The Dose Rate of Corpuscular Ionizing Radiation Strongly Influences the Severity of DNA Damage, Cell Cycle Progression and Cellular Senescence in Human Epidermoid Carcinoma Cells
Modern radiotherapy utilizes a broad range of sources of ionizing radiation, both low-dose-rate (LDR) and high-dose-rate (HDR). However, the mechanisms underlying specific dose-rate effects remain unclear, especially for corpuscular radiation. To address this issue, we have irradiated human epidermo...
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2024-12-01
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| author | Sergey S. Soroko Dmitry V. Skamnitskiy Ekaterina N. Gorshkova Olga M. Kutova Ismail R. Seriev Anna V. Maslennikova Evgeniy L. Guryev Sergey V. Gudkov Vladimir A. Vodeneev Irina V. Balalaeva Natalia Yu Shilyagina |
| author_facet | Sergey S. Soroko Dmitry V. Skamnitskiy Ekaterina N. Gorshkova Olga M. Kutova Ismail R. Seriev Anna V. Maslennikova Evgeniy L. Guryev Sergey V. Gudkov Vladimir A. Vodeneev Irina V. Balalaeva Natalia Yu Shilyagina |
| author_sort | Sergey S. Soroko |
| collection | DOAJ |
| description | Modern radiotherapy utilizes a broad range of sources of ionizing radiation, both low-dose-rate (LDR) and high-dose-rate (HDR). However, the mechanisms underlying specific dose-rate effects remain unclear, especially for corpuscular radiation. To address this issue, we have irradiated human epidermoid carcinoma A431 cells under LDR and HDR regimes. Reducing the dose rate has lower lethality at equal doses with HDR irradiation. The half-lethal dose after HDR irradiation was three times less than after LDR irradiation. The study of mechanisms showed that under HDR irradiation, the radiation-induced halt of mitosis with the accompanying emergence of giant cells was recorded. No such changes were recorded after LDR irradiation. The level of DNA damage is significantly greater after HDR irradiation, which may be the main reason for the different mechanisms of action of HDR and LDR irradiations. Comparing the mechanisms of cell response to LDR and HDR irradiations may shed light on the mechanisms of tumor cell response to ionizing radiation and answer the question of whether different dose rates within the same dose range can cause different clinical effects. |
| format | Article |
| id | doaj-art-6afc2ac1bf004aa38a714a3c639eecb2 |
| institution | Kabale University |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj-art-6afc2ac1bf004aa38a714a3c639eecb22024-12-27T14:18:14ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-12-014612138601388010.3390/cimb46120828The Dose Rate of Corpuscular Ionizing Radiation Strongly Influences the Severity of DNA Damage, Cell Cycle Progression and Cellular Senescence in Human Epidermoid Carcinoma CellsSergey S. Soroko0Dmitry V. Skamnitskiy1Ekaterina N. Gorshkova2Olga M. Kutova3Ismail R. Seriev4Anna V. Maslennikova5Evgeniy L. Guryev6Sergey V. Gudkov7Vladimir A. Vodeneev8Irina V. Balalaeva9Natalia Yu Shilyagina10Department of Biophysics, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave., 603022 Nizhny Novgorod, RussiaNizhniy Novgorod Regional Oncology Hospital, St. Rodionova, 190, 603950 Nizhny Novgorod, RussiaDepartment of Biophysics, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave., 603022 Nizhny Novgorod, RussiaDepartment of Biophysics, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave., 603022 Nizhny Novgorod, RussiaDepartment of Biophysics, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave., 603022 Nizhny Novgorod, RussiaDepartment of Biophysics, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave., 603022 Nizhny Novgorod, RussiaDepartment of Biophysics, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave., 603022 Nizhny Novgorod, RussiaDepartment of Biophysics, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave., 603022 Nizhny Novgorod, RussiaDepartment of Biophysics, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave., 603022 Nizhny Novgorod, RussiaDepartment of Biophysics, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave., 603022 Nizhny Novgorod, RussiaDepartment of Biophysics, Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Ave., 603022 Nizhny Novgorod, RussiaModern radiotherapy utilizes a broad range of sources of ionizing radiation, both low-dose-rate (LDR) and high-dose-rate (HDR). However, the mechanisms underlying specific dose-rate effects remain unclear, especially for corpuscular radiation. To address this issue, we have irradiated human epidermoid carcinoma A431 cells under LDR and HDR regimes. Reducing the dose rate has lower lethality at equal doses with HDR irradiation. The half-lethal dose after HDR irradiation was three times less than after LDR irradiation. The study of mechanisms showed that under HDR irradiation, the radiation-induced halt of mitosis with the accompanying emergence of giant cells was recorded. No such changes were recorded after LDR irradiation. The level of DNA damage is significantly greater after HDR irradiation, which may be the main reason for the different mechanisms of action of HDR and LDR irradiations. Comparing the mechanisms of cell response to LDR and HDR irradiations may shed light on the mechanisms of tumor cell response to ionizing radiation and answer the question of whether different dose rates within the same dose range can cause different clinical effects.https://www.mdpi.com/1467-3045/46/12/828ionizing radiationradiotherapycancerlow dose ratehigh dose ratemechanism cell response |
| spellingShingle | Sergey S. Soroko Dmitry V. Skamnitskiy Ekaterina N. Gorshkova Olga M. Kutova Ismail R. Seriev Anna V. Maslennikova Evgeniy L. Guryev Sergey V. Gudkov Vladimir A. Vodeneev Irina V. Balalaeva Natalia Yu Shilyagina The Dose Rate of Corpuscular Ionizing Radiation Strongly Influences the Severity of DNA Damage, Cell Cycle Progression and Cellular Senescence in Human Epidermoid Carcinoma Cells Current Issues in Molecular Biology ionizing radiation radiotherapy cancer low dose rate high dose rate mechanism cell response |
| title | The Dose Rate of Corpuscular Ionizing Radiation Strongly Influences the Severity of DNA Damage, Cell Cycle Progression and Cellular Senescence in Human Epidermoid Carcinoma Cells |
| title_full | The Dose Rate of Corpuscular Ionizing Radiation Strongly Influences the Severity of DNA Damage, Cell Cycle Progression and Cellular Senescence in Human Epidermoid Carcinoma Cells |
| title_fullStr | The Dose Rate of Corpuscular Ionizing Radiation Strongly Influences the Severity of DNA Damage, Cell Cycle Progression and Cellular Senescence in Human Epidermoid Carcinoma Cells |
| title_full_unstemmed | The Dose Rate of Corpuscular Ionizing Radiation Strongly Influences the Severity of DNA Damage, Cell Cycle Progression and Cellular Senescence in Human Epidermoid Carcinoma Cells |
| title_short | The Dose Rate of Corpuscular Ionizing Radiation Strongly Influences the Severity of DNA Damage, Cell Cycle Progression and Cellular Senescence in Human Epidermoid Carcinoma Cells |
| title_sort | dose rate of corpuscular ionizing radiation strongly influences the severity of dna damage cell cycle progression and cellular senescence in human epidermoid carcinoma cells |
| topic | ionizing radiation radiotherapy cancer low dose rate high dose rate mechanism cell response |
| url | https://www.mdpi.com/1467-3045/46/12/828 |
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