Development of NGR-GelMA Hydrogels for PC3 Prostate Cancer Cells
Prostate cancer is one of the most common cancer for men. Current therapies such as chemotherapy or radiotherapy non-spesifically affect cancerous cells. Current therapies need more targeted delivery approaches such as peptide. Asn-Gly-Arg (NGR) is a tool for cancer targeting therapy. To mimic more...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sakarya University
2024-02-01
|
| Series: | Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/download/article-file/3316466 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850100051546734592 |
|---|---|
| author | Ozan Karaman Meryem Zeybekoğlu Ziyşan Buse Yaralı Çevik |
| author_facet | Ozan Karaman Meryem Zeybekoğlu Ziyşan Buse Yaralı Çevik |
| author_sort | Ozan Karaman |
| collection | DOAJ |
| description | Prostate cancer is one of the most common cancer for men. Current therapies such as chemotherapy or radiotherapy non-spesifically affect cancerous cells. Current therapies need more targeted delivery approaches such as peptide. Asn-Gly-Arg (NGR) is a tool for cancer targeting therapy. To mimic more natural cancer microenvironment, peptide treatment approaches are examined in 3 Dimensional (D) hydrogels. GelMA is one of the hydrogels that permits to construct 3D microenvironment of PC3 prostate cancer cells. The goal of the study was to evaluate characteristic of GelMA to model prostate cancer environment and to determine the effects of NGR peptides for PC3 line. pH values of different concentrations NGR (1 µM, 10 µM and 100 µM)-GelMA were measured. To analyze biodegradation capacity of different concentrations NGR (1 µM, 10 µM and 100 µM)-GelMA, weigth measurements were performed. Live and Dead analysis was performed on days 1, 4, and 7. The findings revealed that GelMA hydrogels created a relatively stable and neutral pH, making them potentially valuable for drug delivery systems. Furthermore, the NGR-GelMA hydrogels incorporated exhibited the capacity to absorb liquids, resulting in an increase in weight. Notably, these hydrogels allowed for the observation of the dynamic 3D microenvironment of prostate cancer, which was influenced by the concentration of the targeted drug in the GelMA matrix. This suggests promising implications for developing targeted therapies for prostate cancer using GelMA-based drug delivery systems. As a conclusion, GelMA and NGR-GelMA hyrdogels may be useful platform for further studies to progress on prostate cancer treatment. |
| format | Article |
| id | doaj-art-6b0845b58e9d438d8bb810ca87c986b4 |
| institution | DOAJ |
| issn | 2147-835X |
| language | English |
| publishDate | 2024-02-01 |
| publisher | Sakarya University |
| record_format | Article |
| series | Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi |
| spelling | doaj-art-6b0845b58e9d438d8bb810ca87c986b42025-08-20T02:40:23ZengSakarya UniversitySakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi2147-835X2024-02-0128119520310.16984/saufenbilder.133966028Development of NGR-GelMA Hydrogels for PC3 Prostate Cancer CellsOzan Karaman0https://orcid.org/0000-0002-4175-4402Meryem Zeybekoğlu1https://orcid.org/0009-0004-8232-9551Ziyşan Buse Yaralı Çevik2https://orcid.org/0000-0002-9371-6424IZMIR KATIP CELEBI UNIVERSITY, FACULTY OF ENGINEERING-ARCHITECTURE, DEPARTMENT OF BIOMEDICAL ENGINEERINGIZMIR KATIP CELEBI UNIVERSITY, FACULTY OF ENGINEERING-ARCHITECTURE, DEPARTMENT OF BIOMEDICAL ENGINEERING, BIOMEDICAL ENGINEERING PR. (ENGLISH)İZMİR KATİP ÇELEBİ ÜNİVERSİTESİProstate cancer is one of the most common cancer for men. Current therapies such as chemotherapy or radiotherapy non-spesifically affect cancerous cells. Current therapies need more targeted delivery approaches such as peptide. Asn-Gly-Arg (NGR) is a tool for cancer targeting therapy. To mimic more natural cancer microenvironment, peptide treatment approaches are examined in 3 Dimensional (D) hydrogels. GelMA is one of the hydrogels that permits to construct 3D microenvironment of PC3 prostate cancer cells. The goal of the study was to evaluate characteristic of GelMA to model prostate cancer environment and to determine the effects of NGR peptides for PC3 line. pH values of different concentrations NGR (1 µM, 10 µM and 100 µM)-GelMA were measured. To analyze biodegradation capacity of different concentrations NGR (1 µM, 10 µM and 100 µM)-GelMA, weigth measurements were performed. Live and Dead analysis was performed on days 1, 4, and 7. The findings revealed that GelMA hydrogels created a relatively stable and neutral pH, making them potentially valuable for drug delivery systems. Furthermore, the NGR-GelMA hydrogels incorporated exhibited the capacity to absorb liquids, resulting in an increase in weight. Notably, these hydrogels allowed for the observation of the dynamic 3D microenvironment of prostate cancer, which was influenced by the concentration of the targeted drug in the GelMA matrix. This suggests promising implications for developing targeted therapies for prostate cancer using GelMA-based drug delivery systems. As a conclusion, GelMA and NGR-GelMA hyrdogels may be useful platform for further studies to progress on prostate cancer treatment.https://dergipark.org.tr/tr/download/article-file/3316466prostate cancergelmangr3 dimensional (3d) |
| spellingShingle | Ozan Karaman Meryem Zeybekoğlu Ziyşan Buse Yaralı Çevik Development of NGR-GelMA Hydrogels for PC3 Prostate Cancer Cells Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi prostate cancer gelma ngr 3 dimensional (3d) |
| title | Development of NGR-GelMA Hydrogels for PC3 Prostate Cancer Cells |
| title_full | Development of NGR-GelMA Hydrogels for PC3 Prostate Cancer Cells |
| title_fullStr | Development of NGR-GelMA Hydrogels for PC3 Prostate Cancer Cells |
| title_full_unstemmed | Development of NGR-GelMA Hydrogels for PC3 Prostate Cancer Cells |
| title_short | Development of NGR-GelMA Hydrogels for PC3 Prostate Cancer Cells |
| title_sort | development of ngr gelma hydrogels for pc3 prostate cancer cells |
| topic | prostate cancer gelma ngr 3 dimensional (3d) |
| url | https://dergipark.org.tr/tr/download/article-file/3316466 |
| work_keys_str_mv | AT ozankaraman developmentofngrgelmahydrogelsforpc3prostatecancercells AT meryemzeybekoglu developmentofngrgelmahydrogelsforpc3prostatecancercells AT ziysanbuseyaralıcevik developmentofngrgelmahydrogelsforpc3prostatecancercells |