Pharmacogenetic exploration of buprenorphine and related metabolites in umbilical cord blood

The goal of this study was to explore associations between single-nucleotide polymorphisms (SNPs) and umbilical cord blood concentrations of buprenorphine and its metabolites following maternal administration. This is a sub-study of a prospective observational cohort investigation which included pre...

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Main Authors: Amelia Monfared, Derek E. Murrell, Darshan S. Shah, Melissa Hoang, Stacy D. Brown, Sam Harirforoosh
Format: Article
Language:English
Published: Elsevier 2025-12-01
Series:Toxicology Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S2214750025002112
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author Amelia Monfared
Derek E. Murrell
Darshan S. Shah
Melissa Hoang
Stacy D. Brown
Sam Harirforoosh
author_facet Amelia Monfared
Derek E. Murrell
Darshan S. Shah
Melissa Hoang
Stacy D. Brown
Sam Harirforoosh
author_sort Amelia Monfared
collection DOAJ
description The goal of this study was to explore associations between single-nucleotide polymorphisms (SNPs) and umbilical cord blood concentrations of buprenorphine and its metabolites following maternal administration. This is a sub-study of a prospective observational cohort investigation which included pregnant women receiving buprenorphine for opioid use disorder during pregnancy. Following delivery, umbilical cord blood samples were collected and genotyped using a pharmacogenetic panel. The drug and metabolite concentrations were analyzed through liquid chromatography-mass spectrometry, and genetic association analysis was completed using PLINK software. The included neonates (n = 14) had a mean birth weight of 3.00 ± 0.39 kg and were born to mothers receiving a mean buprenorphine dose of 10.29 ± 6.22 mg. Ten concentration groupings (drug, single metabolite, as well as drug/metabolite(s) combinations) produced 18 unique SNP associations. Two significant associations included variations in CYP3A4 and UGT1A1, which play a role in the metabolism of buprenorphine. These preliminary findings suggest potential pharmacogenetic factors influencing fetal drug exposure, warranting larger studies to validate associations and explore clinical implications for neonatal outcomes.
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spelling doaj-art-6c04aa4633024e3eb364bb5e68ef502e2025-08-20T03:58:41ZengElsevierToxicology Reports2214-75002025-12-011510209310.1016/j.toxrep.2025.102093Pharmacogenetic exploration of buprenorphine and related metabolites in umbilical cord bloodAmelia Monfared0Derek E. Murrell1Darshan S. Shah2Melissa Hoang3Stacy D. Brown4Sam Harirforoosh5School of Pharmacy, Chapman University, Irvine, CA, USAGatton College of Pharmacy, East Tennessee State University, Johnson City, TN, USAQuillen College of Medicine, East Tennessee State University, Johnson City, TN, USASchool of Pharmacy, Chapman University, Irvine, CA, USAGatton College of Pharmacy, East Tennessee State University, Johnson City, TN, USASchool of Pharmacy, Chapman University, Irvine, CA, USA; Correspondence to: Department of Biomedical and Pharmaceutical Sciences, School of Pharmacy, Chapman University, Irvine, CA 92618, USA.The goal of this study was to explore associations between single-nucleotide polymorphisms (SNPs) and umbilical cord blood concentrations of buprenorphine and its metabolites following maternal administration. This is a sub-study of a prospective observational cohort investigation which included pregnant women receiving buprenorphine for opioid use disorder during pregnancy. Following delivery, umbilical cord blood samples were collected and genotyped using a pharmacogenetic panel. The drug and metabolite concentrations were analyzed through liquid chromatography-mass spectrometry, and genetic association analysis was completed using PLINK software. The included neonates (n = 14) had a mean birth weight of 3.00 ± 0.39 kg and were born to mothers receiving a mean buprenorphine dose of 10.29 ± 6.22 mg. Ten concentration groupings (drug, single metabolite, as well as drug/metabolite(s) combinations) produced 18 unique SNP associations. Two significant associations included variations in CYP3A4 and UGT1A1, which play a role in the metabolism of buprenorphine. These preliminary findings suggest potential pharmacogenetic factors influencing fetal drug exposure, warranting larger studies to validate associations and explore clinical implications for neonatal outcomes.http://www.sciencedirect.com/science/article/pii/S2214750025002112PharmacogeneticsPolymorphismBuprenorphineMetabolitesUmbilical cordNeonatal abstinence syndrome
spellingShingle Amelia Monfared
Derek E. Murrell
Darshan S. Shah
Melissa Hoang
Stacy D. Brown
Sam Harirforoosh
Pharmacogenetic exploration of buprenorphine and related metabolites in umbilical cord blood
Toxicology Reports
Pharmacogenetics
Polymorphism
Buprenorphine
Metabolites
Umbilical cord
Neonatal abstinence syndrome
title Pharmacogenetic exploration of buprenorphine and related metabolites in umbilical cord blood
title_full Pharmacogenetic exploration of buprenorphine and related metabolites in umbilical cord blood
title_fullStr Pharmacogenetic exploration of buprenorphine and related metabolites in umbilical cord blood
title_full_unstemmed Pharmacogenetic exploration of buprenorphine and related metabolites in umbilical cord blood
title_short Pharmacogenetic exploration of buprenorphine and related metabolites in umbilical cord blood
title_sort pharmacogenetic exploration of buprenorphine and related metabolites in umbilical cord blood
topic Pharmacogenetics
Polymorphism
Buprenorphine
Metabolites
Umbilical cord
Neonatal abstinence syndrome
url http://www.sciencedirect.com/science/article/pii/S2214750025002112
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AT melissahoang pharmacogeneticexplorationofbuprenorphineandrelatedmetabolitesinumbilicalcordblood
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