ANTIOXIDANT ACTIVITY DUAL METHODS OF DIFFERENT POLARITY EXTRACTS OF STEM OF SOLANUM NIGRUM L. GROWING IN ALGERIA
The purpose of this study is to evaluate the antioxidants properties and phenolic and flavonoid contents of Solanum nigrum L. growing in El-Oued region (south east of Algeria). The different polarity extracts chloroform (CHCl3), ethyl acetate (AcOEt) and n-butanol (n-BuOH) have been prepared from th...
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Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
2021-07-01
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| Series: | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
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| Online Access: | https://pubs.ub.ro/?pg=revues&rev=cscc6&num=202102&vol=2&aid=5276 |
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| author | IMANE GUEDIRI CHERIFA BOUBEKRI OUANISSA SMARA |
| author_facet | IMANE GUEDIRI CHERIFA BOUBEKRI OUANISSA SMARA |
| author_sort | IMANE GUEDIRI |
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| description | The purpose of this study is to evaluate the antioxidants properties and phenolic and flavonoid contents of Solanum nigrum L. growing in El-Oued region (south east of Algeria). The different polarity extracts chloroform (CHCl3), ethyl acetate (AcOEt) and n-butanol (n-BuOH) have been prepared from the aqueous ethanol extract of the stem. The antioxidant activity of extracts was determined by DPPH (2, 2-diphenyl-1-picrylhydrazyl), FRAP (Ferric ion reducing antioxidant power) assays. The results showed that CHCl3 extract has the highest level of polyphenol (66.427 ± 0.001 mg gallic acid / g) while, the AcOEt extract has the highest level of flavonoid content (26.450 ± 0.079 mg rutin equivalents / g). In addition, the highest IC50 of DPPH scavenging activity has exhibited the most potent antioxidant capacity assay of CHCl3 (0.231 mg∙mL-1) compared to the n-BuOH and AcOEt extracts. Furthermore, the highest value of FRAP for AcOEt extract was 458.291 ± 0.005. In this study, it has been noted that the potency of the antioxidant activity of DPPH may be identified through different levels of polyphenol. However, the value of FRAP has a relation to the value of flavonoid. |
| format | Article |
| id | doaj-art-6ba4c98e9c16440d91bc8f86e6b0a4e9 |
| institution | OA Journals |
| issn | 1582-540X 1582-540X |
| language | English |
| publishDate | 2021-07-01 |
| publisher | Alma Mater Publishing House "Vasile Alecsandri" University of Bacau |
| record_format | Article |
| series | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
| spelling | doaj-art-6ba4c98e9c16440d91bc8f86e6b0a4e92025-08-20T02:15:37ZengAlma Mater Publishing House "Vasile Alecsandri" University of BacauScientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry1582-540X1582-540X2021-07-01222227234ANTIOXIDANT ACTIVITY DUAL METHODS OF DIFFERENT POLARITY EXTRACTS OF STEM OF SOLANUM NIGRUM L. GROWING IN ALGERIAIMANE GUEDIRI 0 CHERIFA BOUBEKRI 1OUANISSA SMARA 21. Ouargla University, Faculty of Material Science, Chemistry Department, VPRS Laboratory, Ghardaia Road BP 511, 30000, Ouargla, Algeria 2. El Oued University, Faculty of Technology, Process Engineering Department, VTRS Laboratory, PO Box 789, 39000, El Oued, Algeria 2. El Oued University, Faculty of Technology, Process Engineering Department, VTRS Laboratory, PO Box 789, 39000, El Oued, Algeria1. Ouargla University, Faculty of Material Science, Chemistry Department, VPRS Laboratory, Ghardaia Road BP 511, 30000, Ouargla, Algeria The purpose of this study is to evaluate the antioxidants properties and phenolic and flavonoid contents of Solanum nigrum L. growing in El-Oued region (south east of Algeria). The different polarity extracts chloroform (CHCl3), ethyl acetate (AcOEt) and n-butanol (n-BuOH) have been prepared from the aqueous ethanol extract of the stem. The antioxidant activity of extracts was determined by DPPH (2, 2-diphenyl-1-picrylhydrazyl), FRAP (Ferric ion reducing antioxidant power) assays. The results showed that CHCl3 extract has the highest level of polyphenol (66.427 ± 0.001 mg gallic acid / g) while, the AcOEt extract has the highest level of flavonoid content (26.450 ± 0.079 mg rutin equivalents / g). In addition, the highest IC50 of DPPH scavenging activity has exhibited the most potent antioxidant capacity assay of CHCl3 (0.231 mg∙mL-1) compared to the n-BuOH and AcOEt extracts. Furthermore, the highest value of FRAP for AcOEt extract was 458.291 ± 0.005. In this study, it has been noted that the potency of the antioxidant activity of DPPH may be identified through different levels of polyphenol. However, the value of FRAP has a relation to the value of flavonoid.https://pubs.ub.ro/?pg=revues&rev=cscc6&num=202102&vol=2&aid=5276antioxidants propertiesdpphel-oued regionflavonoidfrappolyphenolsolanum nigrum l.stem |
| spellingShingle | IMANE GUEDIRI CHERIFA BOUBEKRI OUANISSA SMARA ANTIOXIDANT ACTIVITY DUAL METHODS OF DIFFERENT POLARITY EXTRACTS OF STEM OF SOLANUM NIGRUM L. GROWING IN ALGERIA Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry antioxidants properties dpph el-oued region flavonoid frap polyphenol solanum nigrum l. stem |
| title | ANTIOXIDANT ACTIVITY DUAL METHODS OF DIFFERENT POLARITY EXTRACTS OF STEM OF SOLANUM NIGRUM L. GROWING IN ALGERIA |
| title_full | ANTIOXIDANT ACTIVITY DUAL METHODS OF DIFFERENT POLARITY EXTRACTS OF STEM OF SOLANUM NIGRUM L. GROWING IN ALGERIA |
| title_fullStr | ANTIOXIDANT ACTIVITY DUAL METHODS OF DIFFERENT POLARITY EXTRACTS OF STEM OF SOLANUM NIGRUM L. GROWING IN ALGERIA |
| title_full_unstemmed | ANTIOXIDANT ACTIVITY DUAL METHODS OF DIFFERENT POLARITY EXTRACTS OF STEM OF SOLANUM NIGRUM L. GROWING IN ALGERIA |
| title_short | ANTIOXIDANT ACTIVITY DUAL METHODS OF DIFFERENT POLARITY EXTRACTS OF STEM OF SOLANUM NIGRUM L. GROWING IN ALGERIA |
| title_sort | antioxidant activity dual methods of different polarity extracts of stem of solanum nigrum l growing in algeria |
| topic | antioxidants properties dpph el-oued region flavonoid frap polyphenol solanum nigrum l. stem |
| url | https://pubs.ub.ro/?pg=revues&rev=cscc6&num=202102&vol=2&aid=5276 |
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