Effects of dietary fish meal replacement by Periplaneta americana meal on growth, metabolism, intestinal health and resistance against Aeromonas hydrophila of Micropterus salmoides

An 8-week culture experiment was conducted to investigate the effects of partial replacement of fish meal (FM) with Periplaneta americana meal (PAM) on growth performance, body composition, serum biochemical parameters, intestinal health and resistance against Aeromonas hydrophila of largemouth bass...

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Main Authors: Yuanyi Liu, Changchang Pu, Zihui Wei, Yang Wang, Weichuan Zhang, Hongyu Chen, Yong Huang
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Aquaculture Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S2352513424005337
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author Yuanyi Liu
Changchang Pu
Zihui Wei
Yang Wang
Weichuan Zhang
Hongyu Chen
Yong Huang
author_facet Yuanyi Liu
Changchang Pu
Zihui Wei
Yang Wang
Weichuan Zhang
Hongyu Chen
Yong Huang
author_sort Yuanyi Liu
collection DOAJ
description An 8-week culture experiment was conducted to investigate the effects of partial replacement of fish meal (FM) with Periplaneta americana meal (PAM) on growth performance, body composition, serum biochemical parameters, intestinal health and resistance against Aeromonas hydrophila of largemouth bass, Micropterus salmoides. A total of 450 healthy largemouth bass with an initial body weight (IBW) of 14.55±0.09 g were randomly divided into five groups. Five experimental diets were prepared by replacing 0 % (P0), 15 % (P15), 30 % (P30), 45 % (P45) and 60 % (P60) of the FM in the basal diet with PAM, respectively. The results showed that final body weight (FBW), weight gain rate (WGR), and specific growth rate (SGR) were no significant difference in P15 and P30 compared with the control group (P > 0.05). Crude protein of whole fish body was significantly increased and crude lipid was significantly decreased in all PAM groups (P < 0.05). In the P30, the antioxidant enzyme activities were significantly higher than those in the control and other experimental groups, and malondialdehyde (MDA) was significantly decreased (P < 0.05). The activities of α-amylase and pepsin increased significantly in P15 and P30 and decreased significantly in P45 and P60 (P < 0.05). Lipase was significantly increased in all groups except P15 than in the control group (P < 0.05). There were no significant differences in villus height (VH), crypt depth (CD), and muscle thickness (MT) among all groups (P > 0.05), but villus width (VW) was significantly decreased in P45 and P60 (P < 0.05). The relative expression of IL-10 and TGF-β1 were first up-regulated and then down-regulated, while IL-8 was opposite (P < 0.05). The relative expression of Casp3, Casp9 and IFN-γ were significantly up-regulated in P45 and P60 (P < 0.05). After injecting A. hydrophila, all groups of largemouth bass experienced mortality, but cumulative mortality was lowest in the P30. In conclusion, PAM can partially replace FM in the diet of juvenile largemouth bass. The replacement of no more than 30 % is unlikely to adversely affect growth performance, and can increase the crude protein content of the whole fish body, and is beneficial to intestinal health.
