Extraction of kaempferol from Wattakaka volubilis and evaluation of its protective effects against aluminium-induced liver toxicity
Introduction: The goal of this study was to find out if kaempferol, a compound isolated from the Wattakaka volubilis plant, could protect the liver of rats that were given aluminum sulfate. Methods: Kaempferol, a bioflavonoid, was isolated from the leaves of W. volubilis methanolic extract (MEWV) an...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-08-01
|
| Series: | Phytomedicine Plus |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266703132500082X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849232624634036224 |
|---|---|
| author | Usharani Saminathan Anuradha Ramamoorthy Ambiga Somasundaram Sivasankari Sekar Sivagurunathan Paramasivam Pasiyappazham Ramasamy |
| author_facet | Usharani Saminathan Anuradha Ramamoorthy Ambiga Somasundaram Sivasankari Sekar Sivagurunathan Paramasivam Pasiyappazham Ramasamy |
| author_sort | Usharani Saminathan |
| collection | DOAJ |
| description | Introduction: The goal of this study was to find out if kaempferol, a compound isolated from the Wattakaka volubilis plant, could protect the liver of rats that were given aluminum sulfate. Methods: Kaempferol, a bioflavonoid, was isolated from the leaves of W. volubilis methanolic extract (MEWV) and characterized by using the High-performance thin-layer chromatography (HPTLC)technique. The liver protective activities of MEWV against aluminum poisoning were investigated in male albino rats, as their protective efficacy was assessed in addition to that of the silymarin standard. The oral administration of silymarin (25 mg/kg), kaempferol (10 mg/kg), and MEWV (200 mg/kg) was administered continuously for 14 days. The body and liver weights as well as serum enzymes like alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were calculated. Results: Advanced techniques successfully isolated and confirmed the bioactive constituent kaempferol from MEWV. When kaempferol and MEWV were given to rats that had been poisoned with aluminum, their body mass index went up significantly (p < 0.001) and the relative weight of their liver and serum marker enzymes went down significantly (p < 0.001) compared to animals that had only been poisoned and analyzed the comparative action of the extract and isolated compound using silymarin, the standard drug. Kaempferol showed better protection against aluminum-induced hepatotoxicity compared to MEWV treatment. Conclusion: In conclusion, the results showed that kaempferol and MEWV reduced the damage done to the liver by aluminum sulfate by protecting cells, the liver, and protecting antioxidants. |
| format | Article |
| id | doaj-art-6bb9fd06bd784290bb12dd0c719f7280 |
| institution | Kabale University |
| issn | 2667-0313 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Phytomedicine Plus |
| spelling | doaj-art-6bb9fd06bd784290bb12dd0c719f72802025-08-21T04:17:24ZengElsevierPhytomedicine Plus2667-03132025-08-015310081010.1016/j.phyplu.2025.100810Extraction of kaempferol from Wattakaka volubilis and evaluation of its protective effects against aluminium-induced liver toxicityUsharani Saminathan0Anuradha Ramamoorthy1Ambiga Somasundaram2Sivasankari Sekar3Sivagurunathan Paramasivam4Pasiyappazham Ramasamy5Department of Biochemistry, Idhaya College for Women, Sarugani, Tamil Nadu 630411, IndiaDepartment of Biochemistry, Sengamala Thayaar Educational Trust Women's college (Autonomous), Mannargudi, Tamil Nadu 614016, IndiaDepartment of Biochemistry, School of Arts and Science, Ponnaiyah Ramajayam Institute of Science and Technology (PRIST), Thanjavur, Tamil Nadu 613403, IndiaMicrobial and Bioprospecting Bioenergy Laboratory, Department of Green Energy Technology, Madanjeet School of Green Energy Technologies, Pondicherry University, Puducherry 605 014, IndiaDepartment of Microbiology, Faculty of Science, Annamalai University, Chidambaram, 608002 Tamil Nadu, IndiaDepartment of Prosthodontics and Implantology, Saveetha Dental College & Hospitals, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, 600077 Tamil Nadu, India; Marine Biopolymer Research Laboratory, Centre for Marine and Aquatic Research (CMAR), Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Chennai, Tamil Nadu, 602105 