Long-term prognosis and DNA damage status after oral mucosal epithelial cell sheet transplantation following esophageal endoscopic submucosal dissection for squamous cell carcinoma: A case series
Autologous oral mucosal epithelial cell sheet (AOMECS) transplantation has recently been applied in human patients to prevent postprocedural stenosis following endoscopic submucosal dissection (ESD) for esophageal squamous cell carcinoma. However, the long-term safety of AOMECS transplantation remai...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-06-01
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| Series: | Regenerative Therapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320424001433 |
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| author | Yasuhiro Maruya Yuko Akazawa Kiyuu Norimatsu Yerkezhan Sailaubekova Nazigul Zhumagazhiyeva Shinichiro Kobayashi Miki Higashi Keiichi Hashiguchi Naoyuki Yamaguchi Masahiro Nakashima Kazuhiko Nakao Kengo Kanetaka Susumu Eguchi |
| author_facet | Yasuhiro Maruya Yuko Akazawa Kiyuu Norimatsu Yerkezhan Sailaubekova Nazigul Zhumagazhiyeva Shinichiro Kobayashi Miki Higashi Keiichi Hashiguchi Naoyuki Yamaguchi Masahiro Nakashima Kazuhiko Nakao Kengo Kanetaka Susumu Eguchi |
| author_sort | Yasuhiro Maruya |
| collection | DOAJ |
| description | Autologous oral mucosal epithelial cell sheet (AOMECS) transplantation has recently been applied in human patients to prevent postprocedural stenosis following endoscopic submucosal dissection (ESD) for esophageal squamous cell carcinoma. However, the long-term safety of AOMECS transplantation remains unclear. We evaluated the long-term outcomes of 10 patients who participated in a clinical trial of AOMECS transplantation after esophageal ESD. Additionally, we assessed the local DNA damage response in the esophageal epithelium using p53 binding protein 1 (53BP1) immunofluorescence in post-AOMECS biopsy specimens. The median follow-up period was 118.5 months (range: 46–130 months). Two patients developed primary esophageal cancer near the AOMECS site and successfully underwent additional ESD. One patient developed lymph node metastasis and underwent chemotherapy. None of the patients died from the original disease, although one patient died from unrelated causes. The rate of abnormal 53BP1 nuclear foci, indicative of increased genome instability, increased with the progression of neoplasia in patients post AOMECS. Our case series suggests that AOMECS transplantation provides an acceptable long-term prognosis and 53BP1 foci may serve as a useful marker for assessing DNA instability in the post-AOMECS esophageal epithelium. |
| format | Article |
| id | doaj-art-6bb49679925345468eb87f56c2b02ab2 |
| institution | OA Journals |
| issn | 2352-3204 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj-art-6bb49679925345468eb87f56c2b02ab22025-08-20T02:07:31ZengElsevierRegenerative Therapy2352-32042024-06-012655756310.1016/j.reth.2024.08.007Long-term prognosis and DNA damage status after oral mucosal epithelial cell sheet transplantation following esophageal endoscopic submucosal dissection for squamous cell carcinoma: A case seriesYasuhiro Maruya0Yuko Akazawa1Kiyuu Norimatsu2Yerkezhan Sailaubekova3Nazigul Zhumagazhiyeva4Shinichiro Kobayashi5Miki Higashi6Keiichi Hashiguchi7Naoyuki Yamaguchi8Masahiro Nakashima9Kazuhiko Nakao10Kengo Kanetaka11Susumu Eguchi12Tissue Engineering and Regenerative Therapeutics in Gastrointestinal Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan; Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan; Corresponding author. Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki 852-8501, Japan.Department of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki, JapanDepartment of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanTissue Engineering and Regenerative Therapeutics in Gastrointestinal Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Tumor and Diagnostic Pathology, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki, JapanDepartment of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanTissue Engineering and Regenerative Therapeutics in Gastrointestinal Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan; Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanDepartment of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, JapanAutologous oral mucosal epithelial cell sheet (AOMECS) transplantation has recently been applied in human patients to prevent postprocedural stenosis following endoscopic submucosal dissection (ESD) for esophageal squamous cell carcinoma. However, the long-term safety of AOMECS transplantation remains unclear. We evaluated the long-term outcomes of 10 patients who participated in a clinical trial of AOMECS transplantation after esophageal ESD. Additionally, we assessed the local DNA damage response in the esophageal epithelium using p53 binding protein 1 (53BP1) immunofluorescence in post-AOMECS biopsy specimens. The median follow-up period was 118.5 months (range: 46–130 months). Two patients developed primary esophageal cancer near the AOMECS site and successfully underwent additional ESD. One patient developed lymph node metastasis and underwent chemotherapy. None of the patients died from the original disease, although one patient died from unrelated causes. The rate of abnormal 53BP1 nuclear foci, indicative of increased genome instability, increased with the progression of neoplasia in patients post AOMECS. Our case series suggests that AOMECS transplantation provides an acceptable long-term prognosis and 53BP1 foci may serve as a useful marker for assessing DNA instability in the post-AOMECS esophageal epithelium.http://www.sciencedirect.com/science/article/pii/S2352320424001433Cell sheetEsophageal squamous cell carcinoma53BP1 |
| spellingShingle | Yasuhiro Maruya Yuko Akazawa Kiyuu Norimatsu Yerkezhan Sailaubekova Nazigul Zhumagazhiyeva Shinichiro Kobayashi Miki Higashi Keiichi Hashiguchi Naoyuki Yamaguchi Masahiro Nakashima Kazuhiko Nakao Kengo Kanetaka Susumu Eguchi Long-term prognosis and DNA damage status after oral mucosal epithelial cell sheet transplantation following esophageal endoscopic submucosal dissection for squamous cell carcinoma: A case series Regenerative Therapy Cell sheet Esophageal squamous cell carcinoma 53BP1 |
| title | Long-term prognosis and DNA damage status after oral mucosal epithelial cell sheet transplantation following esophageal endoscopic submucosal dissection for squamous cell carcinoma: A case series |
| title_full | Long-term prognosis and DNA damage status after oral mucosal epithelial cell sheet transplantation following esophageal endoscopic submucosal dissection for squamous cell carcinoma: A case series |
| title_fullStr | Long-term prognosis and DNA damage status after oral mucosal epithelial cell sheet transplantation following esophageal endoscopic submucosal dissection for squamous cell carcinoma: A case series |
| title_full_unstemmed | Long-term prognosis and DNA damage status after oral mucosal epithelial cell sheet transplantation following esophageal endoscopic submucosal dissection for squamous cell carcinoma: A case series |
| title_short | Long-term prognosis and DNA damage status after oral mucosal epithelial cell sheet transplantation following esophageal endoscopic submucosal dissection for squamous cell carcinoma: A case series |
| title_sort | long term prognosis and dna damage status after oral mucosal epithelial cell sheet transplantation following esophageal endoscopic submucosal dissection for squamous cell carcinoma a case series |
| topic | Cell sheet Esophageal squamous cell carcinoma 53BP1 |
| url | http://www.sciencedirect.com/science/article/pii/S2352320424001433 |
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