Dietary supplementation of microbiota inoculum and single clove garlic extract on growth performance, egg quality, reproductive organ, and hematological trait in laying quail

Background: Several alternative feed additives to replace AGP to maintain good quail performance include the use of probiotics and herbal extracts. Aim: In this study, the researchers want to find out the best dosage of microbiota inoculum as probiotics (Lactobacillus acidophilus, Bifidobacteriu...

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Main Authors: Bodhi Agustono, Maya Nurwartanti Yunita, Widya Paramita Lokapirnasari, Sunaryo Hadi Warsito, Tabita Dameria Marbun, Sarasati Windria
Format: Article
Language:English
Published: Tripoli University 2025-02-01
Series:Open Veterinary Journal
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Online Access:http://www.ejmanager.com/fulltextpdf.php?mno=225079
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author Bodhi Agustono
Maya Nurwartanti Yunita
Widya Paramita Lokapirnasari
Sunaryo Hadi Warsito
Tabita Dameria Marbun
Sarasati Windria
author_facet Bodhi Agustono
Maya Nurwartanti Yunita
Widya Paramita Lokapirnasari
Sunaryo Hadi Warsito
Tabita Dameria Marbun
Sarasati Windria
author_sort Bodhi Agustono
collection DOAJ
description Background: Several alternative feed additives to replace AGP to maintain good quail performance include the use of probiotics and herbal extracts. Aim: In this study, the researchers want to find out the best dosage of microbiota inoculum as probiotics (Lactobacillus acidophilus, Bifidobacterium, and Lactobacillus plantarum), single garlic extract (Allium Sativum L), and combination groups to improve laying quail egg production, haematology profile (Erythrocytes, Haemoglobin, Haematocrit, Leukocytes, and Platelets), and reproductive organs in terms of length and weight and have better egg quality on the internal (haugh units, yolk score, albumin index, yolk index) and external (shell thickness, egg weight) while laying phase of laying quail. Methods: A total of 100 4-week-old laying quails of uniform body weight were randomly distributed into five treatments with four replicates each and five quails in each replicate. There were 5 treatment groups: T0 quails were given basal feed; T1 quails were given basal feed and drinking water added with probiotics at a dose of 4 ml/L; T2 quails were given feed added with a single garlic extract at a dose of 2 ml/g and ordinary drinking water; T3 quails were given feed with a single garlic extract at a dose of 2 ml/g and drinking water supplemented with probiotics at a dose of 4 ml/L; and T4 quails were given a single garlic extract at a dose of 1 ml/g and drinking water supplemented with probiotics at a dose of 2 ml/L. The study investigated the effects of microbiota inoculum, including probiotics, garlic extract, and a control group, on reproductive organ morphologies in chickens. Results showed significant improvements in weight vagina, uterus, oviduct, ovarium, weight cloaca, growth performance, body weight, egg production, internal egg quality, yolk color score, yolk index, albumin index, external egg quality, haugh unit, egg height, shape index, eggcell weight, and eggcell thickness. Results: The number of erythrocytes was significantly higher in the chickens treated with the microbiota inoculum compared to the control group. Haemoglobin levels were not significant in all treatments, but haematocrit levels were significant in the chickens treated with the microbiota inoculum. Leukocytes were also significantly higher in the chickens treated with the microbiota inoculum compared to the control group. Conclusion: The use of a combination of microbiota inoculum in drinking water and garlic extract in feed has been proven to be effective in reducing feed consumption, maintaining hematology, increasing reproductive organs, and boosting the number of laying quail productions, thereby reducing Feed Conversion Ratio. [Open Vet J 2025; 15(2.000): 690-699]
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spelling doaj-art-6b0d4167c2d64ca6a2daf54ee12f75f62025-08-20T02:11:55ZengTripoli UniversityOpen Veterinary Journal2226-44852218-60502025-02-0115269069910.5455/OVJ.2025.v15.i2.17225079Dietary supplementation of microbiota inoculum and single clove garlic extract on growth performance, egg quality, reproductive organ, and hematological trait in laying quailBodhi Agustono0Maya Nurwartanti Yunita1Widya Paramita Lokapirnasari2Sunaryo Hadi Warsito3Tabita Dameria Marbun4Sarasati Windria5Doctoral Program Veterinary of Science, Faculty of Veterinary, Airlangga University Faculty of Health, Medicine and Life Sciences, Universitas Airlangga, Surabaya 60115, Indonesia Division of Animal Husbandry, Department of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia Faculty of Health, Medicine and Life