Protocol for measuring D-amino acid transport in mammalian cells using a fluorescent biosensor
Summary: The movement of amino acids into cells and organelles is facilitated by transporter proteins, which support homeostasis, metabolism, and disease progression. Here, we present a protocol to visualize amino acid transport in mammalian cells in real time using an enzyme-based biosensor. We pro...
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| Main Authors: | Danisha Johal, Keeley G. Hewton, Sadie J. Gale, Amritpal S. Johal, Seth J. Parker |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725003351 |
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