Effective <i>Agrobacterium</i>-Mediated Transformation System for Eureka Lemon Using Whole Cotyledonary Node
<i>Agrobacterium</i>-mediated transformation systems using epicotyl explants have been widely used for genetic transformations of citrus. However, their application in lemons is severely constrained by browning of epicotyl tissues, which leads to an extremely low efficiency of transforma...
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MDPI AG
2025-05-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/14/11/1629 |
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| author | Jinfa Zhao Yuan Chen Jiajun Wang Chunqing Wang Yan Zhou |
| author_facet | Jinfa Zhao Yuan Chen Jiajun Wang Chunqing Wang Yan Zhou |
| author_sort | Jinfa Zhao |
| collection | DOAJ |
| description | <i>Agrobacterium</i>-mediated transformation systems using epicotyl explants have been widely used for genetic transformations of citrus. However, their application in lemons is severely constrained by browning of epicotyl tissues, which leads to an extremely low efficiency of transformation. In this study, we developed an optimized <i>Agrobacterium</i>-mediated transformation system using whole cotyledonary node explants of ‘Eureka’ lemon (<i>Citrus limon</i>), which significantly reduced tissue browning and enhanced transformation efficiency up to 14.48%. In addition, preparation of the whole cotyledonary node was simple and rapid, which reduced time and labor. This system facilitated efficient generation of transgenic lemon plantlets and provided a novel explant source for citrus transformation. |
| format | Article |
| id | doaj-art-6c0923cd33bf41a0b2d1b251670cf571 |
| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-05-01 |
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| series | Plants |
| spelling | doaj-art-6c0923cd33bf41a0b2d1b251670cf5712025-08-20T03:46:45ZengMDPI AGPlants2223-77472025-05-011411162910.3390/plants14111629Effective <i>Agrobacterium</i>-Mediated Transformation System for Eureka Lemon Using Whole Cotyledonary NodeJinfa Zhao0Yuan Chen1Jiajun Wang2Chunqing Wang3Yan Zhou4Integrative Science Center of Germplasm Creation in Western China (Chongqing), Science City/Citrus Research Institute, Southwest University, Chongqing 400712, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing), Science City/Citrus Research Institute, Southwest University, Chongqing 400712, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing), Science City/Citrus Research Institute, Southwest University, Chongqing 400712, ChinaInstitute of Plant Protection, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, ChinaIntegrative Science Center of Germplasm Creation in Western China (Chongqing), Science City/Citrus Research Institute, Southwest University, Chongqing 400712, China<i>Agrobacterium</i>-mediated transformation systems using epicotyl explants have been widely used for genetic transformations of citrus. However, their application in lemons is severely constrained by browning of epicotyl tissues, which leads to an extremely low efficiency of transformation. In this study, we developed an optimized <i>Agrobacterium</i>-mediated transformation system using whole cotyledonary node explants of ‘Eureka’ lemon (<i>Citrus limon</i>), which significantly reduced tissue browning and enhanced transformation efficiency up to 14.48%. In addition, preparation of the whole cotyledonary node was simple and rapid, which reduced time and labor. This system facilitated efficient generation of transgenic lemon plantlets and provided a novel explant source for citrus transformation.https://www.mdpi.com/2223-7747/14/11/1629lemon transformationwhole cotyledonary nodeexplanttransformation efficiency |
| spellingShingle | Jinfa Zhao Yuan Chen Jiajun Wang Chunqing Wang Yan Zhou Effective <i>Agrobacterium</i>-Mediated Transformation System for Eureka Lemon Using Whole Cotyledonary Node Plants lemon transformation whole cotyledonary node explant transformation efficiency |
| title | Effective <i>Agrobacterium</i>-Mediated Transformation System for Eureka Lemon Using Whole Cotyledonary Node |
| title_full | Effective <i>Agrobacterium</i>-Mediated Transformation System for Eureka Lemon Using Whole Cotyledonary Node |
| title_fullStr | Effective <i>Agrobacterium</i>-Mediated Transformation System for Eureka Lemon Using Whole Cotyledonary Node |
| title_full_unstemmed | Effective <i>Agrobacterium</i>-Mediated Transformation System for Eureka Lemon Using Whole Cotyledonary Node |
| title_short | Effective <i>Agrobacterium</i>-Mediated Transformation System for Eureka Lemon Using Whole Cotyledonary Node |
| title_sort | effective i agrobacterium i mediated transformation system for eureka lemon using whole cotyledonary node |
| topic | lemon transformation whole cotyledonary node explant transformation efficiency |
| url | https://www.mdpi.com/2223-7747/14/11/1629 |
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