Sciences, Universitas Airlangga, Surabaya 60115, Indonesia Division of Animal Husbandry, Department of Veterinary Science, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia Department of Biomedical Science, Faculty of Medicine, Universitas Padjajaran, Bandung, IndonesiaBackground: Several alternative feed additives to replace AGP to maintain good quail performance include the use of probiotics and herbal extracts. Aim: In this study, the researchers want to find out the best dosage of microbiota inoculum as probiotics (Lactobacillus acidophilus, Bifidobacterium, and Lactobacillus plantarum), single garlic extract (Allium Sativum L), and combination groups to improve laying quail egg production, haematology profile (Erythrocytes, Haemoglobin, Haematocrit, Leukocytes, and Platelets), and reproductive organs in terms of length and weight and have better egg quality on the internal (haugh units, yolk score, albumin index, yolk index) and external (shell thickness, egg weight) while laying phase of laying quail. Methods: A total of 100 4-week-old laying quails of uniform body weight were randomly distributed into five treatments with four replicates each and five quails in each replicate. There were 5 treatment groups: T0 quails were given basal feed; T1 quails were given basal feed and drinking water added with probiotics at a dose of 4 ml/L; T2 quails were given feed added with a single garlic extract at a dose of 2 ml/g and ordinary drinking water; T3 quails were given feed with a single garlic extract at a dose of 2 ml/g and drinking water supplemented with probiotics at a dose of 4 ml/L; and T4 quails were given a single garlic extract at a dose of 1 ml/g and drinking water supplemented with probiotics at a dose of 2 ml/L. The study investigated the effects of microbiota inoculum, including probiotics, garlic extract, and a control group, on reproductive organ morphologies in chickens. Results showed significant improvements in weight vagina, uterus, oviduct, ovarium, weight cloaca, growth performance, body weight, egg production, internal egg quality, yolk color score, yolk index, albumin index, external egg quality, haugh unit, egg height, shape index, eggcell weight, and eggcell thickness. Results: The number of erythrocytes was significantly higher in the chickens treated with the microbiota inoculum compared to the control group. Haemoglobin levels were not significant in all treatments, but haematocrit levels were significant in the chickens treated with the microbiota inoculum. Leukocytes were also significantly higher in the chickens treated with the microbiota inoculum compared to the control group. Conclusion: The use of a combination of microbiota inoculum in drinking water and garlic extract in feed has been proven to be effective in reducing feed consumption, maintaining hematology, increasing reproductive organs, and boosting the number of laying quail productions, thereby reducing Feed Conversion Ratio. [Open Vet J 2025; 15(2.000): 690-699]http://www.ejmanager.com/fulltextpdf.php?mno=225079probioticsgood healthgrowth performancequality eggsreproductive organsquail
spellingShingle Bodhi Agustono
Maya Nurwartanti Yunita
Widya Paramita Lokapirnasari
Sunaryo Hadi Warsito
Tabita Dameria Marbun
Sarasati Windria
Dietary supplementation of microbiota inoculum and single clove garlic extract on growth performance, egg quality, reproductive organ, and hematological trait in laying quail
Open Veterinary Journal
probiotics
good health
growth performance
quality eggs
reproductive organs
quail
title Dietary supplementation of microbiota inoculum and single clove garlic extract on growth performance, egg quality, reproductive organ, and hematological trait in laying quail
title_full Dietary supplementation of microbiota inoculum and single clove garlic extract on growth performance, egg quality, reproductive organ, and hematological trait in laying quail
title_fullStr Dietary supplementation of microbiota inoculum and single clove garlic extract on growth performance, egg quality, reproductive organ, and hematological trait in laying quail
title_full_unstemmed Dietary supplementation of microbiota inoculum and single clove garlic extract on growth performance, egg quality, reproductive organ, and hematological trait in laying quail
title_short Dietary supplementation of microbiota inoculum and single clove garlic extract on growth performance, egg quality, reproductive organ, and hematological trait in laying quail
title_sort dietary supplementation of microbiota inoculum and single clove garlic extract on growth performance egg quality reproductive organ and hematological trait in laying quail
topic probiotics
good health
growth performance
quality eggs
reproductive organs
quail
url http://www.ejmanager.com/fulltextpdf.php?mno=225079
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