Diagnostic and Clinical Significance of Serum Levels of D-Lactate and Diamine Oxidase in Patients with Crohn’s Disease
Background. Crohn’s disease (CD) is a chronic intestinal inflammatory disease. An ideal laboratory marker that can predict the prognosis in terms of relapse of the disease is clinically desirable. Methods. A total of 59 CD patients were enrolled in this study. Enzyme-Linked Immunosorbent Assay (ELIS...
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| Format: | Article |
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Wiley
2019-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2019/8536952 |
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| author | Jierui Cai Hong Chen Meiling Weng Shuyu Jiang Jie Gao |
| author_facet | Jierui Cai Hong Chen Meiling Weng Shuyu Jiang Jie Gao |
| author_sort | Jierui Cai |
| collection | DOAJ |
| description | Background. Crohn’s disease (CD) is a chronic intestinal inflammatory disease. An ideal laboratory marker that can predict the prognosis in terms of relapse of the disease is clinically desirable. Methods. A total of 59 CD patients were enrolled in this study. Enzyme-Linked Immunosorbent Assay (ELISA) was used to quantitatively detect the content of D-lactate (D-LA) and the diamine oxidase (DAO) levels in sera obtained from patients and 28 healthy controls. The correlation between these two biomarkers and disease activity scores was assessed. In addition, the ROC curve was used to evaluate the diagnostic accuracy of these two biomarkers. Results. The levels of D-LA in the serum of CD patients in the active stage and remission stage were 16.08±4.8 mg/L and 11.16±3.17 mg/L, respectively, and the difference was statistically significant (t=4.67, P<0.001). DAO levels were significantly higher in patients with the active stage compared to controls. The levels of D-LA and DAO in CD patients were positively correlated with the disease activity (r=0.68 and 0.53, respectively, P<0.05). The area under the ROC curve (AUC) when CD activity was diagnosed with D-LA and DAO alone was 0.815 and 0.748, respectively. The diagnostic efficacy of the two biomarkers was not significantly different from that of the erythrocyte sedimentation (ESR) and hypersensitive C-reactive protein (CRP) (P>0.05). However, the area under the curve was 0.861 (0.746, 0.937) when the diagnosis was performed using a combination of D-LA, DAO, CRP, and ESR, which was significantly higher than when CRP or ESR were tested alone (P<0.05). Conclusions. D-LA and DAO have a good prognostic value for CD activity. Rational combined use of biomarkers can significantly improve the diagnostic efficiency. |
| format | Article |
| id | doaj-art-6aaf801bcdf74a129a2490cf73796c51 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-6aaf801bcdf74a129a2490cf73796c512025-02-03T06:01:47ZengWileyGastroenterology Research and Practice1687-61211687-630X2019-01-01201910.1155/2019/85369528536952Diagnostic and Clinical Significance of Serum Levels of D-Lactate and Diamine Oxidase in Patients with Crohn’s DiseaseJierui Cai0Hong Chen1Meiling Weng2Shuyu Jiang3Jie Gao4School of Medicine, Southeast University, Nanjing, ChinaSchool of Medicine, Southeast University, Nanjing, ChinaSchool of Medicine, Southeast University, Nanjing, ChinaSchool of Medicine, Southeast University, Nanjing, ChinaSchool of Medicine, Southeast University, Nanjing, ChinaBackground. Crohn’s disease (CD) is a chronic intestinal inflammatory disease. An ideal laboratory marker that can predict the prognosis in terms of relapse of the disease is clinically desirable. Methods. A total of 59 CD patients were enrolled in this study. Enzyme-Linked Immunosorbent Assay (ELISA) was used to quantitatively detect the content of D-lactate (D-LA) and the diamine oxidase (DAO) levels in sera obtained from patients and 28 healthy controls. The correlation between these two biomarkers and disease activity scores was assessed. In addition, the ROC curve was used to evaluate the diagnostic accuracy of these two biomarkers. Results. The levels of D-LA in the serum of CD patients in the active stage and remission stage were 16.08±4.8 mg/L and 11.16±3.17 mg/L, respectively, and the difference was statistically significant (t=4.67, P<0.001). DAO levels were significantly higher in patients with the active stage compared to controls. The levels of D-LA and DAO in CD patients were positively correlated with the disease activity (r=0.68 and 0.53, respectively, P<0.05). The area under the ROC curve (AUC) when CD activity was diagnosed with D-LA and DAO alone was 0.815 and 0.748, respectively. The diagnostic efficacy of the two biomarkers was not significantly different from that of the erythrocyte sedimentation (ESR) and hypersensitive C-reactive protein (CRP) (P>0.05). However, the area under the curve was 0.861 (0.746, 0.937) when the diagnosis was performed using a combination of D-LA, DAO, CRP, and ESR, which was significantly higher than when CRP or ESR were tested alone (P<0.05). Conclusions. D-LA and DAO have a good prognostic value for CD activity. Rational combined use of biomarkers can significantly improve the diagnostic efficiency.http://dx.doi.org/10.1155/2019/8536952 |
| spellingShingle | Jierui Cai Hong Chen Meiling Weng Shuyu Jiang Jie Gao Diagnostic and Clinical Significance of Serum Levels of D-Lactate and Diamine Oxidase in Patients with Crohn’s Disease Gastroenterology Research and Practice |
| title | Diagnostic and Clinical Significance of Serum Levels of D-Lactate and Diamine Oxidase in Patients with Crohn’s Disease |
| title_full | Diagnostic and Clinical Significance of Serum Levels of D-Lactate and Diamine Oxidase in Patients with Crohn’s Disease |
| title_fullStr | Diagnostic and Clinical Significance of Serum Levels of D-Lactate and Diamine Oxidase in Patients with Crohn’s Disease |
| title_full_unstemmed | Diagnostic and Clinical Significance of Serum Levels of D-Lactate and Diamine Oxidase in Patients with Crohn’s Disease |
| title_short | Diagnostic and Clinical Significance of Serum Levels of D-Lactate and Diamine Oxidase in Patients with Crohn’s Disease |
| title_sort | diagnostic and clinical significance of serum levels of d lactate and diamine oxidase in patients with crohn s disease |
| url | http://dx.doi.org/10.1155/2019/8536952 |
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