Effects of Brown Algae (<i>Laminaria japonica</i>) Extract on Growth Performance, Immune Function and Intestinal Health of Largemouth Bass (<i>Micropterus salmoides</i>)

This study used largemouth bass (initial average weight: 33.33 ± 1.8 g) to explore the effects of adding different brown algae extracts to feed on the fish’s growth, immunity and intestinal health. Six groups were set up: a control (Group A), 0.1% sodium alginate (Group B), 0.1% oligotriosaccharide...

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Main Authors: Jiajia Shen, Hongxiang Liu, Mengran Wang, Bo Lu, Ke Ke, Yunyong Wei, Feng Gao, Qiaozhen Wang, Shushi Huang, Yanqun Ma
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/15/5/622
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author Jiajia Shen
Hongxiang Liu
Mengran Wang
Bo Lu
Ke Ke
Yunyong Wei
Feng Gao
Qiaozhen Wang
Shushi Huang
Yanqun Ma
author_facet Jiajia Shen
Hongxiang Liu
Mengran Wang
Bo Lu
Ke Ke
Yunyong Wei
Feng Gao
Qiaozhen Wang
Shushi Huang
Yanqun Ma
author_sort Jiajia Shen
collection DOAJ
description This study used largemouth bass (initial average weight: 33.33 ± 1.8 g) to explore the effects of adding different brown algae extracts to feed on the fish’s growth, immunity and intestinal health. Six groups were set up: a control (Group A), 0.1% sodium alginate (Group B), 0.1% oligotriosaccharide I (Group C), 0.1% oligotriosaccharide II (Group D), 0.2% brown algae powder (Group E) and 0.2% brown algae powder enzymatic product (Group F), with three replicates of 35 fish each, and a 56-day feeding experiment. Results: Compared to Group A, Groups C, D and F had a higher specific growth rate and lower feed coefficient (<i>p</i> < 0.05). Group D had enhanced serum SOD activity; Group F had increased antioxidant enzyme activity and decreased MDA content (<i>p</i> < 0.05). All experimental groups had higher serum LZM levels (<i>p</i> < 0.05), with no IgM difference (<i>p</i> > 0.05). In the intestine, treatment groups had higher α-amylase activity (<i>p</i> < 0.05) and no lipase difference (<i>p</i> > 0.05), and Groups C, D and F had higher trypsin activity (<i>p</i> < 0.05). Group F had the tallest villi, Group B had the thickest muscular layer (<i>p</i> < 0.05), and villus width was similar among groups (<i>p</i> > 0.05). The experimental groups had fewer intestinal pathogenic bacteria, and Group F had improved intestinal microorganism diversity and richness (<i>p</i> < 0.05). In conclusion, adding 0.1% oligotriosaccharide and 0.2% brown algae powder enzymatic product to feed can promote largemouth bass growth, antioxidant capacity and immunity. The 0.2% brown algae powder enzymatic product is better for intestinal development and flora improvement.
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spelling doaj-art-6bd9e7a1c8fa43d58b80538bd10fc3ac2025-08-20T02:52:48ZengMDPI AGAnimals2076-26152025-02-0115562210.3390/ani15050622Effects of Brown Algae (<i>Laminaria japonica</i>) Extract on Growth Performance, Immune Function and Intestinal Health of Largemouth Bass (<i>Micropterus salmoides</i>)Jiajia Shen0Hongxiang Liu1Mengran Wang2Bo Lu3Ke Ke4Yunyong Wei5Feng Gao6Qiaozhen Wang7Shushi Huang8Yanqun Ma9Key Laboratory of Aquatic Healthy Breeding and Nutrition Regulation of Guangxi Universities, College of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaKey Laboratory of Aquatic Healthy Breeding and Nutrition Regulation of Guangxi Universities, College of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaKey Laboratory of Aquatic Healthy Breeding and Nutrition Regulation of Guangxi Universities, College of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaGuangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynethesis Chemistry, Guangxi Academy of Sciences, Nanning 530007, ChinaGuangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynethesis Chemistry, Guangxi Academy of Sciences, Nanning 530007, ChinaKey Laboratory of Aquatic Healthy Breeding and Nutrition Regulation of Guangxi Universities, College of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaKey