Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke
Currently, there is increasing interest in human bone marrow stromal cells (hBMSCs) as regeneration therapy against cerebral stroke. The aim of the present study was to evaluate the feasibility and validity of hBMSC cultures with allogeneic platelet lysates (PLs). Platelet concentrates (PC) were har...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/6104780 |
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| author | Chengbo Tan Hideo Shichinohe Zifeng Wang Shuji Hamauchi Takeo Abumiya Naoki Nakayama Ken Kazumata Tsuneo Ito Kohsuke Kudo Shigeru Takamoto Kiyohiro Houkin |
| author_facet | Chengbo Tan Hideo Shichinohe Zifeng Wang Shuji Hamauchi Takeo Abumiya Naoki Nakayama Ken Kazumata Tsuneo Ito Kohsuke Kudo Shigeru Takamoto Kiyohiro Houkin |
| author_sort | Chengbo Tan |
| collection | DOAJ |
| description | Currently, there is increasing interest in human bone marrow stromal cells (hBMSCs) as regeneration therapy against cerebral stroke. The aim of the present study was to evaluate the feasibility and validity of hBMSC cultures with allogeneic platelet lysates (PLs). Platelet concentrates (PC) were harvested from healthy volunteers and made into single donor-derived PL (sPL). The PL mixtures (mPL) were made from three different sPL. Some growth factors and platelet cell surface antigens were detected by enzyme-linked immunosorbent assay (ELISA). The hBMSCs cultured with 10% PL were analyzed for their proliferative potential, surface markers, and karyotypes. The cells were incubated with superparamagnetic iron oxide (SPIO) agents and injected into a pig brain. MRI and histological analysis were performed. Consequently, nine lots of sPL and three mPL were prepared. ELISA analysis showed that PL contained adequate growth factors and a particle of platelet surface antigens. Cell proliferation capacity of PLs was equivalent to or higher than that of fetal calf serum (FCS). No contradiction in cell surface markers and no chromosomal aberrations were found. The MRI detected the distribution of SPIO-labeled hBMSCs in the pig brain. In summary, the hBMSCs cultured with allogeneic PL are suitable for cell therapy against stroke. |
| format | Article |
| id | doaj-art-6ae5e44d5f424efea9e20e3dba6694a0 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-6ae5e44d5f424efea9e20e3dba6694a02025-02-03T06:10:56ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/61047806104780Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against StrokeChengbo Tan0Hideo Shichinohe1Zifeng Wang2Shuji Hamauchi3Takeo Abumiya4Naoki Nakayama5Ken Kazumata6Tsuneo Ito7Kohsuke Kudo8Shigeru Takamoto9Kiyohiro Houkin10Department of Neurosurgery, Hokkaido University Graduate School of Medicine, Sapporo, JapanDepartment of Neurosurgery, Hokkaido University Graduate School of Medicine, Sapporo, JapanDepartment of Neurosurgery, Hokkaido University Graduate School of Medicine, Sapporo, JapanDepartment of Neurosurgery, Hokkaido University Graduate School of Medicine, Sapporo, JapanDepartment of Neurosurgery, Hokkaido University Graduate School of Medicine, Sapporo, JapanDepartment of Neurosurgery, Hokkaido University Graduate School of Medicine, Sapporo, JapanDepartment of Neurosurgery, Hokkaido University Graduate School of Medicine, Sapporo, JapanHokkaido University Hospital Clinical Research and Medical Innovation Center, Sapporo, JapanDepartment of Radiology, Hokkaido University Graduate School of Medicine, Sapporo, JapanJapanese Red Cross, Hokkaido Block Blood Center, Sapporo, JapanDepartment of Neurosurgery, Hokkaido University Graduate School of Medicine, Sapporo, JapanCurrently, there is increasing interest in human bone marrow stromal cells (hBMSCs) as regeneration therapy against cerebral stroke. The aim of the present study was to evaluate the feasibility and validity of hBMSC cultures with allogeneic platelet lysates (PLs). Platelet concentrates (PC) were harvested from healthy volunteers and made into single donor-derived PL (sPL). The PL mixtures (mPL) were made from three different sPL. Some growth factors and platelet cell surface antigens were detected by enzyme-linked immunosorbent assay (ELISA). The hBMSCs cultured with 10% PL were analyzed for their proliferative potential, surface markers, and karyotypes. The cells were incubated with superparamagnetic iron oxide (SPIO) agents and injected into a pig brain. MRI and histological analysis were performed. Consequently, nine lots of sPL and three mPL were prepared. ELISA analysis showed that PL contained adequate growth factors and a particle of platelet surface antigens. Cell proliferation capacity of PLs was equivalent to or higher than that of fetal calf serum (FCS). No contradiction in cell surface markers and no chromosomal aberrations were found. The MRI detected the distribution of SPIO-labeled hBMSCs in the pig brain. In summary, the hBMSCs cultured with allogeneic PL are suitable for cell therapy against stroke.http://dx.doi.org/10.1155/2016/6104780 |
| spellingShingle | Chengbo Tan Hideo Shichinohe Zifeng Wang Shuji Hamauchi Takeo Abumiya Naoki Nakayama Ken Kazumata Tsuneo Ito Kohsuke Kudo Shigeru Takamoto Kiyohiro Houkin Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke Stem Cells International |
| title | Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke |
| title_full | Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke |
| title_fullStr | Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke |
| title_full_unstemmed | Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke |
| title_short | Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke |
| title_sort | feasibility and efficiency of human bone marrow stromal cell culture with allogeneic platelet lysate supplementation for cell therapy against stroke |
| url | http://dx.doi.org/10.1155/2016/6104780 |
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