Exploring the Potential of Enhanced Prognostic Performance of NCCN‐IPI in Diffuse Large B‐Cell Lymphoma by Integrating Tumor Microenvironment Markers: Stromal FOXC1 and Tumor pERK1/2 Expression

ABSTRACT Background FOXC1 and ERK1‐2 are proteins implicated in aggressive biological behavior of various malignancies including lymphomas. Material and Methods We investigate the additive prognostic value of stromal FOXC1 expression and tumor phosphorylated ERK1‐2 (pERK1‐2) expression to the establ...

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Main Authors: Ji‐Ye Kim, Ibadullah Kahttana, Hyonok Yoon, Sunhee Chang, Sun Och Yoon
Format: Article
Language:English
Published: Wiley 2024-10-01
Series:Cancer Medicine
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Online Access:https://doi.org/10.1002/cam4.70305
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author Ji‐Ye Kim
Ibadullah Kahttana
Hyonok Yoon
Sunhee Chang
Sun Och Yoon
author_facet Ji‐Ye Kim
Ibadullah Kahttana
Hyonok Yoon
Sunhee Chang
Sun Och Yoon
author_sort Ji‐Ye Kim
collection DOAJ
description ABSTRACT Background FOXC1 and ERK1‐2 are proteins implicated in aggressive biological behavior of various malignancies including lymphomas. Material and Methods We investigate the additive prognostic value of stromal FOXC1 expression and tumor phosphorylated ERK1‐2 (pERK1‐2) expression to the established National Comprehensive Cancer Network International Prognostic Index (NCCN‐IPI), in 92 diffuse large B‐cell lymphoma (DLBCL) cases. Multidimensional analysis using statistics and machine learning (ML) models assessed prognostic value of established clinicopathologic variables with stromal FOXC1 and tumor pERK1‐2 expressions. Results Both high FOXC1 stroma group and high pERK1‐2 tumor group were significantly associated with shorter progression‐free survival (PFS) and overall survival (OS) compared with low group (p = 0.015, 0.034 and p = 0.025, 0.025 each respectively). In multivariable analysis, high FOXC1 stromal expression was an independent prognostic factor of OS (p = 0.037). The addition of stromal FOXC1 and tumor pERK1‐2 to the NCCN‐IPI score significantly improved prediction of time to death compared with NCCN‐IPI score alone (Harrell's C‐index = 0.801 vs. 0.764; p = 0.030). ML models reconfirmed the addition of stromal FOXC1 expression and tumor pERK1‐2 to NCCN‐IPI score had the highest C‐index (0.952) among combinations. Stromal FOXC1 and tumor pERK1‐2 were determinants of DLBCL prognosis, whose addition significantly improved prognostic performance of the NCCN‐IPI.
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spelling doaj-art-6bbc5b62926341b3bd2e21fc42b84abb2025-08-20T03:07:58ZengWileyCancer Medicine2045-76342024-10-011319n/an/a10.1002/cam4.70305Exploring the Potential of Enhanced Prognostic Performance of NCCN‐IPI in Diffuse Large B‐Cell Lymphoma by Integrating Tumor Microenvironment Markers: Stromal FOXC1 and Tumor pERK1/2 ExpressionJi‐Ye Kim0Ibadullah Kahttana1Hyonok Yoon2Sunhee Chang3Sun Och Yoon4Department of Pathology, Ilsan Paik Hospital Inje University College of Medicine Goyang‐si Gyeonggi‐do Republic of KoreaDivision of Electronics and Information Engineering Jeonbuk National University Jeonju‐si Republic of KoreaCollege of Pharmacy Research Institute of Pharmaceutical Sciences, Gyeongsang National University Jinju‐si Republic of KoreaDepartment of Pathology, Ilsan Paik Hospital Inje University College of Medicine Goyang‐si Gyeonggi‐do Republic of KoreaDepartment of Pathology Yonsei University