Laboratory of Aquatic Healthy Breeding and Nutrition Regulation of Guangxi Universities, College of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaNational Key Laboratory of Non-Food Biomass Energy Technology, National Engineering Research Center for Non-Food Biorefinery, Institute of Biological Science and Technology, Guangxi Academy of Sciences, Nanning 530007, ChinaGuangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynethesis Chemistry, Guangxi Academy of Sciences, Nanning 530007, ChinaKey Laboratory of Aquatic Healthy Breeding and Nutrition Regulation of Guangxi Universities, College of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaThis study used largemouth bass (initial average weight: 33.33 ± 1.8 g) to explore the effects of adding different brown algae extracts to feed on the fish’s growth, immunity and intestinal health. Six groups were set up: a control (Group A), 0.1% sodium alginate (Group B), 0.1% oligotriosaccharide I (Group C), 0.1% oligotriosaccharide II (Group D), 0.2% brown algae powder (Group E) and 0.2% brown algae powder enzymatic product (Group F), with three replicates of 35 fish each, and a 56-day feeding experiment. Results: Compared to Group A, Groups C, D and F had a higher specific growth rate and lower feed coefficient (<i>p</i> < 0.05). Group D had enhanced serum SOD activity; Group F had increased antioxidant enzyme activity and decreased MDA content (<i>p</i> < 0.05). All experimental groups had higher serum LZM levels (<i>p</i> < 0.05), with no IgM difference (<i>p</i> > 0.05). In the intestine, treatment groups had higher α-amylase activity (<i>p</i> < 0.05) and no lipase difference (<i>p</i> > 0.05), and Groups C, D and F had higher trypsin activity (<i>p</i> < 0.05). Group F had the tallest villi, Group B had the thickest muscular layer (<i>p</i> < 0.05), and villus width was similar among groups (<i>p</i> > 0.05). The experimental groups had fewer intestinal pathogenic bacteria, and Group F had improved intestinal microorganism diversity and richness (<i>p</i> < 0.05). In conclusion, adding 0.1% oligotriosaccharide and 0.2% brown algae powder enzymatic product to feed can promote largemouth bass growth, antioxidant capacity and immunity. The 0.2% brown algae powder enzymatic product is better for intestinal development and flora improvement.https://www.mdpi.com/2076-2615/15/5/622<i>Micropterus salmoides</i>brown algae extractgrowthintestinal flora
spellingShingle Jiajia Shen
Hongxiang Liu
Mengran Wang
Bo Lu
Ke Ke
Yunyong Wei
Feng Gao
Qiaozhen Wang
Shushi Huang
Yanqun Ma
Effects of Brown Algae (<i>Laminaria japonica</i>) Extract on Growth Performance, Immune Function and Intestinal Health of Largemouth Bass (<i>Micropterus salmoides</i>)
Animals
<i>Micropterus salmoides</i>
brown algae extract
growth
intestinal flora
title Effects of Brown Algae (<i>Laminaria japonica</i>) Extract on Growth Performance, Immune Function and Intestinal Health of Largemouth Bass (<i>Micropterus salmoides</i>)
title_full Effects of Brown Algae (<i>Laminaria japonica</i>) Extract on Growth Performance, Immune Function and Intestinal Health of Largemouth Bass (<i>Micropterus salmoides</i>)
title_fullStr Effects of Brown Algae (<i>Laminaria japonica</i>) Extract on Growth Performance, Immune Function and Intestinal Health of Largemouth Bass (<i>Micropterus salmoides</i>)
title_full_unstemmed Effects of Brown Algae (<i>Laminaria japonica</i>) Extract on Growth Performance, Immune Function and Intestinal Health of Largemouth Bass (<i>Micropterus salmoides</i>)
title_short Effects of Brown Algae (<i>Laminaria japonica</i>) Extract on Growth Performance, Immune Function and Intestinal Health of Largemouth Bass (<i>Micropterus salmoides</i>)
title_sort effects of brown algae i laminaria japonica i extract on growth performance immune function and intestinal health of largemouth bass i micropterus salmoides i
topic <i>Micropterus salmoides</i>
brown algae extract
growth
intestinal flora
url https://www.mdpi.com/2076-2615/15/5/622
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