Serum concentrations of per- and poly-fluoroalkyl substances (PFAS) in Danish pregnant women—temporal trends during pregnancy, correlations with partners, associations with physical activity, and blood lipid concentrations

Abstract Background Per- and polyflouralkyl substances (PFAS) are a group of persistent chemicals used extensively in industries and consumer products due to their water-repellent properties. Studies have linked PFAS exposure to adverse health effects, and human exposure to PFAS, especially during p...

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Main Authors: Ida Karoline Bach Jensen, Esben Budtz-Jørgensen, Christian Lindh, Caroline Borup Roland, Signe de Place Knudsen, Jane M. Bendix, Stig Molsted, Tine D. Clausen, Bente Stallknecht, Ole H. Mortensen, Ellen Løkkegaard, Lisbeth E. Knudsen
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Language:English
Published: BMC 2025-04-01
Series:Environmental Health
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Online Access:https://doi.org/10.1186/s12940-025-01170-x
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author Ida Karoline Bach Jensen
Esben Budtz-Jørgensen
Christian Lindh
Caroline Borup Roland
Signe de Place Knudsen
Jane M. Bendix
Stig Molsted
Tine D. Clausen
Bente Stallknecht
Ole H. Mortensen
Ellen Løkkegaard
Lisbeth E. Knudsen
author_facet Ida Karoline Bach Jensen
Esben Budtz-Jørgensen
Christian Lindh
Caroline Borup Roland
Signe de Place Knudsen
Jane M. Bendix
Stig Molsted
Tine D. Clausen
Bente Stallknecht
Ole H. Mortensen
Ellen Løkkegaard
Lisbeth E. Knudsen
author_sort Ida Karoline Bach Jensen
collection DOAJ
description Abstract Background Per- and polyflouralkyl substances (PFAS) are a group of persistent chemicals used extensively in industries and consumer products due to their water-repellent properties. Studies have linked PFAS exposure to adverse health effects, and human exposure to PFAS, especially during pregnancy, is of great concern. In this study, we report how serum PFAS concentrations during pregnancy correlated with serum PFAS of partners from the same household. Further, we report how serum PFAS concentrations change during the course of pregnancy and associations between PFAS and blood lipid concentrations as well as exploratory analyses of associations between physical activity and PFAS concentrations. Methods In this secondary analysis of data from the FitMum study conducted from 2018 to 2021, 216 healthy, pregnant women, and 110 of their partners were included. Non-fasting venous blood samples were collected from the mothers at three test visits during pregnancy and at delivery, where blood from partners were also collected. Serum samples from all timepoints were analyzed for 15 short- and long-chained PFAS using liquid chromatography triple quadrupole linear ion trap mass spectrometry. Total cholesterol, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and triglyceride blood concentrations were measured at three test visits during pregnancy and at delivery. Physical activity was measured with a wrist-worn activity tracker 24/7 from inclusion before gestational age week 15 + 0 and throughout pregnancy. Results In serum samples we detected the following PFAS: PFOS, PFOA, PFHxS, PFNA, PFDA, and PFUnDA. The maternal median concentrations at baseline were: PFOS: 4.09 ng/mL, PFOA: 0.81 ng/mL, PFHxS: 0.29 ng/mL, PFNA: 0.42 ng/mL, PFDA: 0.25 ng/mL, and PFUnDA: 0.19 ng/mL. Partner serum PFAS concentrations were 3–145% higher than maternal concentration (except for PFUnDA). PFAS concentrations correlated within couples. All PFAS decreased significantly during pregnancy (PFOS -23.1 percent 95%-CI [-31.9;-13.2] from baseline to delivery). All PFAS concentrations were associated with increased HDL-C concentrations. No associations between physical activity and maternal PFAS concentrations were found. Conclusions Overall, serum PFAS concentrations decreased during pregnancy. PFAS concentrations within households were strongly correlated. PFAS and HDL-C concentrations were positively associated. We found no associations between physical activity and serum PFAS concentrations. Trial registration The study was registered at ClinicalTrials.gov; NCT03679130; 20/09/2018.
