Molecular and genetic features of a bla NDM−1 and bla SHV−12 coharboring hypermucoviscous Klebsiella pneumoniae of serotype K2 and ST65
Abstract Purpose This study aimed to assess the resistance phenotype, virulence phenotype, and genetic characteristics of a bla NDM−1 and bla SHV−12 co-harboring ST65 K2 Klebsiella pneumoniae (KP114), which was isolated from General hospital of Ningxia Medical University. Methods Antibiotic suscepti...
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BMC
2025-03-01
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| Series: | Annals of Clinical Microbiology and Antimicrobials |
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| Online Access: | https://doi.org/10.1186/s12941-025-00789-5 |
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| author | Yuting Kang Qiujie Li Wanting Ma Chao Xu Zhuoran Qiu Wei Jia Pengtao Wang |
| author_facet | Yuting Kang Qiujie Li Wanting Ma Chao Xu Zhuoran Qiu Wei Jia Pengtao Wang |
| author_sort | Yuting Kang |
| collection | DOAJ |
| description | Abstract Purpose This study aimed to assess the resistance phenotype, virulence phenotype, and genetic characteristics of a bla NDM−1 and bla SHV−12 co-harboring ST65 K2 Klebsiella pneumoniae (KP114), which was isolated from General hospital of Ningxia Medical University. Methods Antibiotic susceptibility test was determined by Vitek 2 Compact system. Multilocus Sequence typing (MLST), antimicrobial resistance and virulence genes were examined by PCR and Sanger sequencing. The virulence of KP114 was evaluated through string test, macrophage phagocytosis assay, serum resistance assay, and mouse infection model. Whole-genome sequencing was performed for further analysis of genetic information. Results The presence of the bla NDM−1 and bla SHV−12 genes in KP114 confered resistance to multi-antibiotics. The hypervirulence of KP114 was demonstrated through various in vitro experiments and in vivo mouse infection model. KP114 was found to harbor two distinct plasmids: a drug-resistant plasmid (pKP114-NDM), classified as the IncX3 type, which contained various transfer elements including type IV coupling protein (T4CP) and type IV secretion system (T4SS), and a virulence plasmid (pKP114-vir) that exhibited a high sequence similarity with pLVPK. The results of the conjugation experiment showed that resistance and virulence traits were successfully transferred from KP114 to Escherichia coli EC600 and J53. Conclusions We reported a Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) strain of ST65 K2 serotype carrying the bla NDM−1 and bla SHV−12, which exhibited hypervirulence and drug resistance with potential for transmission. This finding allows improved clinical surveillance and control of this clone, thereby holding considerable value for clinical treatment. |
| format | Article |
| id | doaj-art-6c0cbc2ca93d4867b974c407fbb5a90e |
| institution | DOAJ |
| issn | 1476-0711 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Annals of Clinical Microbiology and Antimicrobials |
| spelling | doaj-art-6c0cbc2ca93d4867b974c407fbb5a90e2025-08-20T02:51:23ZengBMCAnnals of Clinical Microbiology and Antimicrobials1476-07112025-03-0124111410.1186/s12941-025-00789-5Molecular and genetic features of a bla NDM−1 and bla SHV−12 coharboring hypermucoviscous Klebsiella pneumoniae of serotype K2 and ST65Yuting Kang0Qiujie Li1Wanting Ma2Chao Xu3Zhuoran Qiu4Wei Jia5Pengtao Wang6Ningxia Key Laboratory of Clinical and Pathogenic Microbiology, Institute of Medical Sciences, General Hospital of Ningxia Medical UniversityFirst Clinical Medical College, Ningxia Medical UniversityFirst Clinical Medical College, Ningxia Medical UniversityFirst Clinical Medical College, Ningxia Medical UniversityFirst Clinical Medical College, Ningxia Medical UniversityNingxia Key Laboratory of Clinical and Pathogenic Microbiology, Institute of Medical Sciences, General Hospital of Ningxia Medical UniversityNingxia Key Laboratory of Clinical and Pathogenic Microbiology, Institute of Medical Sciences, General Hospital of Ningxia Medical UniversityAbstract Purpose This study aimed to assess the resistance phenotype, virulence phenotype, and genetic characteristics of a bla NDM−1 and bla SHV−12 co-harboring ST65 K2 Klebsiella pneumoniae (KP114), which was isolated from General hospital of Ningxia Medical University. Methods Antibiotic susceptibility test was determined by Vitek 2 Compact system. Multilocus Sequence typing (MLST), antimicrobial resistance and virulence genes were examined by PCR and Sanger sequencing. The virulence of KP114 was evaluated through string test, macrophage phagocytosis assay, serum resistance assay, and mouse infection model. Whole-genome sequencing was performed for further analysis of genetic information. Results The presence of the bla NDM−1 and bla SHV−12 genes in KP114 confered resistance to multi-antibiotics. The hypervirulence of KP114 was demonstrated through various in vitro experiments and in vivo mouse infection model. KP114 was found to harbor two distinct plasmids: a drug-resistant plasmid (pKP114-NDM), classified as the IncX3 type, which contained various transfer elements including type IV coupling protein (T4CP) and type IV secretion system (T4SS), and a virulence plasmid (pKP114-vir) that exhibited a high sequence similarity with pLVPK. The results of the conjugation experiment showed that resistance and virulence traits were successfully transferred from KP114 to Escherichia coli EC600 and J53. Conclusions We reported a Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) strain of ST65 K2 serotype carrying the bla NDM−1 and bla SHV−12, which exhibited hypervirulence and drug resistance with potential for transmission. This finding allows improved clinical surveillance and control of this clone, thereby holding considerable value for clinical treatment.https://doi.org/10.1186/s12941-025-00789-5Klebsiella pneumoniaeST65Serotype K2bla NDM−1Drug resistanceVirulence plasmid |
| spellingShingle | Yuting Kang Qiujie Li Wanting Ma Chao Xu Zhuoran Qiu Wei Jia Pengtao Wang Molecular and genetic features of a bla NDM−1 and bla SHV−12 coharboring hypermucoviscous Klebsiella pneumoniae of serotype K2 and ST65 Annals of Clinical Microbiology and Antimicrobials Klebsiella pneumoniae ST65 Serotype K2 bla NDM−1 Drug resistance Virulence plasmid |
| title | Molecular and genetic features of a bla NDM−1 and bla SHV−12 coharboring hypermucoviscous Klebsiella pneumoniae of serotype K2 and ST65 |
| title_full | Molecular and genetic features of a bla NDM−1 and bla SHV−12 coharboring hypermucoviscous Klebsiella pneumoniae of serotype K2 and ST65 |
| title_fullStr | Molecular and genetic features of a bla NDM−1 and bla SHV−12 coharboring hypermucoviscous Klebsiella pneumoniae of serotype K2 and ST65 |
| title_full_unstemmed | Molecular and genetic features of a bla NDM−1 and bla SHV−12 coharboring hypermucoviscous Klebsiella pneumoniae of serotype K2 and ST65 |
| title_short | Molecular and genetic features of a bla NDM−1 and bla SHV−12 coharboring hypermucoviscous Klebsiella pneumoniae of serotype K2 and ST65 |
| title_sort | molecular and genetic features of a bla ndm 1 and bla shv 12 coharboring hypermucoviscous klebsiella pneumoniae of serotype k2 and st65 |
| topic | Klebsiella pneumoniae ST65 Serotype K2 bla NDM−1 Drug resistance Virulence plasmid |
| url | https://doi.org/10.1186/s12941-025-00789-5 |
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