Planar arrangement of eukaryotic cells in merged hydrogels combines the advantages of 3-D and 2-D cultures
We report an unordered 2-D array of eukaryotic cells completely embedded in a 3-D matrix. Every cell is located at the same distance from the gel surface, which ensures uniformity of growth conditions and ease of observation characteristic of a 2-D culture. Each cell is firmly immobilized, and each...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2012-05-01
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| Series: | BioTechniques |
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| Online Access: | https://www.future-science.com/doi/10.2144/000113861 |
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| author | Alexander A. Gordeev Helena V. Chetverina Alexander B. Chetverin |
| author_facet | Alexander A. Gordeev Helena V. Chetverina Alexander B. Chetverin |
| author_sort | Alexander A. Gordeev |
| collection | DOAJ |
| description | We report an unordered 2-D array of eukaryotic cells completely embedded in a 3-D matrix. Every cell is located at the same distance from the gel surface, which ensures uniformity of growth conditions and ease of observation characteristic of a 2-D culture. Each cell is firmly immobilized, and each has a unique address in the array. The cells can be rapidly screened, individually monitored during extended time periods, and cultured with the formation of spheroid microcolonies characteristic of a 3-D culture. Individual microcolonies can be extracted from the gel and further propagated, thus enabling isolation of pure cell clones from rather dense cell populations and rapid drug-free generation of stable cell lines. |
| format | Article |
| id | doaj-art-6b7e310759ee46edb9f4f77d6caaa23d |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2012-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-6b7e310759ee46edb9f4f77d6caaa23d2025-08-20T02:25:37ZengTaylor & Francis GroupBioTechniques0736-62051940-98182012-05-0152532533110.2144/000113861Planar arrangement of eukaryotic cells in merged hydrogels combines the advantages of 3-D and 2-D culturesAlexander A. Gordeev0Helena V. Chetverina1Alexander B. Chetverin21Institute of Protein Research of the Russian Academy of Sciences, Pushchino, Moscow Region, Russia1Institute of Protein Research of the Russian Academy of Sciences, Pushchino, Moscow Region, Russia1Institute of Protein Research of the Russian Academy of Sciences, Pushchino, Moscow Region, RussiaWe report an unordered 2-D array of eukaryotic cells completely embedded in a 3-D matrix. Every cell is located at the same distance from the gel surface, which ensures uniformity of growth conditions and ease of observation characteristic of a 2-D culture. Each cell is firmly immobilized, and each has a unique address in the array. The cells can be rapidly screened, individually monitored during extended time periods, and cultured with the formation of spheroid microcolonies characteristic of a 3-D culture. Individual microcolonies can be extracted from the gel and further propagated, thus enabling isolation of pure cell clones from rather dense cell populations and rapid drug-free generation of stable cell lines.https://www.future-science.com/doi/10.2144/000113861suspension cellsadherent cellspopulation studiescell immobilizationhigh-throughput screeningcell lines |
| spellingShingle | Alexander A. Gordeev Helena V. Chetverina Alexander B. Chetverin Planar arrangement of eukaryotic cells in merged hydrogels combines the advantages of 3-D and 2-D cultures BioTechniques suspension cells adherent cells population studies cell immobilization high-throughput screening cell lines |
| title | Planar arrangement of eukaryotic cells in merged hydrogels combines the advantages of 3-D and 2-D cultures |
| title_full | Planar arrangement of eukaryotic cells in merged hydrogels combines the advantages of 3-D and 2-D cultures |
| title_fullStr | Planar arrangement of eukaryotic cells in merged hydrogels combines the advantages of 3-D and 2-D cultures |
| title_full_unstemmed | Planar arrangement of eukaryotic cells in merged hydrogels combines the advantages of 3-D and 2-D cultures |
| title_short | Planar arrangement of eukaryotic cells in merged hydrogels combines the advantages of 3-D and 2-D cultures |
| title_sort | planar arrangement of eukaryotic cells in merged hydrogels combines the advantages of 3 d and 2 d cultures |
| topic | suspension cells adherent cells population studies cell immobilization high-throughput screening cell lines |
| url | https://www.future-science.com/doi/10.2144/000113861 |
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