Mesenchymal Stem Cells Immunosuppressed IL-22 in Patients with Immune Thrombocytopenia via Soluble Cellular Factors
Mesenchymal stem cells are immunoregulation cells. IL-22 plays an important role in the pathogenesis of immune thrombocytopenia. However, the effects of mesenchymal stem cells on IL-22 production in patients with immune thrombocytopenia remain unclear. Flow cytometry analyzed immunophenotypes of mes...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/316351 |
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| author | Mei Wu Hongfeng Ge Shue Li Hailiang Chu Shili Yang Xiaoxing Sun Zhenxia Zhou Xiongpeng Zhu |
| author_facet | Mei Wu Hongfeng Ge Shue Li Hailiang Chu Shili Yang Xiaoxing Sun Zhenxia Zhou Xiongpeng Zhu |
| author_sort | Mei Wu |
| collection | DOAJ |
| description | Mesenchymal stem cells are immunoregulation cells. IL-22 plays an important role in the pathogenesis of immune thrombocytopenia. However, the effects of mesenchymal stem cells on IL-22 production in patients with immune thrombocytopenia remain unclear. Flow cytometry analyzed immunophenotypes of mesenchymal stem cells; differentiation of mesenchymal stem cells was observed by oil red O and Alizarin red S staining; cell proliferation suppression was measured with MTS; IL-22 levels of cell-free supernatants were determined by ELISA. Mesenchymal stem cells inhibited the proliferation of activated CD4+T cells; moreover, mesenchymal stem cells immunosuppressed IL-22 by soluble cellular factors but not PGE2. These results suggest that mesenchymal stem cells may be a therapeutic strategy for patients with immune thrombocytopenia. |
| format | Article |
| id | doaj-art-6b7cbe15d016422ab0a8f3adbba033c4 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-6b7cbe15d016422ab0a8f3adbba033c42025-08-20T03:34:00ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/316351316351Mesenchymal Stem Cells Immunosuppressed IL-22 in Patients with Immune Thrombocytopenia via Soluble Cellular FactorsMei Wu0Hongfeng Ge1Shue Li2Hailiang Chu3Shili Yang4Xiaoxing Sun5Zhenxia Zhou6Xiongpeng Zhu7Department of Hematology, The People’s Hospital of Bozhou, Bozhou 236800, ChinaDepartment of Hematology, The People’s Hospital of Bozhou, Bozhou 236800, ChinaDepartment of Hematology, The People’s Hospital of Bozhou, Bozhou 236800, ChinaDepartment of Hematology, The People’s Hospital of Bozhou, Bozhou 236800, ChinaDepartment of Hematology, The People’s Hospital of Bozhou, Bozhou 236800, ChinaDepartment of Hematology, The People’s Hospital of Bozhou, Bozhou 236800, ChinaDepartment of Hematology, The People’s Hospital of Bozhou, Bozhou 236800, ChinaDepartment of Hematology, First Hospital of Quanzhou Affiliated to Fujian Medical University, Quanzhou 362000, ChinaMesenchymal stem cells are immunoregulation cells. IL-22 plays an important role in the pathogenesis of immune thrombocytopenia. However, the effects of mesenchymal stem cells on IL-22 production in patients with immune thrombocytopenia remain unclear. Flow cytometry analyzed immunophenotypes of mesenchymal stem cells; differentiation of mesenchymal stem cells was observed by oil red O and Alizarin red S staining; cell proliferation suppression was measured with MTS; IL-22 levels of cell-free supernatants were determined by ELISA. Mesenchymal stem cells inhibited the proliferation of activated CD4+T cells; moreover, mesenchymal stem cells immunosuppressed IL-22 by soluble cellular factors but not PGE2. These results suggest that mesenchymal stem cells may be a therapeutic strategy for patients with immune thrombocytopenia.http://dx.doi.org/10.1155/2015/316351 |
| spellingShingle | Mei Wu Hongfeng Ge Shue Li Hailiang Chu Shili Yang Xiaoxing Sun Zhenxia Zhou Xiongpeng Zhu Mesenchymal Stem Cells Immunosuppressed IL-22 in Patients with Immune Thrombocytopenia via Soluble Cellular Factors Journal of Immunology Research |
| title | Mesenchymal Stem Cells Immunosuppressed IL-22 in Patients with Immune Thrombocytopenia via Soluble Cellular Factors |
| title_full | Mesenchymal Stem Cells Immunosuppressed IL-22 in Patients with Immune Thrombocytopenia via Soluble Cellular Factors |
| title_fullStr | Mesenchymal Stem Cells Immunosuppressed IL-22 in Patients with Immune Thrombocytopenia via Soluble Cellular Factors |
| title_full_unstemmed | Mesenchymal Stem Cells Immunosuppressed IL-22 in Patients with Immune Thrombocytopenia via Soluble Cellular Factors |
| title_short | Mesenchymal Stem Cells Immunosuppressed IL-22 in Patients with Immune Thrombocytopenia via Soluble Cellular Factors |
| title_sort | mesenchymal stem cells immunosuppressed il 22 in patients with immune thrombocytopenia via soluble cellular factors |
| url | http://dx.doi.org/10.1155/2015/316351 |
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