A high-quality genome of Actinidia eriantha provides new insight into ascorbic acid regulation
Actinidia eriantha is one of the species of kiwifruit with a particularly high ascorbic acid (AsA) content. However, the molecular mechanism driving AsA richness in fruit remains unclear. In order to reveal the molecular mechanism of AsA richness in A. eriantha, this study constructed a regulatory n...
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KeAi Communications Co., Ltd.
2023-11-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311923002411 |
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| author | Guang-lian LIAO Chun-hui HUANG Dong-feng JIA Min ZHONG Jun-jie TAO Xue-yan QU Xiao-biao XU |
| author_facet | Guang-lian LIAO Chun-hui HUANG Dong-feng JIA Min ZHONG Jun-jie TAO Xue-yan QU Xiao-biao XU |
| author_sort | Guang-lian LIAO |
| collection | DOAJ |
| description | Actinidia eriantha is one of the species of kiwifruit with a particularly high ascorbic acid (AsA) content. However, the molecular mechanism driving AsA richness in fruit remains unclear. In order to reveal the molecular mechanism of AsA richness in A. eriantha, this study constructed a regulatory network related to AsA metabolism by combining genomics, metabolomics and transcriptomics. We assembled a high-quality genome of A. eriantha ‘Ganlv 1’ with only five remaining gaps. The assembly is comprised of 29 pseudochromosomes with a total size of 615.95 Mb, and contig N50 of 20.35 Mb. Among them, 24 of the pseudochromosomes were obtained directly from telomere-to-telomere. The LTR assembly index score and consensus quality value were 21.34 and 39.90%, respectively. Subsequently, 61 metabolites and 2 092 genes were found to be differentially accumulated/expressed during fruit development by metabolome and transcriptome assays, respectively. AsA metabolism and the cyclic regeneration pathway were found to have high expression levels throughout fruit growth and development, suggesting its crucial role in the regulation of AsA. Furthermore, the AsA contents are highly associated with ascorbate peroxidase genes. The genome obtained in this study provides genomic resources for the genetic and breeding research of A. eriantha, and the constructed regulatory network can provide a public data platform for future research on kiwifruit. |
| format | Article |
| id | doaj-art-6c14a8ba871c4e86bb757a7e2d110f68 |
| institution | Kabale University |
| issn | 2095-3119 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj-art-6c14a8ba871c4e86bb757a7e2d110f682025-08-20T03:56:23ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192023-11-0122113244325510.1016/j.jia.2023.07.018A high-quality genome of Actinidia eriantha provides new insight into ascorbic acid regulationGuang-lian LIAO0Chun-hui HUANG1Dong-feng JIA2Min ZHONG3Jun-jie TAO4Xue-yan QU5Xiao-biao XU6Institute of Kiwifruit, Jiangxi Agricultural University, Nanchang 330045, P.R.ChinaInstitute of Kiwifruit, Jiangxi Agricultural University, Nanchang 330045, P.R.ChinaInstitute of Kiwifruit, Jiangxi Agricultural University, Nanchang 330045, P.R.ChinaInstitute of Kiwifruit, Jiangxi Agricultural University, Nanchang 330045, P.R.ChinaInstitute of Kiwifruit, Jiangxi Agricultural University, Nanchang 330045, P.R.ChinaInstitute of Kiwifruit, Jiangxi Agricultural University, Nanchang 330045, P.R.ChinaCorrespondence XU Xiao-biao, Tel/Fax: +86-791-83813586; Institute of Kiwifruit, Jiangxi Agricultural University, Nanchang 330045, P.R.ChinaActinidia eriantha is one of the species of kiwifruit with a particularly high ascorbic acid (AsA) content. However, the molecular mechanism driving AsA richness in fruit remains unclear. In order to reveal the molecular mechanism of AsA richness in A. eriantha, this study constructed a regulatory network related to AsA metabolism by combining genomics, metabolomics and transcriptomics. We assembled a high-quality genome of A. eriantha ‘Ganlv 1’ with only five remaining gaps. The assembly is comprised of 29 pseudochromosomes with a total size of 615.95 Mb, and contig N50 of 20.35 Mb. Among them, 24 of the pseudochromosomes were obtained directly from telomere-to-telomere. The LTR assembly index score and consensus quality value were 21.34 and 39.90%, respectively. Subsequently, 61 metabolites and 2 092 genes were found to be differentially accumulated/expressed during fruit development by metabolome and transcriptome assays, respectively. AsA metabolism and the cyclic regeneration pathway were found to have high expression levels throughout fruit growth and development, suggesting its crucial role in the regulation of AsA. Furthermore, the AsA contents are highly associated with ascorbate peroxidase genes. The genome obtained in this study provides genomic resources for the genetic and breeding research of A. eriantha, and the constructed regulatory network can provide a public data platform for future research on kiwifruit.http://www.sciencedirect.com/science/article/pii/S2095311923002411Actinidia erianthaascorbic acidnon-targeted metabolomegapless genometranscriptome |
| spellingShingle | Guang-lian LIAO Chun-hui HUANG Dong-feng JIA Min ZHONG Jun-jie TAO Xue-yan QU Xiao-biao XU A high-quality genome of Actinidia eriantha provides new insight into ascorbic acid regulation Journal of Integrative Agriculture Actinidia eriantha ascorbic acid non-targeted metabolome gapless genome transcriptome |
| title | A high-quality genome of Actinidia eriantha provides new insight into ascorbic acid regulation |
| title_full | A high-quality genome of Actinidia eriantha provides new insight into ascorbic acid regulation |
| title_fullStr | A high-quality genome of Actinidia eriantha provides new insight into ascorbic acid regulation |
| title_full_unstemmed | A high-quality genome of Actinidia eriantha provides new insight into ascorbic acid regulation |
| title_short | A high-quality genome of Actinidia eriantha provides new insight into ascorbic acid regulation |
| title_sort | high quality genome of actinidia eriantha provides new insight into ascorbic acid regulation |
| topic | Actinidia eriantha ascorbic acid non-targeted metabolome gapless genome transcriptome |
| url | http://www.sciencedirect.com/science/article/pii/S2095311923002411 |
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