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spelling doaj-art-6acae95d88214e54a17c392b204f97be2025-08-20T02:51:31ZengElsevierAquaculture Reports2352-51342024-12-013910244510.1016/j.aqrep.2024.102445Effects of dietary fish meal replacement by Periplaneta americana meal on growth, metabolism, intestinal health and resistance against Aeromonas hydrophila of Micropterus salmoidesYuanyi Liu0Changchang Pu1Zihui Wei2Yang Wang3Weichuan Zhang4Hongyu Chen5Yong Huang6Animal Science and Technology College, Henan University of Science and Technology, Luoyang 471003, ChinaAnimal Science and Technology College, Henan University of Science and Technology, Luoyang 471003, ChinaAnimal Science and Technology College, Henan University of Science and Technology, Luoyang 471003, ChinaAnimal Science and Technology College, Henan University of Science and Technology, Luoyang 471003, ChinaAnimal Science and Technology College, Henan University of Science and Technology, Luoyang 471003, ChinaAnimal Science and Technology College, Henan University of Science and Technology, Luoyang 471003, ChinaCorresponding author.; Animal Science and Technology College, Henan University of Science and Technology, Luoyang 471003, ChinaAn 8-week culture experiment was conducted to investigate the effects of partial replacement of fish meal (FM) with Periplaneta americana meal (PAM) on growth performance, body composition, serum biochemical parameters, intestinal health and resistance against Aeromonas hydrophila of largemouth bass, Micropterus salmoides. A total of 450 healthy largemouth bass with an initial body weight (IBW) of 14.55±0.09 g were randomly divided into five groups. Five experimental diets were prepared by replacing 0 % (P0), 15 % (P15), 30 % (P30), 45 % (P45) and 60 % (P60) of the FM in the basal diet with PAM, respectively. The results showed that final body weight (FBW), weight gain rate (WGR), and specific growth rate (SGR) were no significant difference in P15 and P30 compared with the control group (P > 0.05). Crude protein of whole fish body was significantly increased and crude lipid was significantly decreased in all PAM groups (P < 0.05). In the P30, the antioxidant enzyme activities were significantly higher than those in the control and other experimental groups, and malondialdehyde (MDA) was significantly decreased (P < 0.05). The activities of α-amylase and pepsin increased significantly in P15 and P30 and decreased significantly in P45 and P60 (P < 0.05). Lipase was significantly increased in all groups except P15 than in the control group (P < 0.05). There were no significant differences in villus height (VH), crypt depth (CD), and muscle thickness (MT) among all groups (P > 0.05), but villus width (VW) was significantly decreased in P45 and P60 (P < 0.05). The relative expression of IL-10 and TGF-β1 were first up-regulated and then down-regulated, while IL-8 was opposite (P < 0.05). The relative expression of Casp3, Casp9 and IFN-γ were significantly up-regulated in P45 and P60 (P < 0.05). After injecting A. hydrophila, all groups of largemouth bass experienced mortality, but cumulative mortality was lowest in the P30. In conclusion, PAM can partially replace FM in the diet of juvenile largemouth bass. The replacement of no more than 30 % is unlikely to adversely affect growth performance, and can increase the crude protein content of the whole fish body, and is beneficial to intestinal health.http://www.sciencedirect.com/science/article/pii/S2352513424005337Periplaneta americana mealLargemouth bassGrowth performanceBody compositionIntestinal health
spellingShingle Yuanyi Liu
Changchang Pu
Zihui Wei
Yang Wang
Weichuan Zhang
Hongyu Chen
Yong Huang
Effects of dietary fish meal replacement by Periplaneta americana meal on growth, metabolism, intestinal health and resistance against Aeromonas hydrophila of Micropterus salmoides
Aquaculture Reports
Periplaneta americana meal
Largemouth bass
Growth performance
Body composition
Intestinal health
title Effects of dietary fish meal replacement by Periplaneta americana meal on growth, metabolism, intestinal health and resistance against Aeromonas hydrophila of Micropterus salmoides
title_full Effects of dietary fish meal replacement by Periplaneta americana meal on growth, metabolism, intestinal health and resistance against Aeromonas hydrophila of Micropterus salmoides
title_fullStr Effects of dietary fish meal replacement by Periplaneta americana meal on growth, metabolism, intestinal health and resistance against Aeromonas hydrophila of Micropterus salmoides
title_full_unstemmed Effects of dietary fish meal replacement by Periplaneta americana meal on growth, metabolism, intestinal health and resistance against Aeromonas hydrophila of Micropterus salmoides
title_short Effects of dietary fish meal replacement by Periplaneta americana meal on growth, metabolism, intestinal health and resistance against Aeromonas hydrophila of Micropterus salmoides
title_sort effects of dietary fish meal replacement by periplaneta americana meal on growth metabolism intestinal health and resistance against aeromonas hydrophila of micropterus salmoides
topic Periplaneta americana meal
Largemouth bass
Growth performance
Body composition
Intestinal health
url http://www.sciencedirect.com/science/article/pii/S2352513424005337
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