Biological effects of pyropheophorbide-a as photosensitizer and prooxidant
UV-visible absorption, chemiluminescence and ESR (electron spin-resonance) were employed to investigate characteristics of pyropheophorbide-a. The results showed that pyropheophorbide-a was photosensitized and prooxidated as the way of model Ⅱ; By estimating the viability of Tn-5B1-4 cells and morta...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2007-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2007.06.0641 |
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| Summary: | UV-visible absorption, chemiluminescence and ESR (electron spin-resonance) were employed to investigate characteristics of pyropheophorbide-a. The results showed that pyropheophorbide-a was photosensitized and prooxidated as the way of model Ⅱ; By estimating the viability of Tn-5B1-4 cells and mortality of third instar larvae of Plutella xylostella L. under illumination, it was found that pyropheophorbide-a significantly influenced on the viability of Tn-5B1-4 cells and the mortality of third instar larvae of P. xylostella L. The cell viability of Tn-5B1-4 was reduced to 6.29%, disposed with pyropheophorbide-a for 48 h with 30 min light irradiation at the concentration of 10 μmol·L<sup>-1</sup>. After fed with pyropheophorbide-a 48 h with irradiation of ultraviolet and sunlight for 1 h, the death rate of third instar larvae of P. xylostella L., was 60% and 100%, respectively, significantly different with control. It was indicated that pyropheophorbide-a is an excellent photosensitizer and prooxidant in biological applications. |
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| ISSN: | 1008-9209 2097-5155 |