Baicalin mitigates alcoholic-associated liver disease via SOCS1-driven reprogramming of macrophages

Abstract Backgrounds Alcoholic liver disease (ALD), a consequence of excessive alcohol consumption, is characterized by high incidence and mortality rates. Presently, there are no effective pharmacological interventions available for the treatment of ALD. Baicalin (BA), a natural flavonoid derived f...

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Main Authors: Sha Huang, Yuhua Wang, Jinjie Wen, Wenjuan Ji, Qiuxiang Zeng, Kaili Deng, Min Li, Shanshan Kuang, Wen Zhang, Mo Chan, Chuying Zhou, Zhiping Lv, Shaohui Huang
Format: Article
Language:English
Published: BMC 2025-05-01
Series:Chinese Medicine
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Online Access:https://doi.org/10.1186/s13020-025-01110-4
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author Sha Huang
Yuhua Wang
Jinjie Wen
Wenjuan Ji
Qiuxiang Zeng
Kaili Deng
Min Li
Shanshan Kuang
Wen Zhang
Mo Chan
Chuying Zhou
Zhiping Lv
Shaohui Huang
author_facet Sha Huang
Yuhua Wang
Jinjie Wen
Wenjuan Ji
Qiuxiang Zeng
Kaili Deng
Min Li
Shanshan Kuang
Wen Zhang
Mo Chan
Chuying Zhou
Zhiping Lv
Shaohui Huang
author_sort Sha Huang
collection DOAJ
description Abstract Backgrounds Alcoholic liver disease (ALD), a consequence of excessive alcohol consumption, is characterized by high incidence and mortality rates. Presently, there are no effective pharmacological interventions available for the treatment of ALD. Baicalin (BA), a natural flavonoid derived from the root of Scutellaria baicalensis, has exhibited notable hepatoprotective effects. Nevertheless, the mechanisms through which BA influences the interaction between suppressor of cytokine signaling 1 (SOCS1) and macrophages during hepatic immune development remain insufficiently understood. Materials and methods This study seeks to examine the regulatory effects of BA on ALD and to elucidate the relationship between SOCS1 and macrophage differentiation. Our experimental methodology involves the novel application of zebrafish as an in vivo model for ALD. To further investigate the underlying mechanisms, we employed gene knockout and overexpression techniques. Results The study demonstrates that BA substantially alleviates ALD in both in vivo and in vitro settings by upregulating SOCS1 expression in macrophages. Furthermore, we elucidated the association between SOCS1 and macrophage reprogramming. Specifically, SOCS1 knockdown led to the downregulation of CD86, CD80, and iNOS expression, whereas SOCS1 overexpression enhanced the expression of CD206, CD163, IL-4, and IL-10. Conclusion In conclusion, our findings suggest that BA attenuates ALD via the modulation of SOCS1-mediated macrophage reprogramming.
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spelling doaj-art-6ad75f7e702f4c52a4b6cc3da1ef42db2025-08-20T01:51:36ZengBMCChinese Medicine1749-85462025-05-0120111510.1186/s13020-025-01110-4Baicalin mitigates alcoholic-associated liver disease via SOCS1-driven reprogramming of macrophagesSha Huang0Yuhua Wang1Jinjie Wen2Wenjuan Ji3Qiuxiang Zeng4Kaili Deng5Min Li6Shanshan Kuang7Wen Zhang8Mo Chan9Chuying Zhou10Zhiping Lv11Shaohui Huang12School of Traditional Chinese Medicine, Southern Medical UniversitySchool of Traditional Chinese Medicine, Southern Medical UniversitySchool of Traditional Chinese Medicine, Southern Medical UniversitySchool of Traditional Chinese Medicine, Southern Medical UniversitySchool of Traditional Chinese Medicine, Southern Medical UniversitySchool of Traditional Chinese Medicine, Southern Medical UniversitySchool of Traditional Chinese Medicine, Southern Medical UniversitySchool of Traditional Chinese Medicine, Southern Medical UniversitySchool of Traditional Chinese Medicine, Southern Medical UniversityDepartment of First Clinical Medical College, Guangdong Pharmaceutical UniversitySchool of Traditional Chinese Medicine, Southern Medical UniversitySchool of Traditional Chinese Medicine, Southern Medical UniversitySchool of Traditional Chinese Medicine, Southern Medical UniversityAbstract Backgrounds Alcoholic liver disease (ALD), a consequence of excessive alcohol consumption, is characterized by high incidence and mortality rates. Presently, there are no effective pharmacological interventions available for the treatment of ALD. Baicalin (BA), a natural flavonoid derived from the root of Scutellaria baicalensis, has exhibited notable hepatoprotective effects. Nevertheless, the mechanisms through which BA influences the interaction between suppressor of cytokine signaling 1 (SOCS1) and macrophages during hepatic immune development remain insufficiently understood. Materials and methods This study seeks to examine the regulatory effects of BA on ALD and to elucidate the relationship between SOCS1 and macrophage differentiation. Our experimental methodology involves the novel application of zebrafish as an in vivo model for ALD. To further investigate the underlying mechanisms, we employed gene knockout and overexpression techniques. Results The study demonstrates that BA substantially alleviates ALD in both in vivo and in vitro settings by upregulating SOCS1 expression in macrophages. Furthermore, we elucidated the association between SOCS1 and macrophage reprogramming. Specifically, SOCS1 knockdown led to the downregulation of CD86, CD80, and iNOS expression, whereas SOCS1 overexpression enhanced the expression of CD206, CD163, IL-4, and IL-10. Conclusion In conclusion, our findings suggest that BA attenuates ALD via the modulation of SOCS1-mediated macrophage reprogramming.https://doi.org/10.1186/s13020-025-01110-4ALDBaicalinSOCS1MacrophageReprogramming
spellingShingle Sha Huang
Yuhua Wang
Jinjie Wen
Wenjuan Ji
Qiuxiang Zeng
Kaili Deng
Min Li
Shanshan Kuang
Wen Zhang
Mo Chan
Chuying Zhou
Zhiping Lv
Shaohui Huang
Baicalin mitigates alcoholic-associated liver disease via SOCS1-driven reprogramming of macrophages
Chinese Medicine
ALD
Baicalin
SOCS1
Macrophage
Reprogramming
title Baicalin mitigates alcoholic-associated liver disease via SOCS1-driven reprogramming of macrophages
title_full Baicalin mitigates alcoholic-associated liver disease via SOCS1-driven reprogramming of macrophages
title_fullStr Baicalin mitigates alcoholic-associated liver disease via SOCS1-driven reprogramming of macrophages
title_full_unstemmed Baicalin mitigates alcoholic-associated liver disease via SOCS1-driven reprogramming of macrophages
title_short Baicalin mitigates alcoholic-associated liver disease via SOCS1-driven reprogramming of macrophages
title_sort baicalin mitigates alcoholic associated liver disease via socs1 driven reprogramming of macrophages
topic ALD
Baicalin
SOCS1
Macrophage
Reprogramming
url https://doi.org/10.1186/s13020-025-01110-4
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