Effects of Brown Algae (<i>Laminaria japonica</i>) Extract on Growth Performance, Immune Function and Intestinal Health of Largemouth Bass (<i>Micropterus salmoides</i>)

This study used largemouth bass (initial average weight: 33.33 ± 1.8 g) to explore the effects of adding different brown algae extracts to feed on the fish’s growth, immunity and intestinal health. Six groups were set up: a control (Group A), 0.1% sodium alginate (Group B), 0.1% oligotriosaccharide...

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Main Authors: Jiajia Shen, Hongxiang Liu, Mengran Wang, Bo Lu, Ke Ke, Yunyong Wei, Feng Gao, Qiaozhen Wang, Shushi Huang, Yanqun Ma
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/15/5/622
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Summary:This study used largemouth bass (initial average weight: 33.33 ± 1.8 g) to explore the effects of adding different brown algae extracts to feed on the fish’s growth, immunity and intestinal health. Six groups were set up: a control (Group A), 0.1% sodium alginate (Group B), 0.1% oligotriosaccharide I (Group C), 0.1% oligotriosaccharide II (Group D), 0.2% brown algae powder (Group E) and 0.2% brown algae powder enzymatic product (Group F), with three replicates of 35 fish each, and a 56-day feeding experiment. Results: Compared to Group A, Groups C, D and F had a higher specific growth rate and lower feed coefficient (<i>p</i> < 0.05). Group D had enhanced serum SOD activity; Group F had increased antioxidant enzyme activity and decreased MDA content (<i>p</i> < 0.05). All experimental groups had higher serum LZM levels (<i>p</i> < 0.05), with no IgM difference (<i>p</i> > 0.05). In the intestine, treatment groups had higher α-amylase activity (<i>p</i> < 0.05) and no lipase difference (<i>p</i> > 0.05), and Groups C, D and F had higher trypsin activity (<i>p</i> < 0.05). Group F had the tallest villi, Group B had the thickest muscular layer (<i>p</i> < 0.05), and villus width was similar among groups (<i>p</i> > 0.05). The experimental groups had fewer intestinal pathogenic bacteria, and Group F had improved intestinal microorganism diversity and richness (<i>p</i> < 0.05). In conclusion, adding 0.1% oligotriosaccharide and 0.2% brown algae powder enzymatic product to feed can promote largemouth bass growth, antioxidant capacity and immunity. The 0.2% brown algae powder enzymatic product is better for intestinal development and flora improvement.
ISSN:2076-2615