The expression and significance of p53 protein and Ki-67 protein in pterygium
Background/Aim. Pterygium is considered to be a degenerative disease of the conjunctiva, however, the presence of tumor markers in pterygium reinforces the hypothesis that this lesion is similar to tumor. Inactivation of p53 function removes an obstacle to increased proliferation. Factors a...
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| Format: | Article |
| Language: | English |
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Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade
2016-01-01
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| Series: | Vojnosanitetski Pregled |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2016/0042-84501500124L.pdf |
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| _version_ | 1849413898602545152 |
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| author | Ljubojević Vesna Gajanin Radoslav Amidžić Ljiljana Vujković Zoran |
| author_facet | Ljubojević Vesna Gajanin Radoslav Amidžić Ljiljana Vujković Zoran |
| author_sort | Ljubojević Vesna |
| collection | DOAJ |
| description | Background/Aim. Pterygium is considered to be a degenerative disease of the
conjunctiva, however, the presence of tumor markers in pterygium reinforces
the hypothesis that this lesion is similar to tumor. Inactivation of p53
function removes an obstacle to increased proliferation. Factors affecting
the prevalence of p53 expression in pterygium deserve investigation. The
aim of the study was to investigate the expression of p53 and Ki-67 proteins
in pterygium and normal conjunctiva, the effects of gender and age on p53
expression, and the relationship between the expression of p53 and Ki-67
proteins. Methods. A total of 34 samples of pterygium and 34 samples of the
normal conjunctiva were analyzed. The samples were studied by
immunohistochemistry using antibodies against p53 and Ki-67. Results.
Totally 15 (44%) samples of pterygia were p53 positive. Correlations
between the expression of p53 protein and sex, and age were not established.
The number of Ki-67 positive cells in pterygium (9.74%) was significantly
higher than the number of Ki-67 positive cells in the normal conjunctiva
(1.74%), (p = 0.001). Between the expression of p53 protein and Ki-67
protein in pterygium there was a significant positive correlation (p =
0.000). Conclusion. The prevalence of p53 positive samples of pterygium was
44%. The influence of sex and age on p53 protein expression in pterygium was
not found. The increased proliferative acivity was present in the epithelium
of pterygium. The expression of Ki-67 protein is associated with the
expression of p53 protein in pterygium. The findings of our study support
the thesis of pterygium as tissue growth disorder. |
| format | Article |
| id | doaj-art-6b1c324d04ee45ccb36dd01b74ec292d |
| institution | Kabale University |
| issn | 0042-8450 2406-0720 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj-art-6b1c324d04ee45ccb36dd01b74ec292d2025-08-20T03:34:00ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502406-07202016-01-01731162010.2298/VSP140428124L0042-84501500124LThe expression and significance of p53 protein and Ki-67 protein in pterygiumLjubojević Vesna0Gajanin Radoslav1Amidžić Ljiljana2Vujković Zoran3University of Banja Luka, Faculty of Medicine, Banja Luka, Bosnia and Herzegovina + Clinical Center Banja Luka, Department of Histology and Embriology, Banja Luka, Bosnia and HerzegovinaUniversity of Banja Luka, Faculty of Medicine, Banja Luka, Bosnia and Herzegovina + Clinical Center Banja Luka, Department of Pathology, Banja Luka, Bosnia and HerzegovinaUniversity of Banja Luka, Faculty of Medicine, Banja Luka, Bosnia and Herzegovina + Clinical Center Banja Luka, Department of Histology and Embriology, Banja Luka, Bosnia and HerzegovinaUniversity of Banja Luka, Faculty of Medicine, Banja Luka, Bosnia and Herzegovina + Clinical Center Banja Luka, Clinic of Neurology, Banja Luka, Bosnia and HerzegovinaBackground/Aim. Pterygium is considered to be a degenerative disease of the conjunctiva, however, the presence of tumor markers in pterygium reinforces the hypothesis that this lesion is similar to tumor. Inactivation of p53 function removes an obstacle to increased proliferation. Factors affecting the prevalence of p53 expression in pterygium deserve investigation. The aim of the study was to investigate the expression of p53 and Ki-67 proteins in pterygium and normal conjunctiva, the effects of gender and age on p53 expression, and the relationship between the expression of p53 and Ki-67 proteins. Methods. A total of 34 samples of pterygium and 34 samples of the normal conjunctiva were analyzed. The samples were studied by immunohistochemistry using antibodies against p53 and Ki-67. Results. Totally 15 (44%) samples of pterygia were p53 positive. Correlations between the expression of p53 protein and sex, and age were not established. The number of Ki-67 positive cells in pterygium (9.74%) was significantly higher than the number of Ki-67 positive cells in the normal conjunctiva (1.74%), (p = 0.001). Between the expression of p53 protein and Ki-67 protein in pterygium there was a significant positive correlation (p = 0.000). Conclusion. The prevalence of p53 positive samples of pterygium was 44%. The influence of sex and age on p53 protein expression in pterygium was not found. The increased proliferative acivity was present in the epithelium of pterygium. The expression of Ki-67 protein is associated with the expression of p53 protein in pterygium. The findings of our study support the thesis of pterygium as tissue growth disorder.http://www.doiserbia.nb.rs/img/doi/0042-8450/2016/0042-84501500124L.pdfpterygiumtumor markers, biologicaltumor suppressor protein p53conjunctivaage factosseximmunohistochemistry |
| spellingShingle | Ljubojević Vesna Gajanin Radoslav Amidžić Ljiljana Vujković Zoran The expression and significance of p53 protein and Ki-67 protein in pterygium Vojnosanitetski Pregled pterygium tumor markers, biological tumor suppressor protein p53 conjunctiva age factos sex immunohistochemistry |
| title | The expression and significance of p53 protein and Ki-67 protein in pterygium |
| title_full | The expression and significance of p53 protein and Ki-67 protein in pterygium |
| title_fullStr | The expression and significance of p53 protein and Ki-67 protein in pterygium |
| title_full_unstemmed | The expression and significance of p53 protein and Ki-67 protein in pterygium |
| title_short | The expression and significance of p53 protein and Ki-67 protein in pterygium |
| title_sort | expression and significance of p53 protein and ki 67 protein in pterygium |
| topic | pterygium tumor markers, biological tumor suppressor protein p53 conjunctiva age factos sex immunohistochemistry |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2016/0042-84501500124L.pdf |
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