Prognostic value of microRNA assessment in cervical epithelial cells of patients with mild dysplasia
Background. Currently, there are no reliable markers for the prognosis of the low-grade squamous intraepithelial lesion (LSIL) of the cervical epithelium. Scientific literature provides with inconsistent recommendations regarding the management of the young patients with a cytological diagnosis of L...
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ABV-press
2021-03-01
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| Series: | Опухоли женской репродуктивной системы |
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| Online Access: | https://ojrs.abvpress.ru/ojrs/article/view/779 |
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| author | M. S. Knyazeva Т. S. Prisyazhnaya L. M. Zabegina О. A. Smirnova A. A. Mikhetko I. V. Berlev A. V. Malek |
| author_facet | M. S. Knyazeva Т. S. Prisyazhnaya L. M. Zabegina О. A. Smirnova A. A. Mikhetko I. V. Berlev A. V. Malek |
| author_sort | M. S. Knyazeva |
| collection | DOAJ |
| description | Background. Currently, there are no reliable markers for the prognosis of the low-grade squamous intraepithelial lesion (LSIL) of the cervical epithelium. Scientific literature provides with inconsistent recommendations regarding the management of the young patients with a cytological diagnosis of LSIL. The progression of cervical dysplasia and the development of cervical cancer are associated with characteristic alterations of the microRNA expression profile.Objective: to assess the prognostic value of microRNA in LSIL. Materials and methods. Samples (cytological smears) obtained from patients diagnosed with LSIL (n = 36), but with a different course of the disease over the next 6–12 months. Analysis of miRNA expression was carried out by the method of “two-tailed” reverse transcription and subsequent PCR.Results. The expression level of miR-126, miR-21, miR-1246, miR-182 was statistically significantly different in the compared groups, but the predictive value of the analysis of individual molecules was low (AUC <0.65). Calculation of the concentration ratios of the “reciprocal” pairs of microRNAs made it possible to obtain a more effective prognostic marker. ROC analysis of such ratios (miR-126/miR-182, miR-21/miR-182, miR-1246/miR-182) yielded AUC values: 0.82–0.89, sensitivity: 0.71–0.92; specificity: 0.86.Conclusions. Analysis of a panel of microRNA marker molecules in the material of the cervical epithelium and calculation of the concentration ratios of “reciprocal” pairs is a promising method for prognosis of LSIL course. |
| format | Article |
| id | doaj-art-6c0be9abe19a41d2886ab00a59f0c532 |
| institution | Kabale University |
| issn | 1994-4098 1999-8627 |
| language | Russian |
| publishDate | 2021-03-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Опухоли женской репродуктивной системы |
| spelling | doaj-art-6c0be9abe19a41d2886ab00a59f0c5322025-08-20T03:37:53ZrusABV-pressОпухоли женской репродуктивной системы1994-40981999-86272021-03-01164667510.17650/1994-4098-2020-16-4-66-75667Prognostic value of microRNA assessment in cervical epithelial cells of patients with mild dysplasiaM. S. Knyazeva0Т. S. Prisyazhnaya1L. M. Zabegina2О. A. Smirnova3A. A. Mikhetko4I. V. Berlev5A. V. Malek6N.N. Petrov National Research Institute of Oncology, Ministry of Health of Russia; Oncosystem LLCI.I. Mechnikov North-Western State Medical University, Ministry of Health of RussiaN.N. Petrov National Research Institute of Oncology, Ministry of Health of Russia; Oncosystem LLCN.N. Petrov National Research Institute of Oncology, Ministry of Health of RussiaN.N. Petrov National Research Institute of Oncology, Ministry of Health of RussiaN.N. Petrov National Research Institute of Oncology, Ministry of Health of Russia; I.I. Mechnikov North-Western State Medical University, Ministry of Health of RussiaN.N. Petrov National Research Institute of Oncology, Ministry of Health of Russia; Oncosystem LLC;Background. Currently, there are no reliable markers for the prognosis of the low-grade squamous intraepithelial lesion (LSIL) of the cervical epithelium. Scientific literature provides with inconsistent recommendations regarding the management of the young patients with a cytological diagnosis of LSIL. The progression of cervical dysplasia and the development of cervical cancer are associated with characteristic alterations of the microRNA expression profile.Objective: to assess the prognostic value of microRNA in LSIL. Materials and methods. Samples (cytological smears) obtained from patients diagnosed with LSIL (n = 36), but with a different course of the disease over the next 6–12 months. Analysis of miRNA expression was carried out by the method of “two-tailed” reverse transcription and subsequent PCR.Results. The expression level of miR-126, miR-21, miR-1246, miR-182 was statistically significantly different in the compared groups, but the predictive value of the analysis of individual molecules was low (AUC <0.65). Calculation of the concentration ratios of the “reciprocal” pairs of microRNAs made it possible to obtain a more effective prognostic marker. ROC analysis of such ratios (miR-126/miR-182, miR-21/miR-182, miR-1246/miR-182) yielded AUC values: 0.82–0.89, sensitivity: 0.71–0.92; specificity: 0.86.Conclusions. Analysis of a panel of microRNA marker molecules in the material of the cervical epithelium and calculation of the concentration ratios of “reciprocal” pairs is a promising method for prognosis of LSIL course.https://ojrs.abvpress.ru/ojrs/article/view/779cervical cancerlow-grade squamous intraepithelial lesionprognosismicrornareverse transcription polymerase chain reaction |
| spellingShingle | M. S. Knyazeva Т. S. Prisyazhnaya L. M. Zabegina О. A. Smirnova A. A. Mikhetko I. V. Berlev A. V. Malek Prognostic value of microRNA assessment in cervical epithelial cells of patients with mild dysplasia Опухоли женской репродуктивной системы cervical cancer low-grade squamous intraepithelial lesion prognosis microrna reverse transcription polymerase chain reaction |
| title | Prognostic value of microRNA assessment in cervical epithelial cells of patients with mild dysplasia |
| title_full | Prognostic value of microRNA assessment in cervical epithelial cells of patients with mild dysplasia |
| title_fullStr | Prognostic value of microRNA assessment in cervical epithelial cells of patients with mild dysplasia |
| title_full_unstemmed | Prognostic value of microRNA assessment in cervical epithelial cells of patients with mild dysplasia |
| title_short | Prognostic value of microRNA assessment in cervical epithelial cells of patients with mild dysplasia |
| title_sort | prognostic value of microrna assessment in cervical epithelial cells of patients with mild dysplasia |
| topic | cervical cancer low-grade squamous intraepithelial lesion prognosis microrna reverse transcription polymerase chain reaction |
| url | https://ojrs.abvpress.ru/ojrs/article/view/779 |
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