Analysis and characterization of chondroitin/dermatan sulfate composition of lung adenocarcinoma tissues with different types of genetic alterations in ALK, EGFR and KRAS oncogenes
Lung cancer is among the most commonly diagnosed cancer types worldwide. Studying the molecular changes underlying lung cancer is crucial for understanding tumor formation and identifying new therapeutic targets and early markers of the disease to decrease mortality. Investigation of glycosaminoglyc...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666893925001641 |
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| author | Domonkos Pál Fanni Bugyi Dávid Virág Dániel Szabó Gitta Schlosser Ilona Kovalszky Tünde Harkó Judit Moldvay Lilla Turiák |
| author_facet | Domonkos Pál Fanni Bugyi Dávid Virág Dániel Szabó Gitta Schlosser Ilona Kovalszky Tünde Harkó Judit Moldvay Lilla Turiák |
| author_sort | Domonkos Pál |
| collection | DOAJ |
| description | Lung cancer is among the most commonly diagnosed cancer types worldwide. Studying the molecular changes underlying lung cancer is crucial for understanding tumor formation and identifying new therapeutic targets and early markers of the disease to decrease mortality. Investigation of glycosaminoglycan chains offer novel insights, as they play major roles in various signaling events in the tumor microenvironment. In this work we have determined the abundance and sulfation characteristics of chondroitin/dermatan sulfate (CS/DS) glycosaminoglycans in human lung adenocarcinoma tissue sections. The cohort included samples with genetic alterations in key genes such as anaplastic lymphoma kinase (ALK), Kirsten rat sarcoma viral oncogene homolog (KRAS), epidermal growth factor receptor (EGFR) as well as triple wild type samples. CS/DS chains were cleaved using Chondroitinase ABC enzyme and the disaccharides were extracted from the tissue surface and analyzed using liquid chromatography-mass spectrometry. We identified significant changes in the relative abundance of the 4-O-sulfated CS disaccharide between samples with different genetic alterations and in the 6-O-/4-O-sulfation ratio between the ALK and EGFR group. This is the first study comparing CS/DS characteristics in lung adenocarcinoma samples with different genetic alterations and our findings suggest the importance of further testing to understand their role in tumor progression. |
| format | Article |
| id | doaj-art-6c0e40fac8594af1a1901a96902c06be |
| institution | Kabale University |
| issn | 2666-8939 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj-art-6c0e40fac8594af1a1901a96902c06be2025-08-20T03:45:10ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392025-06-011010082610.1016/j.carpta.2025.100826Analysis and characterization of chondroitin/dermatan sulfate composition of lung adenocarcinoma tissues with different types of genetic alterations in ALK, EGFR and KRAS oncogenesDomonkos Pál0Fanni Bugyi1Dávid Virág2Dániel Szabó3Gitta Schlosser4Ilona Kovalszky5Tünde Harkó6Judit Moldvay7Lilla Turiák8MTA-HUN-REN TTK Lendület (Momentum) Glycan Biomarker Research Group, HUN-REN Research Centre for Natural Sciences, H-1117, Magyar tudósok körútja 2., Budapest, Hungary; Semmelweis University, Doctoral School, 1085, Üllői út 26., Budapest, HungaryMTA-HUN-REN TTK Lendület (Momentum) Glycan Biomarker Research Group, HUN-REN Research Centre for Natural Sciences, H-1117, Magyar tudósok körútja 2., Budapest, HungaryMTA-HUN-REN TTK Lendület (Momentum) Glycan Biomarker Research Group, HUN-REN Research Centre for Natural Sciences, H-1117, Magyar tudósok körútja 2., Budapest, HungaryMS Proteomics Research Group, HUN-REN Research Centre for Natural Sciences, H-1117 Magyar tudósok körútja 2., Budapest, HungaryMTA-ELTE Lendület (Momentum) Ion Mobility Mass Spectrometry Research Group, ELTE Eötvös Loránd University, H-1117 Pázmány Péter sétány 1/A, Budapest, HungaryDepartment of Pathology and Experimental Cancer Research, Faculty of Medicine, Semmelweis