Exploring transcriptomic databases to identify and experimentally validate tissue-specific consensus reference gene for gene expression normalization in BALB/c mice acutely exposed to 2,3,7,8-Tetrachlorodibenzo-p-dioxin

Exploring transcriptomic databases to identify and experimentally validate tissue-specific consensus reference gene for gene expression normalization in BALB/c mice acutely exposed to 2,3,7,8-Tetrachlorodibenzo-p-dioxin

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a toxic compound affecting organs like the liver, kidney, lung, and reproductive systems in mammals. This study outlines a strategy for choosing appropriate HKGs for tissue-specific gene expression analysis in TCDD toxicity, including four steps: i) iden...

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Bibliographic Details
Main Authors: Nour Hammoudeh, Reem Hasan, Mohammad Deeb, Zuher Radwan, Omar Ayoubi, Roaa Alendary, Mouayad Youssef, Abdulfattah Kazan, Rasil Alsahli, Walaa Faiad, Nour Aldeli, Abdulsamie Hanano
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:Current Research in Toxicology
Subjects:
Dioxin
Gene expression
Mouse BALB/C
Housekeeping genes
RT-qPCR
Online Access:http://www.sciencedirect.com/science/article/pii/S2666027X25000209
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