Hydrophylic interaction chromatography used for separation of hydroxycarbamide and urea
The article investigates the possibility of using the chromatographic column XBridge Amide 150´4.6 mm (3.5 μm) for separation of hydroxycarbamide (hydroxyurea) and its impurity (urea). The mobile phases used were the mixtures of acetonitrile and water. The separation of analytes on an amide column b...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | Russian |
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Federal State Budgetary Institution ‘Scientific Centre for Expert Evaluation of Medicinal Products’ of the Ministry of Health of the Russian Federation (FSBI ‘SCEEMP’)
2018-02-01
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| Series: | Регуляторные исследования и экспертиза лекарственных средств |
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| Online Access: | https://www.vedomostincesmp.ru/jour/article/view/123 |
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| _version_ | 1849393950879645696 |
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| author | A. S. Osipov O. A. Popova S. G. Larionova E. Yu. Timoshina |
| author_facet | A. S. Osipov O. A. Popova S. G. Larionova E. Yu. Timoshina |
| author_sort | A. S. Osipov |
| collection | DOAJ |
| description | The article investigates the possibility of using the chromatographic column XBridge Amide 150´4.6 mm (3.5 μm) for separation of hydroxycarbamide (hydroxyurea) and its impurity (urea). The mobile phases used were the mixtures of acetonitrile and water. The separation of analytes on an amide column by hydrophilic interaction liquid chromatography is feasible if the proportion of acetonitrile in the mobile phase accounts for more than 93 %. When performing chromatographic separation on the amide column XBridge Amide, a change in the elution order of hydroxycarbamide and urea was observed, as compared to the elution on nitrile and diol columns. This type of columns can be used for chromatographic separation of a mixture of hydroxycarbamide and urea in order to perform system suitability testing when analyzing urea by HPLC. |
| format | Article |
| id | doaj-art-6b3b48ddf3ac478fac7dbc21e224587e |
| institution | Kabale University |
| issn | 3034-3062 3034-3453 |
| language | Russian |
| publishDate | 2018-02-01 |
| publisher | Federal State Budgetary Institution ‘Scientific Centre for Expert Evaluation of Medicinal Products’ of the Ministry of Health of the Russian Federation (FSBI ‘SCEEMP’) |
| record_format | Article |
| series | Регуляторные исследования и экспертиза лекарственных средств |
| spelling | doaj-art-6b3b48ddf3ac478fac7dbc21e224587e2025-08-20T03:40:14ZrusFederal State Budgetary Institution ‘Scientific Centre for Expert Evaluation of Medicinal Products’ of the Ministry of Health of the Russian Federation (FSBI ‘SCEEMP’)Регуляторные исследования и экспертиза лекарственных средств3034-30623034-34532018-02-01728184121Hydrophylic interaction chromatography used for separation of hydroxycarbamide and ureaA. S. Osipov0O. A. Popova1S. G. Larionova2E. Yu. Timoshina3Scientific Centre for Expert Evaluation of Medicinal ProductsScientific Centre for Expert Evaluation of Medicinal ProductsScientific Centre for Expert Evaluation of Medicinal ProductsScientific Centre for Expert Evaluation of Medicinal ProductsThe article investigates the possibility of using the chromatographic column XBridge Amide 150´4.6 mm (3.5 μm) for separation of hydroxycarbamide (hydroxyurea) and its impurity (urea). The mobile phases used were the mixtures of acetonitrile and water. The separation of analytes on an amide column by hydrophilic interaction liquid chromatography is feasible if the proportion of acetonitrile in the mobile phase accounts for more than 93 %. When performing chromatographic separation on the amide column XBridge Amide, a change in the elution order of hydroxycarbamide and urea was observed, as compared to the elution on nitrile and diol columns. This type of columns can be used for chromatographic separation of a mixture of hydroxycarbamide and urea in order to perform system suitability testing when analyzing urea by HPLC.https://www.vedomostincesmp.ru/jour/article/view/123высокоэффективная жидкостная хроматографияжидкостная хроматография гидрофильных взаимодействийгидроксикарбамидмочевинафармакопеяhigh performance liquid chromatographyhydrophilic interaction liquid chromatographyhydroxycarbamidehydroxyureapharmacopoeia |
| spellingShingle | A. S. Osipov O. A. Popova S. G. Larionova E. Yu. Timoshina Hydrophylic interaction chromatography used for separation of hydroxycarbamide and urea Регуляторные исследования и экспертиза лекарственных средств высокоэффективная жидкостная хроматография жидкостная хроматография гидрофильных взаимодействий гидроксикарбамид мочевина фармакопея high performance liquid chromatography hydrophilic interaction liquid chromatography hydroxycarbamide hydroxyurea pharmacopoeia |
| title | Hydrophylic interaction chromatography used for separation of hydroxycarbamide and urea |
| title_full | Hydrophylic interaction chromatography used for separation of hydroxycarbamide and urea |
| title_fullStr | Hydrophylic interaction chromatography used for separation of hydroxycarbamide and urea |
| title_full_unstemmed | Hydrophylic interaction chromatography used for separation of hydroxycarbamide and urea |
| title_short | Hydrophylic interaction chromatography used for separation of hydroxycarbamide and urea |
| title_sort | hydrophylic interaction chromatography used for separation of hydroxycarbamide and urea |
| topic | высокоэффективная жидкостная хроматография жидкостная хроматография гидрофильных взаимодействий гидроксикарбамид мочевина фармакопея high performance liquid chromatography hydrophilic interaction liquid chromatography hydroxycarbamide hydroxyurea pharmacopoeia |
| url | https://www.vedomostincesmp.ru/jour/article/view/123 |
| work_keys_str_mv | AT asosipov hydrophylicinteractionchromatographyusedforseparationofhydroxycarbamideandurea AT oapopova hydrophylicinteractionchromatographyusedforseparationofhydroxycarbamideandurea AT sglarionova hydrophylicinteractionchromatographyusedforseparationofhydroxycarbamideandurea AT eyutimoshina hydrophylicinteractionchromatographyusedforseparationofhydroxycarbamideandurea |