Longitudinal calcium imaging of BNST neurons in mice during optogenetic manipulation of paraventricular thalamus axon terminals
Summary: Here, we present a protocol to study neural circuits between the paraventricular thalamus (PVA) and the bed nucleus of the stria terminalis (BNST) in mice by combining calcium imaging with optogenetic stimulation of axon terminals. We describe steps for delivering GCaMP6f and ChrimsonR viru...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725002060 |
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