Isolation of Pancreatic Progenitor Cells with the Surface Marker of Hematopoietic Stem Cells
To isolate pancreatic progenitor cells with the surface markers of hematopoietic stem cells, the expression of stem cell antigen (Sca-1) and c-Kit and the coexpression of them with pancreatic duodenal homeobox-1 (PDX-1), neurogenin 3 (Ngn3), and insulin were examined in murine embryonic pancreas. Th...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2012/948683 |
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| author | Fengxia Ma Fang Chen Ying Chi Shaoguang Yang Shihong Lu Zhongchao Han |
| author_facet | Fengxia Ma Fang Chen Ying Chi Shaoguang Yang Shihong Lu Zhongchao Han |
| author_sort | Fengxia Ma |
| collection | DOAJ |
| description | To isolate pancreatic progenitor cells with the surface markers of hematopoietic stem cells, the expression of stem cell antigen (Sca-1) and c-Kit and the coexpression of them with pancreatic duodenal homeobox-1 (PDX-1), neurogenin 3 (Ngn3), and insulin were examined in murine embryonic pancreas. Then different pancreatic cell subpopulations were isolated by magnet-activated cell sorting. Isolated cells were cultured overnight in hanging drops. When cells formed spheres, they were laid on floating filters at the air/medium interface. With this new culture system, pancreatic progenitor cells were induced to differentiate to endocrine and exocrine cells. It was shown that c-Kit and Sca-1 were expressed differently in embryonic pancreas at 12.5, 15.5, and 17.5 days of gestation. The expression of c-Kit and Sca-1 was the highest at 15.5 days of gestation. c-Kit rather than Sca-1 coexpressed with PDX-1, Ngn3, and insulin. Cells differentiated from c-Kit-positive cells contained more insulin-producing cells and secreted more insulin in response to glucose stimulation than that from c-Kit-negative cells. These results suggested that c-Kit could be used to isolate pancreatic progenitor cells and our new culture system permitted pancreatic progenitor cells to differentiate to mature endocrine cells. |
| format | Article |
| id | doaj-art-6ab032de62694d9b9c6e7af90dbf64d4 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-6ab032de62694d9b9c6e7af90dbf64d42025-08-20T03:33:39ZengWileyInternational Journal of Endocrinology1687-83371687-83452012-01-01201210.1155/2012/948683948683Isolation of Pancreatic Progenitor Cells with the Surface Marker of Hematopoietic Stem CellsFengxia Ma0Fang Chen1Ying Chi2Shaoguang Yang3Shihong Lu4Zhongchao Han5State Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Disease, Chinese Academy of Medical Sciences, Tianjin 300020, ChinaState Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Disease, Chinese Academy of Medical Sciences, Tianjin 300020, ChinaState Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Disease, Chinese Academy of Medical Sciences, Tianjin 300020, ChinaState Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Disease, Chinese Academy of Medical Sciences, Tianjin 300020, ChinaState Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Disease, Chinese Academy of Medical Sciences, Tianjin 300020, ChinaState Key Laboratory of Experimental Hematology, Institute of Hematology and Hospital of Blood Disease, Chinese Academy of Medical Sciences, Tianjin 300020, ChinaTo isolate pancreatic progenitor cells with the surface markers of hematopoietic stem cells, the expression of stem cell antigen (Sca-1) and c-Kit and the coexpression of them with pancreatic duodenal homeobox-1 (PDX-1), neurogenin 3 (Ngn3), and insulin were examined in murine embryonic pancreas. Then different pancreatic cell subpopulations were isolated by magnet-activated cell sorting. Isolated cells were cultured overnight in hanging drops. When cells formed spheres, they were laid on floating filters at the air/medium interface. With this new culture system, pancreatic progenitor cells were induced to differentiate to endocrine and exocrine cells. It was shown that c-Kit and Sca-1 were expressed differently in embryonic pancreas at 12.5, 15.5, and 17.5 days of gestation. The expression of c-Kit and Sca-1 was the highest at 15.5 days of gestation. c-Kit rather than Sca-1 coexpressed with PDX-1, Ngn3, and insulin. Cells differentiated from c-Kit-positive cells contained more insulin-producing cells and secreted more insulin in response to glucose stimulation than that from c-Kit-negative cells. These results suggested that c-Kit could be used to isolate pancreatic progenitor cells and our new culture system permitted pancreatic progenitor cells to differentiate to mature endocrine cells.http://dx.doi.org/10.1155/2012/948683 |
| spellingShingle | Fengxia Ma Fang Chen Ying Chi Shaoguang Yang Shihong Lu Zhongchao Han Isolation of Pancreatic Progenitor Cells with the Surface Marker of Hematopoietic Stem Cells International Journal of Endocrinology |
| title | Isolation of Pancreatic Progenitor Cells with the Surface Marker of Hematopoietic Stem Cells |
| title_full | Isolation of Pancreatic Progenitor Cells with the Surface Marker of Hematopoietic Stem Cells |
| title_fullStr | Isolation of Pancreatic Progenitor Cells with the Surface Marker of Hematopoietic Stem Cells |
| title_full_unstemmed | Isolation of Pancreatic Progenitor Cells with the Surface Marker of Hematopoietic Stem Cells |
| title_short | Isolation of Pancreatic Progenitor Cells with the Surface Marker of Hematopoietic Stem Cells |
| title_sort | isolation of pancreatic progenitor cells with the surface marker of hematopoietic stem cells |
| url | http://dx.doi.org/10.1155/2012/948683 |
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