maximum Academic Press
2024-01-01
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| Series: | Medicinal Plant Biology |
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| Online Access: | https://www.maxapress.com/article/doi/10.48130/mpb-0024-0013 |
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| author | Bai-Han Gao Meng Zhang Kuan Chen Lin-Lin Wang Ming-Ju Yao Hong-Ye Li Ming Xiong Yu-Xi Huang Yang Zhang Quan-Li Liu Juan Guo Min Ye Xue Qiao |
| author_facet | Bai-Han Gao Meng Zhang Kuan Chen Lin-Lin Wang Ming-Ju Yao Hong-Ye Li Ming Xiong Yu-Xi Huang Yang Zhang Quan-Li Liu Juan Guo Min Ye Xue Qiao |
| author_sort | Bai-Han Gao |
| collection | DOAJ |
| description | Pterocarpan glucosides are biologically active natural products, in which the glucosyl group plays an important role. However, specific pterocarpan glycosyltransferases have rarely been reported. In this study, two highly efficient glycosyltransferases, AmGT28 and AmGT44, from Astragalus membranaceus, catalyzing the conversion of medicarpin (1) to medicarpin 3-O-glucoside (1a) were identified. They could also use other pterocarpans and isoflavonoids as substrates, showing a preference for pterocarpans over isoflavonoids. A new isoflavone 7-O-glucoside 5a was prepared through a scaled-up enzymatic reaction. Furthermore, AmGT44 was expressed in E. coli to establish a whole-cell biocatalytic system (500 μL with 0.1 mM substrate), which could glucosylate pterocarpans 1-3 with a conversion rate of up to 100% and a titer of 30-38 μg/mL within 25 h. The titer of maackiain 3-O-glucoside (2a) could reach 78.66 μg/mL in a 10-mL system. The study provides efficient catalytic tools for the biosynthesis of pterocarpan glycosides. |
| format | Article |
| id | doaj-art-6c1b69901f7c484db2bb80e91132dbb6 |
| institution | DOAJ |
| issn | 2835-6969 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Medicinal Plant Biology |
| spelling | doaj-art-6c1b69901f7c484db2bb80e91132dbb62025-08-20T03:18:23ZengMaximum Academic PressMedicinal Plant Biology2835-69692024-01-01311610.48130/mpb-0024-0013mpb-0024-0013Characterization of two pterocarpan glycosyltransferases in Astragalus membranaceus and their application in whole-cell biocatalysisBai-Han Gao0Meng Zhang1Kuan Chen2Lin-Lin Wang3Ming-Ju Yao4Hong-Ye Li5Ming Xiong6Yu-Xi Huang7Yang Zhang8Quan-Li Liu9Juan Guo10Min Ye11Xue Qiao12State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, ChinaState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, ChinaState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, ChinaState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, ChinaState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, ChinaState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, ChinaState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, ChinaState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, ChinaState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, ChinaQingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences. No. 189 Songling Road, Laoshan District, Qingdao 266101, ChinaState Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100000, ChinaState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, ChinaState Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, ChinaPterocarpan glucosides are biologically active natural products, in which the glucosyl group plays an important role. However, specific pterocarpan glycosyltransferases have rarely been reported. In this study, two highly efficient glycosyltransferases, AmGT28 and AmGT44, from Astragalus membranaceus, catalyzing the conversion of medicarpin (1) to medicarpin 3-O-glucoside (1a) were identified. They could also use other pterocarpans and isoflavonoids as substrates, showing a preference for pterocarpans over isoflavonoids. A new isoflavone 7-O-glucoside 5a was prepared through a scaled-up enzymatic reaction. Furthermore, AmGT44 was expressed in E. coli to establish a whole-cell biocatalytic system (500 μL with 0.1 mM substrate), which could glucosylate pterocarpans 1-3 with a conversion rate of up to 100% and a titer of 30-38 μg/mL within 25 h. The titer of maackiain 3-O-glucoside (2a) could reach 78.66 μg/mL in a 10-mL system. The study provides efficient catalytic tools for the biosynthesis of pterocarpan glycosides.https://www.maxapress.com/article/doi/10.48130/mpb-0024-0013astragalus membranaceuswhole-cell biocatalysispterocarpan glycosyltransferases |
| spellingShingle | Bai-Han Gao Meng Zhang Kuan Chen Lin-Lin Wang Ming-Ju Yao Hong-Ye Li Ming Xiong Yu-Xi Huang Yang Zhang Quan-Li Liu Juan Guo Min Ye Xue Qiao Characterization of two pterocarpan glycosyltransferases in Astragalus membranaceus and their application in whole-cell biocatalysis Medicinal Plant Biology astragalus membranaceus whole-cell biocatalysis pterocarpan glycosyltransferases |
| title | Characterization of two pterocarpan glycosyltransferases in Astragalus membranaceus and their application in whole-cell biocatalysis |
| title_full | Characterization of two pterocarpan glycosyltransferases in Astragalus membranaceus and their application in whole-cell biocatalysis |
| title_fullStr | Characterization of two pterocarpan glycosyltransferases in Astragalus membranaceus and their application in whole-cell biocatalysis |
| title_full_unstemmed | Characterization of two pterocarpan glycosyltransferases in Astragalus membranaceus and their application in whole-cell biocatalysis |
| title_short | Characterization of two pterocarpan glycosyltransferases in Astragalus membranaceus and their application in whole-cell biocatalysis |
| title_sort | characterization of two pterocarpan glycosyltransferases in astragalus membranaceus and their application in whole cell biocatalysis |
| topic | astragalus membranaceus whole-cell biocatalysis pterocarpan glycosyltransferases |
| url | https://www.maxapress.com/article/doi/10.48130/mpb-0024-0013 |
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