Tracking Dengue Virus Intra-host Genetic Diversity during Human-to-Mosquito Transmission.
Dengue virus (DENV) infection of an individual human or mosquito host produces a dynamic population of closely-related sequences. This intra-host genetic diversity is thought to offer an advantage for arboviruses to adapt as they cycle between two very different host species, but it remains poorly c...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0004052 |
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| author | Shuzhen Sim Pauline P K Aw Andreas Wilm Garrett Teoh Kien Duong Thi Hue Nguyet Minh Nguyen Niranjan Nagarajan Cameron P Simmons Martin L Hibberd |
| author_facet | Shuzhen Sim Pauline P K Aw Andreas Wilm Garrett Teoh Kien Duong Thi Hue Nguyet Minh Nguyen Niranjan Nagarajan Cameron P Simmons Martin L Hibberd |
| author_sort | Shuzhen Sim |
| collection | DOAJ |
| description | Dengue virus (DENV) infection of an individual human or mosquito host produces a dynamic population of closely-related sequences. This intra-host genetic diversity is thought to offer an advantage for arboviruses to adapt as they cycle between two very different host species, but it remains poorly characterized. To track changes in viral intra-host genetic diversity during horizontal transmission, we infected Aedes aegypti mosquitoes by allowing them to feed on DENV2-infected patients. We then performed whole-genome deep-sequencing of human- and matched mosquito-derived DENV samples on the Illumina platform and used a sensitive variant-caller to detect single nucleotide variants (SNVs) within each sample. >90% of SNVs were lost upon transition from human to mosquito, as well as from mosquito abdomen to salivary glands. Levels of viral diversity were maintained, however, by the regeneration of new SNVs at each stage of transmission. We further show that SNVs maintained across transmission stages were transmitted as a unit of two at maximum, suggesting the presence of numerous variant genomes carrying only one or two SNVs each. We also present evidence for differences in selection pressures between human and mosquito hosts, particularly on the structural and NS1 genes. This analysis provides insights into how population drops during transmission shape RNA virus genetic diversity, has direct implications for virus evolution, and illustrates the value of high-coverage, whole-genome next-generation sequencing for understanding viral intra-host genetic diversity. |
| format | Article |
| id | doaj-art-6b99b27797eb47a79b60d25f26a0f3d7 |
| institution | OA Journals |
| issn | 1935-2727 1935-2735 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj-art-6b99b27797eb47a79b60d25f26a0f3d72025-08-20T02:22:37ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352015-01-0199e000405210.1371/journal.pntd.0004052Tracking Dengue Virus Intra-host Genetic Diversity during Human-to-Mosquito Transmission.Shuzhen SimPauline P K AwAndreas WilmGarrett TeohKien Duong Thi HueNguyet Minh NguyenNiranjan NagarajanCameron P SimmonsMartin L HibberdDengue virus (DENV) infection of an individual human or mosquito host produces a dynamic population of closely-related sequences. This intra-host genetic diversity is thought to offer an advantage for arboviruses to adapt as they cycle between two very different host species, but it remains poorly characterized. To track changes in viral intra-host genetic diversity during horizontal transmission, we infected Aedes aegypti mosquitoes by allowing them to feed on DENV2-infected patients. We then performed whole-genome deep-sequencing of human- and matched mosquito-derived DENV samples on the Illumina platform and used a sensitive variant-caller to detect single nucleotide variants (SNVs) within each sample. >90% of SNVs were lost upon transition from human to mosquito, as well as from mosquito abdomen to salivary glands. Levels of viral diversity were maintained, however, by the regeneration of new SNVs at each stage of transmission. We further show that SNVs maintained across transmission stages were transmitted as a unit of two at maximum, suggesting the presence of numerous variant genomes carrying only one or two SNVs each. We also present evidence for differences in selection pressures between human and mosquito hosts, particularly on the structural and NS1 genes. This analysis provides insights into how population drops during transmission shape RNA virus genetic diversity, has direct implications for virus evolution, and illustrates the value of high-coverage, whole-genome next-generation sequencing for understanding viral intra-host genetic diversity.https://doi.org/10.1371/journal.pntd.0004052 |
| spellingShingle | Shuzhen Sim Pauline P K Aw Andreas Wilm Garrett Teoh Kien Duong Thi Hue Nguyet Minh Nguyen Niranjan Nagarajan Cameron P Simmons Martin L Hibberd Tracking Dengue Virus Intra-host Genetic Diversity during Human-to-Mosquito Transmission. PLoS Neglected Tropical Diseases |
| title | Tracking Dengue Virus Intra-host Genetic Diversity during Human-to-Mosquito Transmission. |
| title_full | Tracking Dengue Virus Intra-host Genetic Diversity during Human-to-Mosquito Transmission. |
| title_fullStr | Tracking Dengue Virus Intra-host Genetic Diversity during Human-to-Mosquito Transmission. |
| title_full_unstemmed | Tracking Dengue Virus Intra-host Genetic Diversity during Human-to-Mosquito Transmission. |
| title_short | Tracking Dengue Virus Intra-host Genetic Diversity during Human-to-Mosquito Transmission. |
| title_sort | tracking dengue virus intra host genetic diversity during human to mosquito transmission |
| url | https://doi.org/10.1371/journal.pntd.0004052 |
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