Farnesol inhibits translation to limit growth and filamentation in C. albicans and S. cerevisiae
Candida albicans is a polymorphic yeast where the capacity to switch between yeast and filamentous growth is critical for pathogenicity. Farnesol is a quorum-sensing sesquiterpene alcohol that, via regulation of specific signalling and transcription components, inhibits filamentous growth in C. albi...
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| Format: | Article |
| Language: | English |
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Shared Science Publishers OG
2017-09-01
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| Series: | Microbial Cell |
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| Online Access: | http://microbialcell.com/researcharticles/farnesol-inhibits-translation-to-limit-growth-and-filamentation-in-c-albicans-and-s-cerevisiae/ |
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| _version_ | 1850225509008408576 |
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| author | Nkechi E. Egbe Tawni O. Dornelles Caroline M. Paget Lydia M. Castelli Mark P. Ashe |
| author_facet | Nkechi E. Egbe Tawni O. Dornelles Caroline M. Paget Lydia M. Castelli Mark P. Ashe |
| author_sort | Nkechi E. Egbe |
| collection | DOAJ |
| description | Candida albicans is a polymorphic yeast where the capacity to switch between yeast and filamentous growth is critical for pathogenicity. Farnesol is a quorum-sensing sesquiterpene alcohol that, via regulation of specific signalling and transcription components, inhibits filamentous growth in C. albicans. Here we show that farnesol also inhibits translation at the initiation step in both C. albicans and S. cerevisiae. In contrast to fusel alcohols, that target the eukaryotic initiation factor 2B (eIF2B), farnesol affects the interaction of the mRNA with the small ribosomal subunit leading to reduced levels of the 48S preinitiation ribosomal complex in S. cerevisiae. Therefore, farnesol targets a different step in the translation pathway than fusel alcohols to elicit a completely opposite physiological outcome by negating filamentous growth. |
| format | Article |
| id | doaj-art-6b2a9f93a7e0418d908b4fee562a480b |
| institution | OA Journals |
| issn | 2311-2638 |
| language | English |
| publishDate | 2017-09-01 |
| publisher | Shared Science Publishers OG |
| record_format | Article |
| series | Microbial Cell |
| spelling | doaj-art-6b2a9f93a7e0418d908b4fee562a480b2025-08-20T02:05:20ZengShared Science Publishers OGMicrobial Cell2311-26382017-09-014929430410.15698/mic2017.09.589Farnesol inhibits translation to limit growth and filamentation in C. albicans and S. cerevisiaeNkechi E. Egbe0Tawni O. Dornelles1Caroline M. Paget2Lydia M. Castelli3Mark P. Ashe4Division of Molecular and Cellular Function, School of Biological Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Michael Smith Building, Oxford Rd., Manchester, M13 9PT, United Kingdom.Division of Molecular and Cellular Function, School of Biological Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Michael Smith Building, Oxford Rd., Manchester, M13 9PT, United Kingdom.Division of Molecular and Cellular Function, School of Biological Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Michael Smith Building, Oxford Rd., Manchester, M13 9PT, United Kingdom.Division of Molecular and Cellular Function, School of Biological Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Michael Smith Building, Oxford Rd., Manchester, M13 9PT, United Kingdom.Division of Molecular and Cellular Function, School of Biological Sciences, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Michael Smith Building, Oxford Rd., Manchester, M13 9PT, United Kingdom.Candida albicans is a polymorphic yeast where the capacity to switch between yeast and filamentous growth is critical for pathogenicity. Farnesol is a quorum-sensing sesquiterpene alcohol that, via regulation of specific signalling and transcription components, inhibits filamentous growth in C. albicans. Here we show that farnesol also inhibits translation at the initiation step in both C. albicans and S. cerevisiae. In contrast to fusel alcohols, that target the eukaryotic initiation factor 2B (eIF2B), farnesol affects the interaction of the mRNA with the small ribosomal subunit leading to reduced levels of the 48S preinitiation ribosomal complex in S. cerevisiae. Therefore, farnesol targets a different step in the translation pathway than fusel alcohols to elicit a completely opposite physiological outcome by negating filamentous growth.http://microbialcell.com/researcharticles/farnesol-inhibits-translation-to-limit-growth-and-filamentation-in-c-albicans-and-s-cerevisiae/protein synthesistranslation controlfarnesolquorum sensing |
| spellingShingle | Nkechi E. Egbe Tawni O. Dornelles Caroline M. Paget Lydia M. Castelli Mark P. Ashe Farnesol inhibits translation to limit growth and filamentation in C. albicans and S. cerevisiae Microbial Cell protein synthesis translation control farnesol quorum sensing |
| title | Farnesol inhibits translation to limit growth and filamentation in C. albicans and S. cerevisiae |
| title_full | Farnesol inhibits translation to limit growth and filamentation in C. albicans and S. cerevisiae |
| title_fullStr | Farnesol inhibits translation to limit growth and filamentation in C. albicans and S. cerevisiae |
| title_full_unstemmed | Farnesol inhibits translation to limit growth and filamentation in C. albicans and S. cerevisiae |
| title_short | Farnesol inhibits translation to limit growth and filamentation in C. albicans and S. cerevisiae |
| title_sort | farnesol inhibits translation to limit growth and filamentation in c albicans and s cerevisiae |
| topic | protein synthesis translation control farnesol quorum sensing |
| url | http://microbialcell.com/researcharticles/farnesol-inhibits-translation-to-limit-growth-and-filamentation-in-c-albicans-and-s-cerevisiae/ |
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