Screening, identification and enzyme production conditions optimization of a cellulase-producing yeast
In order to screen high cellulose-producing yeast, cellulose-producing yeasts were screened from the fermented grains of sauce-flavor (Jiangxiangxing) Baijiu by Rose Bengal Agar and Congo Red Medium, and the screened strains were identified by morphological observation, physiological and biochemical...
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Editorial Department of China Brewing
2024-09-01
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| Series: | Zhongguo niangzao |
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| Online Access: | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-9-72.pdf |
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| author | RAN Guangyao, TANG Jiadai, ZHAO Yimei, MENG Zhuoni, LONG Yafei, GUO Min, GUO Ju |
| author_facet | RAN Guangyao, TANG Jiadai, ZHAO Yimei, MENG Zhuoni, LONG Yafei, GUO Min, GUO Ju |
| author_sort | RAN Guangyao, TANG Jiadai, ZHAO Yimei, MENG Zhuoni, LONG Yafei, GUO Min, GUO Ju |
| collection | DOAJ |
| description | In order to screen high cellulose-producing yeast, cellulose-producing yeasts were screened from the fermented grains of sauce-flavor (Jiangxiangxing) Baijiu by Rose Bengal Agar and Congo Red Medium, and the screened strains were identified by morphological observation, physiological and biochemical tests, and molecular biology technique. The tolerance was investigated and the enzyme production conditions were optimized. The results showed that a cellulase-producing strain JXJP-Y19 was screened, and the strain JXJP-Y19 was identified as Yarrowia lipolytica. The temperature, ethanol, and pH tolerance of the strain JXJP-Y19 were 39 ℃, 8%(V/V), and 3, respectively, showing good tolerance to environmental stress. The optimal enzyme production conditions were as follows: bran as carbon source, cottonseed cake as nitrogen source, inoculum 1%, initial pH 6 and fermentation temperature 30 ℃. Under these optimal conditions, the activities of filter paper enzyme, exoglucanase and β-glucosidase produced by the strain JXJP-Y19 were (0.913±0.025) U/ml, (1.272±0.025) U/ml and (0.937±0.030) U/ml, respectively, which were 2.15 times, 1.81 times, and 1.94 times higher than before optimization. The strain JXJP-Y19 showed good cellulase activity and could be used as a potential strain for cellulose bio-degradation utilization. |
| format | Article |
| id | doaj-art-6bee82e1d41d49afad2d4ebc94495ec7 |
| institution | DOAJ |
| issn | 0254-5071 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Editorial Department of China Brewing |
| record_format | Article |
| series | Zhongguo niangzao |
| spelling | doaj-art-6bee82e1d41d49afad2d4ebc94495ec72025-08-20T02:55:05ZengEditorial Department of China BrewingZhongguo niangzao0254-50712024-09-01439727810.11882/j.issn.0254-5071.2024.09.011Screening, identification and enzyme production conditions optimization of a cellulase-producing yeastRAN Guangyao, TANG Jiadai, ZHAO Yimei, MENG Zhuoni, LONG Yafei, GUO Min, GUO Ju0Department of Liquor Making Engineering, Moutai Institute, Zunyi 564500, ChinaIn order to screen high cellulose-producing yeast, cellulose-producing yeasts were screened from the fermented grains of sauce-flavor (Jiangxiangxing) Baijiu by Rose Bengal Agar and Congo Red Medium, and the screened strains were identified by morphological observation, physiological and biochemical tests, and molecular biology technique. The tolerance was investigated and the enzyme production conditions were optimized. The results showed that a cellulase-producing strain JXJP-Y19 was screened, and the strain JXJP-Y19 was identified as Yarrowia lipolytica. The temperature, ethanol, and pH tolerance of the strain JXJP-Y19 were 39 ℃, 8%(V/V), and 3, respectively, showing good tolerance to environmental stress. The optimal enzyme production conditions were as follows: bran as carbon source, cottonseed cake as nitrogen source, inoculum 1%, initial pH 6 and fermentation temperature 30 ℃. Under these optimal conditions, the activities of filter paper enzyme, exoglucanase and β-glucosidase produced by the strain JXJP-Y19 were (0.913±0.025) U/ml, (1.272±0.025) U/ml and (0.937±0.030) U/ml, respectively, which were 2.15 times, 1.81 times, and 1.94 times higher than before optimization. The strain JXJP-Y19 showed good cellulase activity and could be used as a potential strain for cellulose bio-degradation utilization.https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-9-72.pdfyeast|screening|identification|cellulase|enzyme production conditions optimization |
| spellingShingle | RAN Guangyao, TANG Jiadai, ZHAO Yimei, MENG Zhuoni, LONG Yafei, GUO Min, GUO Ju Screening, identification and enzyme production conditions optimization of a cellulase-producing yeast Zhongguo niangzao yeast|screening|identification|cellulase|enzyme production conditions optimization |
| title | Screening, identification and enzyme production conditions optimization of a cellulase-producing yeast |
| title_full | Screening, identification and enzyme production conditions optimization of a cellulase-producing yeast |
| title_fullStr | Screening, identification and enzyme production conditions optimization of a cellulase-producing yeast |
| title_full_unstemmed | Screening, identification and enzyme production conditions optimization of a cellulase-producing yeast |
| title_short | Screening, identification and enzyme production conditions optimization of a cellulase-producing yeast |
| title_sort | screening identification and enzyme production conditions optimization of a cellulase producing yeast |
| topic | yeast|screening|identification|cellulase|enzyme production conditions optimization |
| url | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-9-72.pdf |
| work_keys_str_mv | AT ranguangyaotangjiadaizhaoyimeimengzhuonilongyafeiguominguoju screeningidentificationandenzymeproductionconditionsoptimizationofacellulaseproducingyeast |