India; Corresponding author at: Marine Biopolymer Research Laboratory, Centre for Marine and Aquatic Research (CMAR), Saveetha Institue of Mdeical and Technical Sciences (SIMATS), Saveetha University, Chennai, 600077 Tamil Nadu, India.Introduction: The goal of this study was to find out if kaempferol, a compound isolated from the Wattakaka volubilis plant, could protect the liver of rats that were given aluminum sulfate. Methods: Kaempferol, a bioflavonoid, was isolated from the leaves of W. volubilis methanolic extract (MEWV) and characterized by using the High-performance thin-layer chromatography (HPTLC)technique. The liver protective activities of MEWV against aluminum poisoning were investigated in male albino rats, as their protective efficacy was assessed in addition to that of the silymarin standard. The oral administration of silymarin (25 mg/kg), kaempferol (10 mg/kg), and MEWV (200 mg/kg) was administered continuously for 14 days. The body and liver weights as well as serum enzymes like alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were calculated. Results: Advanced techniques successfully isolated and confirmed the bioactive constituent kaempferol from MEWV. When kaempferol and MEWV were given to rats that had been poisoned with aluminum, their body mass index went up significantly (p < 0.001) and the relative weight of their liver and serum marker enzymes went down significantly (p < 0.001) compared to animals that had only been poisoned and analyzed the comparative action of the extract and isolated compound using silymarin, the standard drug. Kaempferol showed better protection against aluminum-induced hepatotoxicity compared to MEWV treatment. Conclusion: In conclusion, the results showed that kaempferol and MEWV reduced the damage done to the liver by aluminum sulfate by protecting cells, the liver, and protecting antioxidants.http://www.sciencedirect.com/science/article/pii/S266703132500082XW. volubilisKaempferolHPTLCAluminium sulphateLiverMarker enzymes |
| spellingShingle | Usharani Saminathan Anuradha Ramamoorthy Ambiga Somasundaram Sivasankari Sekar Sivagurunathan Paramasivam Pasiyappazham Ramasamy Extraction of kaempferol from Wattakaka volubilis and evaluation of its protective effects against aluminium-induced liver toxicity Phytomedicine Plus W. volubilis Kaempferol HPTLC Aluminium sulphate Liver Marker enzymes |
| title | Extraction of kaempferol from Wattakaka volubilis and evaluation of its protective effects against aluminium-induced liver toxicity |
| title_full | Extraction of kaempferol from Wattakaka volubilis and evaluation of its protective effects against aluminium-induced liver toxicity |
| title_fullStr | Extraction of kaempferol from Wattakaka volubilis and evaluation of its protective effects against aluminium-induced liver toxicity |
| title_full_unstemmed | Extraction of kaempferol from Wattakaka volubilis and evaluation of its protective effects against aluminium-induced liver toxicity |
| title_short | Extraction of kaempferol from Wattakaka volubilis and evaluation of its protective effects against aluminium-induced liver toxicity |
| title_sort | extraction of kaempferol from wattakaka volubilis and evaluation of its protective effects against aluminium induced liver toxicity |
| topic | W. volubilis Kaempferol HPTLC Aluminium sulphate Liver Marker enzymes |
| url | http://www.sciencedirect.com/science/article/pii/S266703132500082X |
| work_keys_str_mv | AT usharanisaminathan extractionofkaempferolfromwattakakavolubilisandevaluationofitsprotectiveeffectsagainstaluminiuminducedlivertoxicity AT anuradharamamoorthy extractionofkaempferolfromwattakakavolubilisandevaluationofitsprotectiveeffectsagainstaluminiuminducedlivertoxicity AT ambigasomasundaram extractionofkaempferolfromwattakakavolubilisandevaluationofitsprotectiveeffectsagainstaluminiuminducedlivertoxicity AT sivasankarisekar extractionofkaempferolfromwattakakavolubilisandevaluationofitsprotectiveeffectsagainstaluminiuminducedlivertoxicity AT sivagurunathanparamasivam extractionofkaempferolfromwattakakavolubilisandevaluationofitsprotectiveeffectsagainstaluminiuminducedlivertoxicity AT pasiyappazhamramasamy extractionofkaempferolfromwattakakavolubilisandevaluationofitsprotectiveeffectsagainstaluminiuminducedlivertoxicity |