Untargeted Chemical Profile, Antioxidant, and Enzyme Inhibition Activity of <i>Physalis angulata</i> L. from the Peruvian Amazon: A Contribution to the Validation of Its Pharmacological Potential
<i>Physalis angulata</i> is a plant of great value in traditional medicine known for its content of bioactive compounds, such as physalins and withanolides, which possess diverse biological activities. In this study, the chemical profile, antioxidant activity, and enzyme inhibition capac...
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2025-02-01
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| Series: | Antioxidants |
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| author | Gabriel Vargas-Arana Alfredo Torres-Benítez José Erick Ortega-Valencia Claudia Merino-Zegarra Pilar Carranza-Rosales Mario J. Simirgiotis |
| author_facet | Gabriel Vargas-Arana Alfredo Torres-Benítez José Erick Ortega-Valencia Claudia Merino-Zegarra Pilar Carranza-Rosales Mario J. Simirgiotis |
| author_sort | Gabriel Vargas-Arana |
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| description | <i>Physalis angulata</i> is a plant of great value in traditional medicine known for its content of bioactive compounds, such as physalins and withanolides, which possess diverse biological activities. In this study, the chemical profile, antioxidant activity, and enzyme inhibition capacity of aqueous and ethanolic extracts obtained from the root, stem, leaves, calyx, and fruits of <i>P. angulata</i> collected in Peru were evaluated. A total of forty-two compounds were detected in the extracts using UHPLC-ESI-QTOF-MS analysis. In vitro analyses revealed that leaf extracts contained the highest concentration of phenolic compounds, while leaf and fruit extracts showed the best results in FRAP, DPPH, and ABTS antioxidant tests; on the other hand, inhibition of AChE, BChE, α-glucosidase, and α-amylase enzymes was variable, but calyx and fruit extracts showed higher effectiveness. In silico analyses indicated that the compounds physagulin A, physagulin F, physagulide P, physalin B, and withaminimin showed stable interactions and favorable binding affinities with the catalytic sites of the enzymes studied. These results confirm the pharmacological potential of extracts and compounds derived from different organs of <i>P. angulata</i>, suggesting their promising use in treating diseases related to the central nervous system and metabolic syndrome. |
| format | Article |
| id | doaj-art-6c09ba7ade854b3e8e4a5ce15166fe1a |
| institution | Kabale University |
| issn | 2076-3921 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
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| series | Antioxidants |
| spelling | doaj-art-6c09ba7ade854b3e8e4a5ce15166fe1a2025-08-20T03:40:43ZengMDPI AGAntioxidants2076-39212025-02-0114324610.3390/antiox14030246Untargeted Chemical Profile, Antioxidant, and Enzyme Inhibition Activity of <i>Physalis angulata</i> L. from the Peruvian Amazon: A Contribution to the Validation of Its Pharmacological PotentialGabriel Vargas-Arana0Alfredo Torres-Benítez1José Erick Ortega-Valencia2Claudia Merino-Zegarra3Pilar Carranza-Rosales4Mario J. Simirgiotis5Laboratorio de Química de Productos Naturales, Instituto de Investigaciones de la Amazonía Peruana, Avenue Abelardo Quiñones Km 2.5, Iquitos 16001, PeruCarrera de Química y Farmacia, Facultad de Ciencias, Universidad San Sebastián, General Lagos 1163, Valdivia 5090000, ChileTecnológico Nacional de México, Instituto Tecnológico Superior de Xalapa, Sección 5ª Reserva Territorial S/N, Col. Santa Bárbara 91096, Veracruz, MexicoLaboratorio de Química de Productos Naturales, Instituto de Investigaciones de la Amazonía Peruana, Avenue Abelardo Quiñones Km 2.5, Iquitos 16001, PeruCentro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Monterrey 64720, Nuevo León, MexicoInstituto de Farmacia, Facultad de Ciencias, Universidad Austral de Chile, Campus Isla Teja, Valdivia 5090000, Chile<i>Physalis angulata</i> is a plant of great value in traditional medicine known for its content of bioactive compounds, such as physalins and withanolides, which possess diverse biological activities. In this study, the chemical profile, antioxidant activity, and enzyme inhibition capacity of aqueous and ethanolic extracts obtained from the root, stem, leaves, calyx, and fruits of <i>P. angulata</i> collected in Peru were evaluated. A total of forty-two compounds were detected in the extracts using UHPLC-ESI-QTOF-MS analysis. In vitro analyses revealed that leaf extracts contained the highest concentration of phenolic compounds, while leaf and fruit extracts showed the best results in FRAP, DPPH, and ABTS antioxidant tests; on the other hand, inhibition of AChE, BChE, α-glucosidase, and α-amylase enzymes was variable, but calyx and fruit extracts showed higher effectiveness. In silico analyses indicated that the compounds physagulin A, physagulin F, physagulide P, physalin B, and withaminimin showed stable interactions and favorable binding affinities with the catalytic sites of the enzymes studied. These results confirm the pharmacological potential of extracts and compounds derived from different organs of <i>P. angulata</i>, suggesting their promising use in treating diseases related to the central nervous system and metabolic syndrome.https://www.mdpi.com/2076-3921/14/3/246<i>Physalis angulata</i>extractsbioactive compoundsbiological activitytherapeutic properties |
| spellingShingle | Gabriel Vargas-Arana Alfredo Torres-Benítez José Erick Ortega-Valencia Claudia Merino-Zegarra Pilar Carranza-Rosales Mario J. Simirgiotis Untargeted Chemical Profile, Antioxidant, and Enzyme Inhibition Activity of <i>Physalis angulata</i> L. from the Peruvian Amazon: A Contribution to the Validation of Its Pharmacological Potential Antioxidants <i>Physalis angulata</i> extracts bioactive compounds biological activity therapeutic properties |
| title | Untargeted Chemical Profile, Antioxidant, and Enzyme Inhibition Activity of <i>Physalis angulata</i> L. from the Peruvian Amazon: A Contribution to the Validation of Its Pharmacological Potential |
| title_full | Untargeted Chemical Profile, Antioxidant, and Enzyme Inhibition Activity of <i>Physalis angulata</i> L. from the Peruvian Amazon: A Contribution to the Validation of Its Pharmacological Potential |
| title_fullStr | Untargeted Chemical Profile, Antioxidant, and Enzyme Inhibition Activity of <i>Physalis angulata</i> L. from the Peruvian Amazon: A Contribution to the Validation of Its Pharmacological Potential |
| title_full_unstemmed | Untargeted Chemical Profile, Antioxidant, and Enzyme Inhibition Activity of <i>Physalis angulata</i> L. from the Peruvian Amazon: A Contribution to the Validation of Its Pharmacological Potential |
| title_short | Untargeted Chemical Profile, Antioxidant, and Enzyme Inhibition Activity of <i>Physalis angulata</i> L. from the Peruvian Amazon: A Contribution to the Validation of Its Pharmacological Potential |
| title_sort | untargeted chemical profile antioxidant and enzyme inhibition activity of i physalis angulata i l from the peruvian amazon a contribution to the validation of its pharmacological potential |
| topic | <i>Physalis angulata</i> extracts bioactive compounds biological activity therapeutic properties |
| url | https://www.mdpi.com/2076-3921/14/3/246 |
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