Molecular Diagnosis In Infectious Keratitis

Microbial keratitis is a very important public health problem in our country. The magnitude of the burden of this problem is not often fully understood and hence it is often termed as a “silent epidemic”. The affected population are often the vulnerable members of the society like people working in...

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Main Authors: Venkatesh Prajna N, Lokeshwari A, Lalitha Prajna
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2014-04-01
Series:Delhi Journal of Ophthalmology
Subjects:
Online Access:https://journals.lww.com/10.7869/djo.55
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author Venkatesh Prajna N
Lokeshwari A
Lalitha Prajna
author_facet Venkatesh Prajna N
Lokeshwari A
Lalitha Prajna
author_sort Venkatesh Prajna N
collection DOAJ
description Microbial keratitis is a very important public health problem in our country. The magnitude of the burden of this problem is not often fully understood and hence it is often termed as a “silent epidemic”. The affected population are often the vulnerable members of the society like people working in fields, warehouses and manual unskilled labour intensive activities. Very often, these affected individuals delay their presentation to an ophthalmologist and often indulge in self medications. Even though classic clinical features have been described for various aetiological organisms causing keratitis, it is extremely difficult to accurately predict the same by clinical examination alone. Microbiological support systems are extremely important and mandatory for diagnosing and ultimately treating this problem. While routine microbiological investigations like smears and cultures remain the backbone of the armamentarium, newer molecular tests such as polymerase chain reaction (PCR) tests are useful in certain indications. These tests are useful to detect slow growing organisms, fastidious organisms and may offer a comparative quick diagnosis. This review offers the current applications of the use of these molecular tests with reference to suppurative microbial keratitis.
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issn 0972-0200
2454-2784
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publishDate 2014-04-01
publisher Wolters Kluwer Medknow Publications
record_format Article
series Delhi Journal of Ophthalmology
spelling doaj-art-6c09df20e68644d288f576c3b301fccf2025-08-20T03:11:07ZengWolters Kluwer Medknow PublicationsDelhi Journal of Ophthalmology0972-02002454-27842014-04-0124425826210.7869/djo.55Molecular Diagnosis In Infectious KeratitisVenkatesh Prajna NLokeshwari ALalitha PrajnaMicrobial keratitis is a very important public health problem in our country. The magnitude of the burden of this problem is not often fully understood and hence it is often termed as a “silent epidemic”. The affected population are often the vulnerable members of the society like people working in fields, warehouses and manual unskilled labour intensive activities. Very often, these affected individuals delay their presentation to an ophthalmologist and often indulge in self medications. Even though classic clinical features have been described for various aetiological organisms causing keratitis, it is extremely difficult to accurately predict the same by clinical examination alone. Microbiological support systems are extremely important and mandatory for diagnosing and ultimately treating this problem. While routine microbiological investigations like smears and cultures remain the backbone of the armamentarium, newer molecular tests such as polymerase chain reaction (PCR) tests are useful in certain indications. These tests are useful to detect slow growing organisms, fastidious organisms and may offer a comparative quick diagnosis. This review offers the current applications of the use of these molecular tests with reference to suppurative microbial keratitis.https://journals.lww.com/10.7869/djo.55pcrpolymerase chain reactionmolecular diagnosis
spellingShingle Venkatesh Prajna N
Lokeshwari A
Lalitha Prajna
Molecular Diagnosis In Infectious Keratitis
Delhi Journal of Ophthalmology
pcr
polymerase chain reaction
molecular diagnosis
title Molecular Diagnosis In Infectious Keratitis
title_full Molecular Diagnosis In Infectious Keratitis
title_fullStr Molecular Diagnosis In Infectious Keratitis
title_full_unstemmed Molecular Diagnosis In Infectious Keratitis
title_short Molecular Diagnosis In Infectious Keratitis
title_sort molecular diagnosis in infectious keratitis
topic pcr
polymerase chain reaction
molecular diagnosis
url https://journals.lww.com/10.7869/djo.55
work_keys_str_mv AT venkateshprajnan moleculardiagnosisininfectiouskeratitis
AT lokeshwaria moleculardiagnosisininfectiouskeratitis
AT lalithaprajna moleculardiagnosisininfectiouskeratitis