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spelling doaj-art-6aeb60d124fc473496ce37538de751ca2025-08-20T01:56:09ZengBMCEnvironmental Health1476-069X2025-04-0124111310.1186/s12940-025-01170-xSerum concentrations of per- and poly-fluoroalkyl substances (PFAS) in Danish pregnant women—temporal trends during pregnancy, correlations with partners, associations with physical activity, and blood lipid concentrationsIda Karoline Bach Jensen0Esben Budtz-Jørgensen1Christian Lindh2Caroline Borup Roland3Signe de Place Knudsen4Jane M. Bendix5Stig Molsted6Tine D. Clausen7Bente Stallknecht8Ole H. Mortensen9Ellen Løkkegaard10Lisbeth E. Knudsen11Department of Gynecology and Obstetrics, Copenhagen University Hospital - North ZealandDepartment of Public Health, Section of Biostatistics, University of CopenhagenDivision of Occupational and Environmental Medicine, Department of Laboratory Medicine, Lund UniversityDepartment of Gynecology and Obstetrics, Copenhagen University Hospital - North ZealandDepartment of Gynecology and Obstetrics, Copenhagen University Hospital - North ZealandDepartment of Gynecology and Obstetrics, Copenhagen University Hospital - North ZealandDepartment of Clinical Research, Copenhagen University Hospital - North ZealandDepartment of Gynecology and Obstetrics, Copenhagen University Hospital - North ZealandDepartment of Biomedical Sciences, University of CopenhagenDepartment of Biomedical Sciences, University of CopenhagenDepartment of Gynecology and Obstetrics, Copenhagen University Hospital - North ZealandDepartment of Public Health, Section of Environmental Health, University of CopenhagenAbstract Background Per- and polyflouralkyl substances (PFAS) are a group of persistent chemicals used extensively in industries and consumer products due to their water-repellent properties. Studies have linked PFAS exposure to adverse health effects, and human exposure to PFAS, especially during pregnancy, is of great concern. In this study, we report how serum PFAS concentrations during pregnancy correlated with serum PFAS of partners from the same household. Further, we report how serum PFAS concentrations change during the course of pregnancy and associations between PFAS and blood lipid concentrations as well as exploratory analyses of associations between physical activity and PFAS concentrations. Methods In this secondary analysis of data from the FitMum study conducted from 2018 to 2021, 216 healthy, pregnant women, and 110 of their partners were included. Non-fasting venous blood samples were collected from the mothers at three test visits during pregnancy and at delivery, where blood from partners were also collected. Serum samples from all timepoints were analyzed for 15 short- and long-chained PFAS using liquid chromatography triple quadrupole linear ion trap mass spectrometry. Total cholesterol, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and triglyceride blood concentrations were measured at three test visits during pregnancy and at delivery. Physical activity was measured with a wrist-worn activity tracker 24/7 from inclusion before gestational age week 15 + 0 and throughout pregnancy. Results In serum samples we detected the following PFAS: PFOS, PFOA, PFHxS, PFNA, PFDA, and PFUnDA. The maternal median concentrations at baseline were: PFOS: 4.09 ng/mL, PFOA: 0.81 ng/mL, PFHxS: 0.29 ng/mL, PFNA: 0.42 ng/mL, PFDA: 0.25 ng/mL, and PFUnDA: 0.19 ng/mL. Partner serum PFAS concentrations were 3–145% higher than maternal concentration (except for PFUnDA). PFAS concentrations correlated within couples. All PFAS decreased significantly during pregnancy (PFOS -23.1 percent 95%-CI [-31.9;-13.2] from baseline to delivery). All PFAS concentrations were associated with increased HDL-C concentrations. No associations between physical activity and maternal PFAS concentrations were found. Conclusions Overall, serum PFAS concentrations decreased during pregnancy. PFAS concentrations within households were strongly correlated. PFAS and HDL-C concentrations were positively associated. We found no associations between physical activity and serum PFAS concentrations. Trial registration The study was registered at ClinicalTrials.gov; NCT03679130; 20/09/2018.https://doi.org/10.1186/s12940-025-01170-xBiomonitoringPregnancyPerfluorinated substancesPolyflouroalkyl substancesEnvironmetal chemicalsPhysical activity
spellingShingle Ida Karoline Bach Jensen
Esben Budtz-Jørgensen
Christian Lindh
Caroline Borup Roland
Signe de Place Knudsen
Jane M. Bendix
Stig Molsted
Tine D. Clausen
Bente Stallknecht
Ole H. Mortensen
Ellen Løkkegaard
Lisbeth E. Knudsen
Serum concentrations of per- and poly-fluoroalkyl substances (PFAS) in Danish pregnant women—temporal trends during pregnancy, correlations with partners, associations with physical activity, and blood lipid concentrations
Environmental Health
Biomonitoring
Pregnancy
Perfluorinated substances
Polyflouroalkyl substances
Environmetal chemicals
Physical activity
title Serum concentrations of per- and poly-fluoroalkyl substances (PFAS) in Danish pregnant women—temporal trends during pregnancy, correlations with partners, associations with physical activity, and blood lipid concentrations
title_full Serum concentrations of per- and poly-fluoroalkyl substances (PFAS) in Danish pregnant women—temporal trends during pregnancy, correlations with partners, associations with physical activity, and blood lipid concentrations
title_fullStr Serum concentrations of per- and poly-fluoroalkyl substances (PFAS) in Danish pregnant women—temporal trends during pregnancy, correlations with partners, associations with physical activity, and blood lipid concentrations
title_full_unstemmed Serum concentrations of per- and poly-fluoroalkyl substances (PFAS) in Danish pregnant women—temporal trends during pregnancy, correlations with partners, associations with physical activity, and blood lipid concentrations
title_short Serum concentrations of per- and poly-fluoroalkyl substances (PFAS) in Danish pregnant women—temporal trends during pregnancy, correlations with partners, associations with physical activity, and blood lipid concentrations
title_sort serum concentrations of per and poly fluoroalkyl substances pfas in danish pregnant women temporal trends during pregnancy correlations with partners associations with physical activity and blood lipid concentrations
topic Biomonitoring
Pregnancy
Perfluorinated substances
Polyflouroalkyl substances
Environmetal chemicals
Physical activity
url https://doi.org/10.1186/s12940-025-01170-x
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