Functional Characterization, Genome Assembly, and Annotation of <i>Geobacillus</i> sp. G4 Isolated from a Geothermal Field in Tacna, Peru
The genome of <i>Geobacillus</i> sp. G4, a thermophilic bacterium isolated from a geothermal field in Peru, was sequenced and analyzed to evaluate its taxonomic and biotechnological potential. This strain exhibits optimal growth at temperatures between 50 and 70 °C and at a pH range of 6...
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2025-06-01
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| author | Alonso R. Poma Ticona Karita C. R. Santos Heber E. Ramirez-Arua Roberto Castellanos Jéssica Pinheiro Silva Pedro R. Vieira Hamann Eliane F. Noronha Fabyano A. C. Lopes |
| author_facet | Alonso R. Poma Ticona Karita C. R. Santos Heber E. Ramirez-Arua Roberto Castellanos Jéssica Pinheiro Silva Pedro R. Vieira Hamann Eliane F. Noronha Fabyano A. C. Lopes |
| author_sort | Alonso R. Poma Ticona |
| collection | DOAJ |
| description | The genome of <i>Geobacillus</i> sp. G4, a thermophilic bacterium isolated from a geothermal field in Peru, was sequenced and analyzed to evaluate its taxonomic and biotechnological potential. This strain exhibits optimal growth at temperatures between 50 and 70 °C and at a pH range of 6.0–7.5. Phenotypic assays demonstrated extracellular enzymatic activities, including amylases, cellulases, pectinases, and xylanases, highlighting its potential for efficient polysaccharide degradation. The assembled genome comprises approximately 3.4 Mb with a G+C content of 52.59%, containing 3,490 genes, including coding sequences, rRNAs, and tRNAs. Functional annotation revealed genes associated with key metabolic pathways such as glycogen and trehalose biosynthesis, indicating adaptation to carbohydrate-rich environments. Phylogenetic analyses based on ANI and dDDH values identified <i>Geobacillus thermoleovorans</i> KCTC3570 as its closest relative, suggesting a strong evolutionary relationship. Additionally, the genome harbors gene clusters for secondary metabolites such as betalactone and fengycin, suggesting potential industrial and pharmaceutical applications, including bioremediation. The identification of antibiotic resistance genes, specifically those conferring glycopeptide resistance, underscores their relevance for antimicrobial resistance studies. The presence of enzymes like amylases and pullulanase further emphasizes its biotechnological potential, particularly in starch hydrolysis and biofuel production. Overall, this research highlights the significant potential of <i>Geobacillus</i> species as valuable sources of thermostable enzymes and biosynthetic pathways for industrial applications. |
| format | Article |
| id | doaj-art-6b36ea8fce734046afe7cf4cbb4ad959 |
| institution | DOAJ |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-06-01 |
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| series | Microorganisms |
| spelling | doaj-art-6b36ea8fce734046afe7cf4cbb4ad9592025-08-20T03:16:35ZengMDPI AGMicroorganisms2076-26072025-06-01136137410.3390/microorganisms13061374Functional Characterization, Genome Assembly, and Annotation of <i>Geobacillus</i> sp. G4 Isolated from a Geothermal Field in Tacna, PeruAlonso R. Poma Ticona0Karita C. R. Santos1Heber E. Ramirez-Arua2Roberto Castellanos3Jéssica Pinheiro Silva4Pedro R. Vieira Hamann5Eliane F. Noronha6Fabyano A. C. Lopes7Enzyme Biotechnology Research Laboratory, Science Faculty, Universidad Nacional Jorge Basadre Grohmann, Tacna 23003, PeruLaboratório de Microbiologia, Universidade Federal Do Tocantins, Porto Nacional 77500-000, TO, BrazilLaboratorio de Biología Molecular, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima 15081, PeruEnzyme Biotechnology Research Laboratory, Science Faculty, Universidad Nacional Jorge Basadre Grohmann, Tacna 23003, PeruDepartment of Cell Biology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, DF, BrazilDepartment of Cell Biology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, DF, BrazilDepartment of Cell Biology, Institute of Biological Sciences, University of Brasilia, Brasilia 70910-900, DF, BrazilLaboratório de Microbiologia, Universidade Federal Do Tocantins, Porto Nacional 77500-000, TO, BrazilThe genome of <i>Geobacillus</i> sp. G4, a thermophilic bacterium isolated from a geothermal field in Peru, was sequenced and analyzed to evaluate its taxonomic and biotechnological potential. This strain exhibits optimal growth at temperatures between 50 and 70 °C and at a pH range of 6.0–7.5. Phenotypic assays demonstrated extracellular enzymatic activities, including amylases, cellulases, pectinases, and xylanases, highlighting its potential for efficient polysaccharide degradation. The assembled genome comprises approximately 3.4 Mb with a G+C content of 52.59%, containing 3,490 genes, including coding sequences, rRNAs, and tRNAs. Functional annotation revealed genes associated with key metabolic pathways such as glycogen and trehalose biosynthesis, indicating adaptation to carbohydrate-rich environments. Phylogenetic analyses based on ANI and dDDH values identified <i>Geobacillus thermoleovorans</i> KCTC3570 as its closest relative, suggesting a strong evolutionary relationship. Additionally, the genome harbors gene clusters for secondary metabolites such as betalactone and fengycin, suggesting potential industrial and pharmaceutical applications, including bioremediation. The identification of antibiotic resistance genes, specifically those conferring glycopeptide resistance, underscores their relevance for antimicrobial resistance studies. The presence of enzymes like amylases and pullulanase further emphasizes its biotechnological potential, particularly in starch hydrolysis and biofuel production. Overall, this research highlights the significant potential of <i>Geobacillus</i> species as valuable sources of thermostable enzymes and biosynthetic pathways for industrial applications.https://www.mdpi.com/2076-2607/13/6/1374bacterial thermophilybiotechnological potentialindustrial enzymesphylogenomic analysis |
| spellingShingle | Alonso R. Poma Ticona Karita C. R. Santos Heber E. Ramirez-Arua Roberto Castellanos Jéssica Pinheiro Silva Pedro R. Vieira Hamann Eliane F. Noronha Fabyano A. C. Lopes Functional Characterization, Genome Assembly, and Annotation of <i>Geobacillus</i> sp. G4 Isolated from a Geothermal Field in Tacna, Peru Microorganisms bacterial thermophily biotechnological potential industrial enzymes phylogenomic analysis |
| title | Functional Characterization, Genome Assembly, and Annotation of <i>Geobacillus</i> sp. G4 Isolated from a Geothermal Field in Tacna, Peru |
| title_full | Functional Characterization, Genome Assembly, and Annotation of <i>Geobacillus</i> sp. G4 Isolated from a Geothermal Field in Tacna, Peru |
| title_fullStr | Functional Characterization, Genome Assembly, and Annotation of <i>Geobacillus</i> sp. G4 Isolated from a Geothermal Field in Tacna, Peru |
| title_full_unstemmed | Functional Characterization, Genome Assembly, and Annotation of <i>Geobacillus</i> sp. G4 Isolated from a Geothermal Field in Tacna, Peru |
| title_short | Functional Characterization, Genome Assembly, and Annotation of <i>Geobacillus</i> sp. G4 Isolated from a Geothermal Field in Tacna, Peru |
| title_sort | functional characterization genome assembly and annotation of i geobacillus i sp g4 isolated from a geothermal field in tacna peru |
| topic | bacterial thermophily biotechnological potential industrial enzymes phylogenomic analysis |
| url | https://www.mdpi.com/2076-2607/13/6/1374 |
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