CRISPR/cas9 Allows for the Quick Improvement of Tomato Firmness Breeding
Fruit firmness is crucial for storability, making cultivating varieties with higher firmness a key target in tomato breeding. In recent years, tomato varieties primarily rely on hybridizing ripening mutants to produce F<sub>1</sub> hybrids to enhance firmness. However, the undesirable tr...
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MDPI AG
2024-12-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/47/1/9 |
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| author | Qihong Yang Liangyu Cai Mila Wang Guiyun Gan Weiliu Li Wenjia Li Yaqin Jiang Qi Yuan Chunchun Qin Chuying Yu Yikui Wang |
| author_facet | Qihong Yang Liangyu Cai Mila Wang Guiyun Gan Weiliu Li Wenjia Li Yaqin Jiang Qi Yuan Chunchun Qin Chuying Yu Yikui Wang |
| author_sort | Qihong Yang |
| collection | DOAJ |
| description | Fruit firmness is crucial for storability, making cultivating varieties with higher firmness a key target in tomato breeding. In recent years, tomato varieties primarily rely on hybridizing ripening mutants to produce F<sub>1</sub> hybrids to enhance firmness. However, the undesirable traits introduced by these mutants often lead to a decline in the quality of the varieties. CRISPR/Cas9 has emerged as a crucial tool in accelerating plant breeding and improving specific target traits as technology iterates. In this study, we used a CRISPR/Cas9 system to simultaneously knock out two genes, <i>FIS1</i> and <i>PL</i>, which negatively regulate firmness in tomato. We generated single and double gene knockout mutants utilizing the tomato genetic transformation system. The fruit firmness of all knockout mutants exhibited a significant enhancement, with the most pronounced improvement observed in the double mutant. Furthermore, we assessed other quality-related traits of the mutants; our results indicated that the fruit quality characteristics of the gene-edited lines remained statistically comparable to those of the wild type. This approach enabled us to create transgenic-free mutants with diverse genotypes across fewer generations, facilitating rapid improvements in tomato firmness. This study offers significant insights into molecular design breeding strategies for tomato. |
| format | Article |
| id | doaj-art-6afc87dd99c04a04be7b2869ab7c83c9 |
| institution | Kabale University |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj-art-6afc87dd99c04a04be7b2869ab7c83c92025-01-24T13:27:23ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-12-01471910.3390/cimb47010009CRISPR/cas9 Allows for the Quick Improvement of Tomato Firmness BreedingQihong Yang0Liangyu Cai1Mila Wang2Guiyun Gan3Weiliu Li4Wenjia Li5Yaqin Jiang6Qi Yuan7Chunchun Qin8Chuying Yu9Yikui Wang10Vegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaVegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaFruit firmness is crucial for storability, making cultivating varieties with higher firmness a key target in tomato breeding. In recent years, tomato varieties primarily rely on hybridizing ripening mutants to produce F<sub>1</sub> hybrids to enhance firmness. However, the undesirable traits introduced by these mutants often lead to a decline in the quality of the varieties. CRISPR/Cas9 has emerged as a crucial tool in accelerating plant breeding and improving specific target traits as technology iterates. In this study, we used a CRISPR/Cas9 system to simultaneously knock out two genes, <i>FIS1</i> and <i>PL</i>, which negatively regulate firmness in tomato. We generated single and double gene knockout mutants utilizing the tomato genetic transformation system. The fruit firmness of all knockout mutants exhibited a significant enhancement, with the most pronounced improvement observed in the double mutant. Furthermore, we assessed other quality-related traits of the mutants; our results indicated that the fruit quality characteristics of the gene-edited lines remained statistically comparable to those of the wild type. This approach enabled us to create transgenic-free mutants with diverse genotypes across fewer generations, facilitating rapid improvements in tomato firmness. This study offers significant insights into molecular design breeding strategies for tomato.https://www.mdpi.com/1467-3045/47/1/9tomatoCRISPR/cas9fruit firmnessgermplasm improvementtransgenic-free |
| spellingShingle | Qihong Yang Liangyu Cai Mila Wang Guiyun Gan Weiliu Li Wenjia Li Yaqin Jiang Qi Yuan Chunchun Qin Chuying Yu Yikui Wang CRISPR/cas9 Allows for the Quick Improvement of Tomato Firmness Breeding Current Issues in Molecular Biology tomato CRISPR/cas9 fruit firmness germplasm improvement transgenic-free |
| title | CRISPR/cas9 Allows for the Quick Improvement of Tomato Firmness Breeding |
| title_full | CRISPR/cas9 Allows for the Quick Improvement of Tomato Firmness Breeding |
| title_fullStr | CRISPR/cas9 Allows for the Quick Improvement of Tomato Firmness Breeding |
| title_full_unstemmed | CRISPR/cas9 Allows for the Quick Improvement of Tomato Firmness Breeding |
| title_short | CRISPR/cas9 Allows for the Quick Improvement of Tomato Firmness Breeding |
| title_sort | crispr cas9 allows for the quick improvement of tomato firmness breeding |
| topic | tomato CRISPR/cas9 fruit firmness germplasm improvement transgenic-free |
| url | https://www.mdpi.com/1467-3045/47/1/9 |
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