Single-cell protein-DNA interactomics and multiomics tools for deciphering genome regulation

The emergence of single-cell genomic and transcriptomic sequencing accelerates the development of single-cell epigenomic technologies, providing an unprecedented opportunity for decoding cell fate decisions largely encoded in the epigenome. Recent advances in single-cell multimodality epigenomic tec...

Full description

Saved in:
Bibliographic Details
Main Authors: Xiong Haiqing, Wang Runyu, He Aibin
Format: Article
Language:English
Published: Science Press 2023-03-01
Series:National Science Open
Subjects:
Online Access:https://www.sciengine.com/doi/10.1360/nso/20220057
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The emergence of single-cell genomic and transcriptomic sequencing accelerates the development of single-cell epigenomic technologies, providing an unprecedented opportunity for decoding cell fate decisions largely encoded in the epigenome. Recent advances in single-cell multimodality epigenomic technologies facilitate directly interrogating the regulatory relationship between multi-layer molecular information in the same cell. In this review, we discuss recent progress in development of single-cell multimodality epigenomic technologies and applications in elucidating cellular diversifications in development and diseases, with a focus on protein-DNA interactomics and regulatory links between epigenome and transcriptome. Further, we provide perspective on the future direction of single-cell multiomics tool development as well as challenges facing ahead.
ISSN:2097-1168