College of Medicine, Severance Hospital Seoul Republic of KoreaABSTRACT Background FOXC1 and ERK1‐2 are proteins implicated in aggressive biological behavior of various malignancies including lymphomas. Material and Methods We investigate the additive prognostic value of stromal FOXC1 expression and tumor phosphorylated ERK1‐2 (pERK1‐2) expression to the established National Comprehensive Cancer Network International Prognostic Index (NCCN‐IPI), in 92 diffuse large B‐cell lymphoma (DLBCL) cases. Multidimensional analysis using statistics and machine learning (ML) models assessed prognostic value of established clinicopathologic variables with stromal FOXC1 and tumor pERK1‐2 expressions. Results Both high FOXC1 stroma group and high pERK1‐2 tumor group were significantly associated with shorter progression‐free survival (PFS) and overall survival (OS) compared with low group (p = 0.015, 0.034 and p = 0.025, 0.025 each respectively). In multivariable analysis, high FOXC1 stromal expression was an independent prognostic factor of OS (p = 0.037). The addition of stromal FOXC1 and tumor pERK1‐2 to the NCCN‐IPI score significantly improved prediction of time to death compared with NCCN‐IPI score alone (Harrell's C‐index = 0.801 vs. 0.764; p = 0.030). ML models reconfirmed the addition of stromal FOXC1 expression and tumor pERK1‐2 to NCCN‐IPI score had the highest C‐index (0.952) among combinations. Stromal FOXC1 and tumor pERK1‐2 were determinants of DLBCL prognosis, whose addition significantly improved prognostic performance of the NCCN‐IPI.https://doi.org/10.1002/cam4.70305Diffuse Large B‐cell LymphomaFOXC1Machine Learning ModelspERK1‐2Prognostic MarkersTumor Microenvironment
spellingShingle Ji‐Ye Kim
Ibadullah Kahttana
Hyonok Yoon
Sunhee Chang
Sun Och Yoon
Exploring the Potential of Enhanced Prognostic Performance of NCCN‐IPI in Diffuse Large B‐Cell Lymphoma by Integrating Tumor Microenvironment Markers: Stromal FOXC1 and Tumor pERK1/2 Expression
Cancer Medicine
Diffuse Large B‐cell Lymphoma
FOXC1
Machine Learning Models
pERK1‐2
Prognostic Markers
Tumor Microenvironment
title Exploring the Potential of Enhanced Prognostic Performance of NCCN‐IPI in Diffuse Large B‐Cell Lymphoma by Integrating Tumor Microenvironment Markers: Stromal FOXC1 and Tumor pERK1/2 Expression
title_full Exploring the Potential of Enhanced Prognostic Performance of NCCN‐IPI in Diffuse Large B‐Cell Lymphoma by Integrating Tumor Microenvironment Markers: Stromal FOXC1 and Tumor pERK1/2 Expression
title_fullStr Exploring the Potential of Enhanced Prognostic Performance of NCCN‐IPI in Diffuse Large B‐Cell Lymphoma by Integrating Tumor Microenvironment Markers: Stromal FOXC1 and Tumor pERK1/2 Expression
title_full_unstemmed Exploring the Potential of Enhanced Prognostic Performance of NCCN‐IPI in Diffuse Large B‐Cell Lymphoma by Integrating Tumor Microenvironment Markers: Stromal FOXC1 and Tumor pERK1/2 Expression
title_short Exploring the Potential of Enhanced Prognostic Performance of NCCN‐IPI in Diffuse Large B‐Cell Lymphoma by Integrating Tumor Microenvironment Markers: Stromal FOXC1 and Tumor pERK1/2 Expression
title_sort exploring the potential of enhanced prognostic performance of nccn ipi in diffuse large b cell lymphoma by integrating tumor microenvironment markers stromal foxc1 and tumor perk1 2 expression
topic Diffuse Large B‐cell Lymphoma
FOXC1
Machine Learning Models
pERK1‐2
Prognostic Markers
Tumor Microenvironment
url https://doi.org/10.1002/cam4.70305
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