Immunomodulatory Properties of Multi-Strain Postbiotics on Human CD14<sup>+</sup> Monocytes
The ability of probiotics, comprising live microbiota, to modulate the composition of intestinal microbiomes has been connected to modulation of the central nervous system (Gut–Brain axis), neuroendocrine system (Gut–Skin axis), and immune response (Gut–Immune axis). Less information is known regard...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
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| Series: | Life |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-1729/14/12/1673 |
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| Summary: | The ability of probiotics, comprising live microbiota, to modulate the composition of intestinal microbiomes has been connected to modulation of the central nervous system (Gut–Brain axis), neuroendocrine system (Gut–Skin axis), and immune response (Gut–Immune axis). Less information is known regarding the ability of postbiotics (cell wall components and secreted metabolites derived from live organisms) to regulate host immunity. In the present study, we tested postbiotics comprising single strains of bacteria and yeast (<i>Lactobacillus acidophilus</i> 16axg, <i>Lacticaseibacillus rhamnosus</i> 18fx, <i>Saccharomyces cerevisiae var. boulardii</i> 16mxg) as well as combinations of multiple strains for their ability to stimulate cytokine production by human CD14<sup>+</sup> monocytes. We quantified cytokine gene and protein expression levels in monocytes following stimulation with postbiotics. Both heat-killed <i>L. acidophilus</i> and <i>L. rhamnosus</i> stimulated naïve monocytes without significant differences between them. Heat-killed <i>S. boulardii</i> stimulated less cytokine production compared to postbiotic bacteria at the same concentration. Interestingly, the addition of heat-killed yeast to heat-killed <i>L. acidophilus</i> and <i>L. rhamnosus</i> resulted in an enhancement of immune stimulation. Thus, heat-killed postbiotics have immune-modulating potential, particularly when bacteria and yeast are combined. This approach may hold promise for developing targeted interventions that can be fine-tuned to modulate host immune response with beneficial health impact. |
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| ISSN: | 2075-1729 |