University, 1085, Üllői út 26., Budapest, HungaryNational Korányi Institute of Pulmonology, Korányi Frigyes út 1, Budapest, H-1121, HungaryNational Korányi Institute of Pulmonology, Korányi Frigyes út 1, Budapest, H-1121, Hungary; Pulmonology Clinic, Albert Szent-Györgyi Medical School, University of Szeged, Alkotmány u. 36., Deszk, H-6771, HungaryMTA-HUN-REN TTK Lendület (Momentum) Glycan Biomarker Research Group, HUN-REN Research Centre for Natural Sciences, H-1117, Magyar tudósok körútja 2., Budapest, Hungary; Corresponding author.Lung cancer is among the most commonly diagnosed cancer types worldwide. Studying the molecular changes underlying lung cancer is crucial for understanding tumor formation and identifying new therapeutic targets and early markers of the disease to decrease mortality. Investigation of glycosaminoglycan chains offer novel insights, as they play major roles in various signaling events in the tumor microenvironment. In this work we have determined the abundance and sulfation characteristics of chondroitin/dermatan sulfate (CS/DS) glycosaminoglycans in human lung adenocarcinoma tissue sections. The cohort included samples with genetic alterations in key genes such as anaplastic lymphoma kinase (ALK), Kirsten rat sarcoma viral oncogene homolog (KRAS), epidermal growth factor receptor (EGFR) as well as triple wild type samples. CS/DS chains were cleaved using Chondroitinase ABC enzyme and the disaccharides were extracted from the tissue surface and analyzed using liquid chromatography-mass spectrometry. We identified significant changes in the relative abundance of the 4-O-sulfated CS disaccharide between samples with different genetic alterations and in the 6-O-/4-O-sulfation ratio between the ALK and EGFR group. This is the first study comparing CS/DS characteristics in lung adenocarcinoma samples with different genetic alterations and our findings suggest the importance of further testing to understand their role in tumor progression.http://www.sciencedirect.com/science/article/pii/S2666893925001641GlycosaminoglycanChondroitin/dermatan sulfateLung adenocarcinomaALKEGFRKRAS |
| spellingShingle | Domonkos Pál Fanni Bugyi Dávid Virág Dániel Szabó Gitta Schlosser Ilona Kovalszky Tünde Harkó Judit Moldvay Lilla Turiák Analysis and characterization of chondroitin/dermatan sulfate composition of lung adenocarcinoma tissues with different types of genetic alterations in ALK, EGFR and KRAS oncogenes Carbohydrate Polymer Technologies and Applications Glycosaminoglycan Chondroitin/dermatan sulfate Lung adenocarcinoma ALK EGFR KRAS |
| title | Analysis and characterization of chondroitin/dermatan sulfate composition of lung adenocarcinoma tissues with different types of genetic alterations in ALK, EGFR and KRAS oncogenes |
| title_full | Analysis and characterization of chondroitin/dermatan sulfate composition of lung adenocarcinoma tissues with different types of genetic alterations in ALK, EGFR and KRAS oncogenes |
| title_fullStr | Analysis and characterization of chondroitin/dermatan sulfate composition of lung adenocarcinoma tissues with different types of genetic alterations in ALK, EGFR and KRAS oncogenes |
| title_full_unstemmed | Analysis and characterization of chondroitin/dermatan sulfate composition of lung adenocarcinoma tissues with different types of genetic alterations in ALK, EGFR and KRAS oncogenes |
| title_short | Analysis and characterization of chondroitin/dermatan sulfate composition of lung adenocarcinoma tissues with different types of genetic alterations in ALK, EGFR and KRAS oncogenes |
| title_sort | analysis and characterization of chondroitin dermatan sulfate composition of lung adenocarcinoma tissues with different types of genetic alterations in alk egfr and kras oncogenes |
| topic | Glycosaminoglycan Chondroitin/dermatan sulfate Lung adenocarcinoma ALK EGFR KRAS |
| url | http://www.sciencedirect.com/science/article/pii/S2666893925001641 |
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