Effects of Hyaluronic Acid on Three Different Cell Types of the Periodontium in a Novel Multi-Culture Cell Plate: An Exploratory Study

Hyaluronic acid (HA) has received considerable attention in the reconstruction of lost periodontal tissues. HA has been proposed to play a role in cell proliferation, differentiation, migration, and cell–matrix as well as cell–cell interactions. Although various studies have been conducted, further...

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Main Authors: Alessio Barbieri, Luciano Pitzurra, Bruno Loos, Ineke D. C. Jansen
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Biomolecules
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Online Access:https://www.mdpi.com/2218-273X/15/1/152
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author Alessio Barbieri
Luciano Pitzurra
Bruno Loos
Ineke D. C. Jansen
author_facet Alessio Barbieri
Luciano Pitzurra
Bruno Loos
Ineke D. C. Jansen
author_sort Alessio Barbieri
collection DOAJ
description Hyaluronic acid (HA) has received considerable attention in the reconstruction of lost periodontal tissues. HA has been proposed to play a role in cell proliferation, differentiation, migration, and cell–matrix as well as cell–cell interactions. Although various studies have been conducted, further research is needed to expand our knowledge based on HA such as its effects on cell proliferation and osteogenic differentiation. The aim of this study is to assess, in single- and multi-culture plate models, the effect of HA on the proliferation, viability, and function of periodontal ligament fibroblasts, osteoblasts, and gingival epithelial cells. A novel multi-culture cell plate was chosen to simulate a cell–cell communication as close as possible to a real clinical condition in an in vitro setting. We found that HA exclusively enhanced epithelial cell proliferation, while intercellular communication stimulated the proliferation and osteogenic potential of the osteoblasts, independently from HA use. The proliferation and function of the periodontal ligament fibroblasts were not changed by HA or the cellular interplay. The use of multi-culture plates could represent a promising method to investigate and compare dental biomaterials in experiments mimicking an in vivo environment.
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spelling doaj-art-6bc153a59de64eb8958aa54aa731d7b92025-01-24T13:25:23ZengMDPI AGBiomolecules2218-273X2025-01-0115115210.3390/biom15010152Effects of Hyaluronic Acid on Three Different Cell Types of the Periodontium in a Novel Multi-Culture Cell Plate: An Exploratory StudyAlessio Barbieri0Luciano Pitzurra1Bruno Loos2Ineke D. C. Jansen3Department of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, 1081 LA Amsterdam, The NetherlandsDepartment of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, 1081 LA Amsterdam, The NetherlandsDepartment of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, 1081 LA Amsterdam, The NetherlandsDepartment of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, 1081 LA Amsterdam, The NetherlandsHyaluronic acid (HA) has received considerable attention in the reconstruction of lost periodontal tissues. HA has been proposed to play a role in cell proliferation, differentiation, migration, and cell–matrix as well as cell–cell interactions. Although various studies have been conducted, further research is needed to expand our knowledge based on HA such as its effects on cell proliferation and osteogenic differentiation. The aim of this study is to assess, in single- and multi-culture plate models, the effect of HA on the proliferation, viability, and function of periodontal ligament fibroblasts, osteoblasts, and gingival epithelial cells. A novel multi-culture cell plate was chosen to simulate a cell–cell communication as close as possible to a real clinical condition in an in vitro setting. We found that HA exclusively enhanced epithelial cell proliferation, while intercellular communication stimulated the proliferation and osteogenic potential of the osteoblasts, independently from HA use. The proliferation and function of the periodontal ligament fibroblasts were not changed by HA or the cellular interplay. The use of multi-culture plates could represent a promising method to investigate and compare dental biomaterials in experiments mimicking an in vivo environment.https://www.mdpi.com/2218-273X/15/1/152hyaluronic acidperiodontal cellsin vivo studyin vitro study
spellingShingle Alessio Barbieri
Luciano Pitzurra
Bruno Loos
Ineke D. C. Jansen
Effects of Hyaluronic Acid on Three Different Cell Types of the Periodontium in a Novel Multi-Culture Cell Plate: An Exploratory Study
Biomolecules
hyaluronic acid
periodontal cells
in vivo study
in vitro study
title Effects of Hyaluronic Acid on Three Different Cell Types of the Periodontium in a Novel Multi-Culture Cell Plate: An Exploratory Study
title_full Effects of Hyaluronic Acid on Three Different Cell Types of the Periodontium in a Novel Multi-Culture Cell Plate: An Exploratory Study
title_fullStr Effects of Hyaluronic Acid on Three Different Cell Types of the Periodontium in a Novel Multi-Culture Cell Plate: An Exploratory Study
title_full_unstemmed Effects of Hyaluronic Acid on Three Different Cell Types of the Periodontium in a Novel Multi-Culture Cell Plate: An Exploratory Study
title_short Effects of Hyaluronic Acid on Three Different Cell Types of the Periodontium in a Novel Multi-Culture Cell Plate: An Exploratory Study
title_sort effects of hyaluronic acid on three different cell types of the periodontium in a novel multi culture cell plate an exploratory study
topic hyaluronic acid
periodontal cells
in vivo study
in vitro study
url https://www.mdpi.com/2218-273X/15/1/152
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AT brunoloos effectsofhyaluronicacidonthreedifferentcelltypesoftheperiodontiuminanovelmulticulturecellplateanexploratorystudy
AT inekedcjansen effectsofhyaluronicacidonthreedifferentcelltypesoftheperiodontiuminanovelmulticulturecellplateanexploratorystudy