Characterization of uropathogenic ESBL-producing Escherichia coli isolated from hospitalized patients in western Algeria
Introduction: The aim of this study is to assess the prevalence and molecular characterization of uropathogenic Extended spectrum β-lactamases (ESBLs) producing Escherichia coli. Methodology: During 3 years, all hospitalized patients at the University-affiliated hospital of Tlemcen and presentin...
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The Journal of Infection in Developing Countries
2019-04-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/10702 |
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| author | Fatima Zenati Abouddihaj Barguigua Kaotar Nayme Fethi Benbelaïd Abdelmounaïm Khadir Chafika Bellahsene Mourad Bendahou Hassaïne Hafida Mohammed Timinouni |
| author_facet | Fatima Zenati Abouddihaj Barguigua Kaotar Nayme Fethi Benbelaïd Abdelmounaïm Khadir Chafika Bellahsene Mourad Bendahou Hassaïne Hafida Mohammed Timinouni |
| author_sort | Fatima Zenati |
| collection | DOAJ |
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Introduction: The aim of this study is to assess the prevalence and molecular characterization of uropathogenic Extended spectrum β-lactamases (ESBLs) producing Escherichia coli.
Methodology: During 3 years, all hospitalized patients at the University-affiliated hospital of Tlemcen and presenting urinary tract infections caused by E. coli were considered as potential study participants. These E. coli isolates were examined phenotypically for ESBL production. ESBL strains were subjected to antimicrobial susceptibility testing and were investigated for the presence of plasmid mediated quinolone resistance genes, 16SrRNA methylase genes and virulence genes by using conventional PCR and DNA sequencing. The molecular characterization of ESBL strains was established by phylogenetic grouping method and ERIC-PCR.
Results: The overall prevalence of ESBL was 32.5%. The blaCTX-M-15 was the most frequently detected in ESBL isolates, followed by blaCTX-M-14, blaCTX-M-28, blaCTX-M-1 and blaSHV-12 respectively. The plasmid-mediated quinolone resistance genes were detected in the 15 ESBL strains with the aac(6’)-Ib-cr gene was the most detected followed by qnrB1 and qnrA1 gene respectively. Among the 22 ESBL isolates resistant to gentamicin and amikacin, the 16SrRNA methylase genes were detected in 4 isolates. The sfa and pap virulent genes were founds in 26% and 22% of isolates receptively. The genotyping analysis of all strains revealed that almost were belonged to phylogenetic groups A1 and A0 and fourteen distinct clones.
Conclusion: The emergence of uropathogenic ESBL isolates and the high rate of blaCTX-M are alarming in Algeria. Strict measure must be required to control the further spread of these strains in Algerian hospitals.
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| format | Article |
| id | doaj-art-6be3d50008594fe7be3915fc2f1203b8 |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2019-04-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-6be3d50008594fe7be3915fc2f1203b82025-08-20T02:57:52ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802019-04-01130410.3855/jidc.10702Characterization of uropathogenic ESBL-producing Escherichia coli isolated from hospitalized patients in western AlgeriaFatima Zenati0Abouddihaj Barguigua1Kaotar Nayme2Fethi Benbelaïd3Abdelmounaïm Khadir4Chafika Bellahsene5Mourad Bendahou6Hassaïne Hafida7Mohammed Timinouni8Laboratory of Applied Microbiology in Food, Biomedical and Environment, Aboubekr Belkaïd University, Tlemcen, AlgeriaLaboratory of Biotechnology and Sustainable Development of Natural Resources, Polydisciplinary Faculty, Sultan Moulay Slimane University, Beni Mellal, MoroccoMolecular Bacteriology Laboratory, Pasteur Institute of Morocco, Casablanca, MoroccoLaboratory of Applied Microbiology in Food, Biomedical and Environment, Aboubekr Belkaïd University, Tlemcen, AlgeriaLaboratory of Applied Microbiology in Food, Biomedical and Environment, Aboubekr Belkaïd University, Tlemcen, AlgeriaLaboratory of Applied Microbiology in Food, Biomedical and Environment, Aboubekr Belkaïd University, Tlemcen, AlgeriaLaboratory of Applied Microbiology in Food, Biomedical and Environment, Aboubekr Belkaïd University, Tlemcen, AlgeriaLaboratory of Applied Microbiology in Food, Biomedical and Environment, Aboubekr Belkaïd University, Tlemcen, AlgeriaLaboratory of Applied Microbiology in Food, Biomedical and Environment, Aboubekr Belkaïd University, Tlemcen, Algeria Introduction: The aim of this study is to assess the prevalence and molecular characterization of uropathogenic Extended spectrum β-lactamases (ESBLs) producing Escherichia coli. Methodology: During 3 years, all hospitalized patients at the University-affiliated hospital of Tlemcen and presenting urinary tract infections caused by E. coli were considered as potential study participants. These E. coli isolates were examined phenotypically for ESBL production. ESBL strains were subjected to antimicrobial susceptibility testing and were investigated for the presence of plasmid mediated quinolone resistance genes, 16SrRNA methylase genes and virulence genes by using conventional PCR and DNA sequencing. The molecular characterization of ESBL strains was established by phylogenetic grouping method and ERIC-PCR. Results: The overall prevalence of ESBL was 32.5%. The blaCTX-M-15 was the most frequently detected in ESBL isolates, followed by blaCTX-M-14, blaCTX-M-28, blaCTX-M-1 and blaSHV-12 respectively. The plasmid-mediated quinolone resistance genes were detected in the 15 ESBL strains with the aac(6’)-Ib-cr gene was the most detected followed by qnrB1 and qnrA1 gene respectively. Among the 22 ESBL isolates resistant to gentamicin and amikacin, the 16SrRNA methylase genes were detected in 4 isolates. The sfa and pap virulent genes were founds in 26% and 22% of isolates receptively. The genotyping analysis of all strains revealed that almost were belonged to phylogenetic groups A1 and A0 and fourteen distinct clones. Conclusion: The emergence of uropathogenic ESBL isolates and the high rate of blaCTX-M are alarming in Algeria. Strict measure must be required to control the further spread of these strains in Algerian hospitals. https://jidc.org/index.php/journal/article/view/10702Extended-spectrum β-lactamaseUrinary tract infectionsEscherichia coli |
| spellingShingle | Fatima Zenati Abouddihaj Barguigua Kaotar Nayme Fethi Benbelaïd Abdelmounaïm Khadir Chafika Bellahsene Mourad Bendahou Hassaïne Hafida Mohammed Timinouni Characterization of uropathogenic ESBL-producing Escherichia coli isolated from hospitalized patients in western Algeria Journal of Infection in Developing Countries Extended-spectrum β-lactamase Urinary tract infections Escherichia coli |
| title | Characterization of uropathogenic ESBL-producing Escherichia coli isolated from hospitalized patients in western Algeria |
| title_full | Characterization of uropathogenic ESBL-producing Escherichia coli isolated from hospitalized patients in western Algeria |
| title_fullStr | Characterization of uropathogenic ESBL-producing Escherichia coli isolated from hospitalized patients in western Algeria |
| title_full_unstemmed | Characterization of uropathogenic ESBL-producing Escherichia coli isolated from hospitalized patients in western Algeria |
| title_short | Characterization of uropathogenic ESBL-producing Escherichia coli isolated from hospitalized patients in western Algeria |
| title_sort | characterization of uropathogenic esbl producing escherichia coli isolated from hospitalized patients in western algeria |
| topic | Extended-spectrum β-lactamase Urinary tract infections Escherichia coli |
| url | https://jidc.org/index.php/journal/article/view/10702 |
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