Amarogentin Displays Immunomodulatory Effects in Human Mast Cells and Keratinocytes
Keratinocytes express the bitter taste receptors TAS2R1 and TAS2R38. Amarogentin as an agonist for TAS2R1 and other TAS2Rs promotes keratinocyte differentiation. Similarly, mast cells are known to express bitter taste receptors. The aim of this study was to assess whether bitter compounds display im...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/630128 |
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| author | Ute Wölfle Birgit Haarhaus Christoph M. Schempp |
| author_facet | Ute Wölfle Birgit Haarhaus Christoph M. Schempp |
| author_sort | Ute Wölfle |
| collection | DOAJ |
| description | Keratinocytes express the bitter taste receptors TAS2R1 and TAS2R38. Amarogentin as an agonist for TAS2R1 and other TAS2Rs promotes keratinocyte differentiation. Similarly, mast cells are known to express bitter taste receptors. The aim of this study was to assess whether bitter compounds display immunomodulatory effects on these immunocompetent cells in the skin, so that they might be a target in chronic inflammatory diseases such as atopic dermatitis and psoriasis. Here, we investigated the impact of amarogentin on substance P-induced release of histamine and TNF-α from the human mast cell line LAD-2. Furthermore, the effect of amarogentin on HaCaT keratinocytes costimulated with TNF-α and histamine was investigated. Amarogentin inhibited in LAD-2 cells substance P-induced production of newly synthesized TNF-α, but the degranulation and release of stored histamine were not affected. In HaCaT keratinocytes histamine and TNF-α induced IL-8 and MMP-1 expression was reduced by amarogentin to a similar extent as with azelastine. In conclusion amarogentin displays immunomodulatory effects in the skin by interacting with mast cells and keratinocytes. |
| format | Article |
| id | doaj-art-6b2521af362743598e0e191dc5da9fe2 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-6b2521af362743598e0e191dc5da9fe22025-02-03T01:13:13ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/630128630128Amarogentin Displays Immunomodulatory Effects in Human Mast Cells and KeratinocytesUte Wölfle0Birgit Haarhaus1Christoph M. Schempp2Research Center skinitial, Department of Dermatology, University Medical Center, 79104 Freiburg, GermanyResearch Center skinitial, Department of Dermatology, University Medical Center, 79104 Freiburg, GermanyResearch Center skinitial, Department of Dermatology, University Medical Center, 79104 Freiburg, GermanyKeratinocytes express the bitter taste receptors TAS2R1 and TAS2R38. Amarogentin as an agonist for TAS2R1 and other TAS2Rs promotes keratinocyte differentiation. Similarly, mast cells are known to express bitter taste receptors. The aim of this study was to assess whether bitter compounds display immunomodulatory effects on these immunocompetent cells in the skin, so that they might be a target in chronic inflammatory diseases such as atopic dermatitis and psoriasis. Here, we investigated the impact of amarogentin on substance P-induced release of histamine and TNF-α from the human mast cell line LAD-2. Furthermore, the effect of amarogentin on HaCaT keratinocytes costimulated with TNF-α and histamine was investigated. Amarogentin inhibited in LAD-2 cells substance P-induced production of newly synthesized TNF-α, but the degranulation and release of stored histamine were not affected. In HaCaT keratinocytes histamine and TNF-α induced IL-8 and MMP-1 expression was reduced by amarogentin to a similar extent as with azelastine. In conclusion amarogentin displays immunomodulatory effects in the skin by interacting with mast cells and keratinocytes.http://dx.doi.org/10.1155/2015/630128 |
| spellingShingle | Ute Wölfle Birgit Haarhaus Christoph M. Schempp Amarogentin Displays Immunomodulatory Effects in Human Mast Cells and Keratinocytes Mediators of Inflammation |
| title | Amarogentin Displays Immunomodulatory Effects in Human Mast Cells and Keratinocytes |
| title_full | Amarogentin Displays Immunomodulatory Effects in Human Mast Cells and Keratinocytes |
| title_fullStr | Amarogentin Displays Immunomodulatory Effects in Human Mast Cells and Keratinocytes |
| title_full_unstemmed | Amarogentin Displays Immunomodulatory Effects in Human Mast Cells and Keratinocytes |
| title_short | Amarogentin Displays Immunomodulatory Effects in Human Mast Cells and Keratinocytes |
| title_sort | amarogentin displays immunomodulatory effects in human mast cells and keratinocytes |
| url | http://dx.doi.org/10.1155/2015/630128 |
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