Subcellular Localization of a Glycoprotein Released from Human Platelets upon Stimulation by Thrombin
The treatment of washed, intact human blood platelets with 1 U/ml of thrombin for 5 min at 37 °C is linked to the disappearance from the particulate fraction of the homogenized cells of a protein band discernible in the SDS-polyacrylamide gel electrophoresis pattern of untreated platelets. Accordin...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | deu |
| Published: |
Swiss Chemical Society
1976-02-01
|
| Series: | CHIMIA |
| Online Access: | https://www.chimia.ch/chimia/article/view/9291 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850283820823085056 |
|---|---|
| author | R. Käser-Glanzmann M. Jakábová E.F. Lüscher |
| author_facet | R. Käser-Glanzmann M. Jakábová E.F. Lüscher |
| author_sort | R. Käser-Glanzmann |
| collection | DOAJ |
| description |
The treatment of washed, intact human blood platelets with 1 U/ml of thrombin for 5 min at 37 °C is linked to the disappearance from the particulate fraction of the homogenized cells of a protein band discernible in the SDS-polyacrylamide gel electrophoresis pattern of untreated platelets. Accordingly, this material was termed thrombin sensitive protein (TSP) by its discoverers, who also presented evidence that TSP is a glycoprotein of a molecular weight of 190,000.
The present work describes the localization of TSP in human platelets. TSP is not a membrane constituent, but associated with orgenelle fractions in the region of higher densities. It therefore is not, as originally suggested, a direct substrate for thrombin on the platelet surface, but takes part in the platelet release reaction upon a variety of external stimuli. An apparent molecular weight of 150,000 was found for TSP; this value coincides with the one reported for so-called glycoprotein I, which, different from TSP, is a membrane constituent.
|
| format | Article |
| id | doaj-art-6b13ae3487f5410abdf8a21db40c22ce |
| institution | OA Journals |
| issn | 0009-4293 2673-2424 |
| language | deu |
| publishDate | 1976-02-01 |
| publisher | Swiss Chemical Society |
| record_format | Article |
| series | CHIMIA |
| spelling | doaj-art-6b13ae3487f5410abdf8a21db40c22ce2025-08-20T01:47:42ZdeuSwiss Chemical SocietyCHIMIA0009-42932673-24241976-02-0130210.2533/chimia.1976.96Subcellular Localization of a Glycoprotein Released from Human Platelets upon Stimulation by ThrombinR. Käser-Glanzmann0M. Jakábová1E.F. Lüscher2Theodor-Kocher-Institut, Universität BernTheodor-Kocher-Institut, Universität BernTheodor-Kocher-Institut, Universität Bern The treatment of washed, intact human blood platelets with 1 U/ml of thrombin for 5 min at 37 °C is linked to the disappearance from the particulate fraction of the homogenized cells of a protein band discernible in the SDS-polyacrylamide gel electrophoresis pattern of untreated platelets. Accordingly, this material was termed thrombin sensitive protein (TSP) by its discoverers, who also presented evidence that TSP is a glycoprotein of a molecular weight of 190,000. The present work describes the localization of TSP in human platelets. TSP is not a membrane constituent, but associated with orgenelle fractions in the region of higher densities. It therefore is not, as originally suggested, a direct substrate for thrombin on the platelet surface, but takes part in the platelet release reaction upon a variety of external stimuli. An apparent molecular weight of 150,000 was found for TSP; this value coincides with the one reported for so-called glycoprotein I, which, different from TSP, is a membrane constituent. https://www.chimia.ch/chimia/article/view/9291 |
| spellingShingle | R. Käser-Glanzmann M. Jakábová E.F. Lüscher Subcellular Localization of a Glycoprotein Released from Human Platelets upon Stimulation by Thrombin CHIMIA |
| title | Subcellular Localization of a Glycoprotein Released from Human Platelets upon Stimulation by Thrombin |
| title_full | Subcellular Localization of a Glycoprotein Released from Human Platelets upon Stimulation by Thrombin |
| title_fullStr | Subcellular Localization of a Glycoprotein Released from Human Platelets upon Stimulation by Thrombin |
| title_full_unstemmed | Subcellular Localization of a Glycoprotein Released from Human Platelets upon Stimulation by Thrombin |
| title_short | Subcellular Localization of a Glycoprotein Released from Human Platelets upon Stimulation by Thrombin |
| title_sort | subcellular localization of a glycoprotein released from human platelets upon stimulation by thrombin |
| url | https://www.chimia.ch/chimia/article/view/9291 |
| work_keys_str_mv | AT rkaserglanzmann subcellularlocalizationofaglycoproteinreleasedfromhumanplateletsuponstimulationbythrombin AT mjakabova subcellularlocalizationofaglycoproteinreleasedfromhumanplateletsuponstimulationbythrombin AT efluscher subcellularlocalizationofaglycoproteinreleasedfromhumanplateletsuponstimulationbythrombin |