Transcriptional responses of Metarhizium pingshaense blastospores after UV-B irradiation

Transcriptional responses of Metarhizium pingshaense blastospores after UV-B irradiation

Metarhizium is widely known for its role as an arthropod biocontrol agent and plant bioinoculant. By using mass-production industrial methods, it is possible to produce large amounts of fungal single-celled propagules (including blastospores) to be applied in the field. However, in the environment,...

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Main Authors: Amanda Rocha da Costa Corval, Lucas Amoroso Lopes de Carvalho, Emily Mesquita, Jéssica Fiorotti, Thaís Almeida Corrêa, Victória Silvestre Bório, Adriani da Silva Carneiro, Daniel Guariz Pinheiro, Irene da Silva Coelho, Huarrisson Azevedo Santos, Everton Kort Kamp Fernandes, Isabele da Costa Angelo, Vânia R. E. P. Bittencourt, Patrícia Silva Golo
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-12-01
Series:Frontiers in Microbiology
Subjects:
entomopathogenic fungi
insect control
RNA-seq
tolerance to UV-B
bioinputs
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2024.1507931/full
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Summary:Metarhizium is widely known for its role as an arthropod biocontrol agent and plant bioinoculant. By using mass-production industrial methods, it is possible to produce large amounts of fungal single-celled propagules (including blastospores) to be applied in the field. However, in the environment, the solar ultraviolet components (particularly UV-B) can harm the fungus, negatively impacting its pathogenicity toward the arthropod pest. The present study is the first to use comparative genome-wide transcriptome analyses to unveil changes in gene expression between Metarhizium pingshaense blastospores exposed or not to UV-B. Relative blastospores culturability was calculated 72 h after UV-B exposure and exhibited 100% culturability. In total, 6.57% (n = 728) out of 11,076 predicted genes in M. pingshaense were differentially expressed after UV-B exposure: 320 genes (44%; 320/728) were upregulated and 408 (56%; 408/720) were downregulated in the UV-B exposed blastospores. Results unveiled differentially expressed gene sets related to fungal virulence, production of secondary metabolites, and DNA repair associated with UV damage; genes related to virulence factors were downregulated, and genes associated with nucleotide excision repair were upregulated. These findings illustrate critical aspects of Metarhizium blastospores strategies to overcome UV-B damage and survive solar radiation exposures in insulated fields.
ISSN:1664-302X

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