Genome Mining-Guided Discovery of Two New Depsides from <i>Talaromyces</i> sp. HDN1820200
Depsides and their derivatives are a class of polyketides predominantly found in fungal extracts. Herein, a silent nonreducing polyketide synthase (TalsA)-containing gene cluster, which was identified from the Antarctic sponge-derived fungus <i>Talaromyces</i> sp. HDN1820200, was success...
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2025-03-01
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| author | Xiao Zhang Luyang Liu Jiani Huang Xingtao Ren Guojian Zhang Qian Che Dehai Li Tianjiao Zhu |
| author_facet | Xiao Zhang Luyang Liu Jiani Huang Xingtao Ren Guojian Zhang Qian Che Dehai Li Tianjiao Zhu |
| author_sort | Xiao Zhang |
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| description | Depsides and their derivatives are a class of polyketides predominantly found in fungal extracts. Herein, a silent nonreducing polyketide synthase (TalsA)-containing gene cluster, which was identified from the Antarctic sponge-derived fungus <i>Talaromyces</i> sp. HDN1820200, was successfully activated through heterologous expression in <i>Aspergillus nidulans</i>. This activation led to the production of two novel depsides, talaronic acid A (<b>1</b>) and B (<b>2</b>), alongside three known compounds (<b>3</b>–<b>5</b>). The further co-expression of TalsA with the decarboxylase (TalsF) demonstrated that it could convert <b>2</b> into its decarboxylated derivative <b>1</b>. The structural elucidation of these compounds was achieved using comprehensive 1D and 2D-NMR spectroscopy, which was complemented by HR-MS analysis. Talaronic acids A and B were firstly reported heterodimers of 3-methylorsellinic acid (3-MOA) and 5-methylorsellinic acid (5-MOA). All isolated compounds (<b>1</b>–<b>5</b>) were tested for their anti-inflammatory potential. Notably, compounds <b>1</b> and <b>2</b> exhibited anti-inflammatory activity comparable to that of the positive control. These results further enrich the structural class of depside natural products. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-03-01 |
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| spelling | doaj-art-6b12d8b1bf96413e81742ed3dee910232025-08-20T03:43:27ZengMDPI AGMarine Drugs1660-33972025-03-0123313010.3390/md23030130Genome Mining-Guided Discovery of Two New Depsides from <i>Talaromyces</i> sp. HDN1820200Xiao Zhang0Luyang Liu1Jiani Huang2Xingtao Ren3Guojian Zhang4Qian Che5Dehai Li6Tianjiao Zhu7Key Laboratory of Marine Drugs Ministry of Education, Ocean University of China, Qingdao 266003, ChinaKey Laboratory of Marine Drugs Ministry of Education, Ocean University of China, Qingdao 266003, ChinaKey Laboratory of Marine Drugs Ministry of Education, Ocean University of China, Qingdao 266003, ChinaKey Laboratory of Marine Drugs Ministry of Education, Ocean University of China, Qingdao 266003, ChinaKey Laboratory of Marine Drugs Ministry of Education, Ocean University of China, Qingdao 266003, ChinaKey Laboratory of Marine Drugs Ministry of Education, Ocean University of China, Qingdao 266003, ChinaKey Laboratory of Marine Drugs Ministry of Education, Ocean University of China, Qingdao 266003, ChinaKey Laboratory of Marine Drugs Ministry of Education, Ocean University of China, Qingdao 266003, ChinaDepsides and their derivatives are a class of polyketides predominantly found in fungal extracts. Herein, a silent nonreducing polyketide synthase (TalsA)-containing gene cluster, which was identified from the Antarctic sponge-derived fungus <i>Talaromyces</i> sp. HDN1820200, was successfully activated through heterologous expression in <i>Aspergillus nidulans</i>. This activation led to the production of two novel depsides, talaronic acid A (<b>1</b>) and B (<b>2</b>), alongside three known compounds (<b>3</b>–<b>5</b>). The further co-expression of TalsA with the decarboxylase (TalsF) demonstrated that it could convert <b>2</b> into its decarboxylated derivative <b>1</b>. The structural elucidation of these compounds was achieved using comprehensive 1D and 2D-NMR spectroscopy, which was complemented by HR-MS analysis. Talaronic acids A and B were firstly reported heterodimers of 3-methylorsellinic acid (3-MOA) and 5-methylorsellinic acid (5-MOA). All isolated compounds (<b>1</b>–<b>5</b>) were tested for their anti-inflammatory potential. Notably, compounds <b>1</b> and <b>2</b> exhibited anti-inflammatory activity comparable to that of the positive control. These results further enrich the structural class of depside natural products.https://www.mdpi.com/1660-3397/23/3/130depsides<i>Talaromyces</i> sp.heterodimeranti-inflammatory effects |
| spellingShingle | Xiao Zhang Luyang Liu Jiani Huang Xingtao Ren Guojian Zhang Qian Che Dehai Li Tianjiao Zhu Genome Mining-Guided Discovery of Two New Depsides from <i>Talaromyces</i> sp. HDN1820200 Marine Drugs depsides <i>Talaromyces</i> sp. heterodimer anti-inflammatory effects |
| title | Genome Mining-Guided Discovery of Two New Depsides from <i>Talaromyces</i> sp. HDN1820200 |
| title_full | Genome Mining-Guided Discovery of Two New Depsides from <i>Talaromyces</i> sp. HDN1820200 |
| title_fullStr | Genome Mining-Guided Discovery of Two New Depsides from <i>Talaromyces</i> sp. HDN1820200 |
| title_full_unstemmed | Genome Mining-Guided Discovery of Two New Depsides from <i>Talaromyces</i> sp. HDN1820200 |
| title_short | Genome Mining-Guided Discovery of Two New Depsides from <i>Talaromyces</i> sp. HDN1820200 |
| title_sort | genome mining guided discovery of two new depsides from i talaromyces i sp hdn1820200 |
| topic | depsides <i>Talaromyces</i> sp. heterodimer anti-inflammatory effects |
| url | https://www.mdpi.com/1660-3397/23/3/130 |
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