Proteomics of colorectal tumors identifies the role of CAVIN1 in tumor relapse
Abstract Colorectal cancer molecular signatures derived from omics data can be employed to stratify CRC patients and aid decisions about therapies or evaluate prognostic outcome. However, molecular biomarkers for identification of patients at increased risk of disease relapse are currently lacking....
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| Format: | Article |
| Language: | English |
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Springer Nature
2025-04-01
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| Series: | Molecular Systems Biology |
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| Online Access: | https://doi.org/10.1038/s44320-025-00102-8 |
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| _version_ | 1849238145613168640 |
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| author | Ana Martinez-Val Leander Van der Hoeven Dorte B Bekker-Jensen Margarita Melnikova Jørgensen Jesper Nors Giulia Franciosa Claus L Andersen Jesper B Bramsen Jesper V Olsen |
| author_facet | Ana Martinez-Val Leander Van der Hoeven Dorte B Bekker-Jensen Margarita Melnikova Jørgensen Jesper Nors Giulia Franciosa Claus L Andersen Jesper B Bramsen Jesper V Olsen |
| author_sort | Ana Martinez-Val |
| collection | DOAJ |
| description | Abstract Colorectal cancer molecular signatures derived from omics data can be employed to stratify CRC patients and aid decisions about therapies or evaluate prognostic outcome. However, molecular biomarkers for identification of patients at increased risk of disease relapse are currently lacking. Here, we present a comprehensive multi-omics analysis of a Danish colorectal cancer tumor cohort composed of 412 biopsies from tumors of 371 patients diagnosed at TNM stage II or III. From mass spectrometry-based patient proteome profiles, we classified the tumors into four molecular subtypes, including a mesenchymal-like subtype. As the mesenchymal-rich tumors are known to represent the most invasive and metastatic phenotype, we focused on the protein signature defining this subtype to evaluate their potential as relapse risk markers. Among signature-specific proteins, we followed-up Caveolae-Associated Protein-1 (CAVIN1) and demonstrated its role in tumor progression in a 3D in vitro model of colorectal cancer. Compared to previous omics analyses of CRC, our multi-omics classification provided deeper insights into EMT in cancer cells with stronger correlations with risk of relapse. |
| format | Article |
| id | doaj-art-6bb2adc6f342410cb0adc940014499c7 |
| institution | Kabale University |
| issn | 1744-4292 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-6bb2adc6f342410cb0adc940014499c72025-08-20T04:01:43ZengSpringer NatureMolecular Systems Biology1744-42922025-04-0121777680610.1038/s44320-025-00102-8Proteomics of colorectal tumors identifies the role of CAVIN1 in tumor relapseAna Martinez-Val0Leander Van der Hoeven1Dorte B Bekker-Jensen2Margarita Melnikova Jørgensen3Jesper Nors4Giulia Franciosa5Claus L Andersen6Jesper B Bramsen7Jesper V Olsen8Novo Nordisk Foundation Center for Protein Research, Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of CopenhagenNovo Nordisk Foundation Center for Protein Research, Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of CopenhagenNovo Nordisk Foundation Center for Protein Research, Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of CopenhagenInstitute of Pathology, Randers Regional HospitalDepartment of Clinical Medicine, Aarhus University HospitalNovo Nordisk Foundation Center for Protein Research, Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of CopenhagenDepartment of Clinical Medicine, Aarhus University HospitalDepartment of Clinical Medicine, Aarhus University HospitalNovo Nordisk Foundation Center for Protein Research, Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of CopenhagenAbstract Colorectal cancer molecular signatures derived from omics data can be employed to stratify CRC patients and aid decisions about therapies or evaluate prognostic outcome. However, molecular biomarkers for identification of patients at increased risk of disease relapse are currently lacking. Here, we present a comprehensive multi-omics analysis of a Danish colorectal cancer tumor cohort composed of 412 biopsies from tumors of 371 patients diagnosed at TNM stage II or III. From mass spectrometry-based patient proteome profiles, we classified the tumors into four molecular subtypes, including a mesenchymal-like subtype. As the mesenchymal-rich tumors are known to represent the most invasive and metastatic phenotype, we focused on the protein signature defining this subtype to evaluate their potential as relapse risk markers. Among signature-specific proteins, we followed-up Caveolae-Associated Protein-1 (CAVIN1) and demonstrated its role in tumor progression in a 3D in vitro model of colorectal cancer. Compared to previous omics analyses of CRC, our multi-omics classification provided deeper insights into EMT in cancer cells with stronger correlations with risk of relapse.https://doi.org/10.1038/s44320-025-00102-8Colorectal CancerProteomicsTumor RelapseCAVIN1 |
| spellingShingle | Ana Martinez-Val Leander Van der Hoeven Dorte B Bekker-Jensen Margarita Melnikova Jørgensen Jesper Nors Giulia Franciosa Claus L Andersen Jesper B Bramsen Jesper V Olsen Proteomics of colorectal tumors identifies the role of CAVIN1 in tumor relapse Molecular Systems Biology Colorectal Cancer Proteomics Tumor Relapse CAVIN1 |
| title | Proteomics of colorectal tumors identifies the role of CAVIN1 in tumor relapse |
| title_full | Proteomics of colorectal tumors identifies the role of CAVIN1 in tumor relapse |
| title_fullStr | Proteomics of colorectal tumors identifies the role of CAVIN1 in tumor relapse |
| title_full_unstemmed | Proteomics of colorectal tumors identifies the role of CAVIN1 in tumor relapse |
| title_short | Proteomics of colorectal tumors identifies the role of CAVIN1 in tumor relapse |
| title_sort | proteomics of colorectal tumors identifies the role of cavin1 in tumor relapse |
| topic | Colorectal Cancer Proteomics Tumor Relapse CAVIN1 |
| url | https://doi.org/10.1038/s44320-025-00102-8 |
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