Levofloxacin loaded PMMA:PVP blended nanofiber mat as an antibacterial material
Polymeric nanofibers loaded with antibacterial agents occupy a leading position among many technologies for producing antibacterial materials due to their unique properties. In this work, a novel electrospun nanofiber mat loaded with antibacterial antibiotic levofloxacin (LV) was developed using a b...
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| Format: | Article |
| Language: | English |
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IOP Publishing
2025-01-01
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| Series: | Materials Research Express |
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| Online Access: | https://doi.org/10.1088/2053-1591/adec42 |
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| author | V A Karachevtsev A M Plokhotnichenko O V Trufanov S G Stepanian |
| author_facet | V A Karachevtsev A M Plokhotnichenko O V Trufanov S G Stepanian |
| author_sort | V A Karachevtsev |
| collection | DOAJ |
| description | Polymeric nanofibers loaded with antibacterial agents occupy a leading position among many technologies for producing antibacterial materials due to their unique properties. In this work, a novel electrospun nanofiber mat loaded with antibacterial antibiotic levofloxacin (LV) was developed using a blend of hydrophilic (polyvinylpyrrolidone (PVP)) and hydrophobic (polymethyl methacrylate (PMMA)) polymers. The diameter of the obtained nanofibers is in the range of 1–4 μm. The release of LV from the nanofiber mat after soaking in water was studied by UV–vis absorption spectroscopy. PVP facilitates the incorporation of antibacterial agents into the nanofibers and promotes their release in the aqueous solution. From the temporal dependence of LV release from PMMA:PVP blend nanofibers follows that about half of the LV is released into aqueous solution during the first 5 min while the other 1/4 of the LV is released during the next 2–3 h. A complete release of LV from PMMA nanofibers is not observed because a certain part of the nanopores is closed. The formation of non-covalent complexes between LV and PVP was examined within the framework of quantum chemical theory. A tetrameric model of vinylpyrrolidone was employed to simulate interactions with LV. The most stable complex was identified, with an interaction energy of –58.9 kJ mol ^−1 . These findings suggest that such molecular complexation may have an impact on the drug release behavior. In the nanofiber mat, PMMA promotes the gradual release of antibacterial drug, and does not degrade upon contact with water and/or with microorganisms. The PMMA:PVP blended nanofiber mat can serve as an effective delivery system for the antibiotic LV, maintaining its bactericidal effects, as evidenced by the growth inhibition of both Gram-positive and Gram-negative bacteria. It was found that the LV released from the nanofiber mat more strongly inhibited the growth of E. coli B compared to S. aureus and P. aeruginosa . |
| format | Article |
| id | doaj-art-6bbce0dfcecd4ab2bc92a5364113f380 |
| institution | OA Journals |
| issn | 2053-1591 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | IOP Publishing |
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| series | Materials Research Express |
| spelling | doaj-art-6bbce0dfcecd4ab2bc92a5364113f3802025-08-20T02:37:16ZengIOP PublishingMaterials Research Express2053-15912025-01-0112707540310.1088/2053-1591/adec42Levofloxacin loaded PMMA:PVP blended nanofiber mat as an antibacterial materialV A Karachevtsev0https://orcid.org/0000-0003-4580-6465A M Plokhotnichenko1O V Trufanov2https://orcid.org/0000-0002-3978-3335S G Stepanian3https://orcid.org/0000-0003-0899-0320B Verkin Institute for Low Temperature Physics and Engineering of National Academy of Sciences of Ukraine, 47 Nauky Ave, Kharkiv, 61103, UkraineB Verkin Institute for Low Temperature Physics and Engineering of National Academy of Sciences of Ukraine, 47 Nauky Ave, Kharkiv, 61103, UkraineInstitute for Problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine, 23 Pereyaslavska Street, Kharkiv, 61016, UkraineB Verkin Institute for Low Temperature Physics and Engineering of National Academy of Sciences of Ukraine, 47 Nauky Ave, Kharkiv, 61103, UkrainePolymeric nanofibers loaded with antibacterial agents occupy a leading position among many technologies for producing antibacterial materials due to their unique properties. In this work, a novel electrospun nanofiber mat loaded with antibacterial antibiotic levofloxacin (LV) was developed using a blend of hydrophilic (polyvinylpyrrolidone (PVP)) and hydrophobic (polymethyl methacrylate (PMMA)) polymers. The diameter of the obtained nanofibers is in the range of 1–4 μm. The release of LV from the nanofiber mat after soaking in water was studied by UV–vis absorption spectroscopy. PVP facilitates the incorporation of antibacterial agents into the nanofibers and promotes their release in the aqueous solution. From the temporal dependence of LV release from PMMA:PVP blend nanofibers follows that about half of the LV is released into aqueous solution during the first 5 min while the other 1/4 of the LV is released during the next 2–3 h. A complete release of LV from PMMA nanofibers is not observed because a certain part of the nanopores is closed. The formation of non-covalent complexes between LV and PVP was examined within the framework of quantum chemical theory. A tetrameric model of vinylpyrrolidone was employed to simulate interactions with LV. The most stable complex was identified, with an interaction energy of –58.9 kJ mol ^−1 . These findings suggest that such molecular complexation may have an impact on the drug release behavior. In the nanofiber mat, PMMA promotes the gradual release of antibacterial drug, and does not degrade upon contact with water and/or with microorganisms. The PMMA:PVP blended nanofiber mat can serve as an effective delivery system for the antibiotic LV, maintaining its bactericidal effects, as evidenced by the growth inhibition of both Gram-positive and Gram-negative bacteria. It was found that the LV released from the nanofiber mat more strongly inhibited the growth of E. coli B compared to S. aureus and P. aeruginosa .https://doi.org/10.1088/2053-1591/adec42nanofibersantibacteriallevofloxacinGram-positive bacteriaGram-negative bacteriaPMMA |
| spellingShingle | V A Karachevtsev A M Plokhotnichenko O V Trufanov S G Stepanian Levofloxacin loaded PMMA:PVP blended nanofiber mat as an antibacterial material Materials Research Express nanofibers antibacterial levofloxacin Gram-positive bacteria Gram-negative bacteria PMMA |
| title | Levofloxacin loaded PMMA:PVP blended nanofiber mat as an antibacterial material |
| title_full | Levofloxacin loaded PMMA:PVP blended nanofiber mat as an antibacterial material |
| title_fullStr | Levofloxacin loaded PMMA:PVP blended nanofiber mat as an antibacterial material |
| title_full_unstemmed | Levofloxacin loaded PMMA:PVP blended nanofiber mat as an antibacterial material |
| title_short | Levofloxacin loaded PMMA:PVP blended nanofiber mat as an antibacterial material |
| title_sort | levofloxacin loaded pmma pvp blended nanofiber mat as an antibacterial material |
| topic | nanofibers antibacterial levofloxacin Gram-positive bacteria Gram-negative bacteria PMMA |
| url | https://doi.org/10.1088/2053-1591/adec42 |
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