Baicalin-modified polyethylenimine for miR-34a efficient and safe delivery
The security and efficiency of gene delivery vectors are inseparable for the successful construction of a gene delivery vector. This work provides a practical method to construct a charge-regulated, hydrophobic-modified, and functionally modified polyethylenimine (PEI) with effective gene delivery a...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1290413/full |
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| author | Yingying Wang Yingying Wang Baiyan Wang Yangfan Xiao Qingchun Cai Junyue Xing Hao Tang Ruiqin Li Hongtao Zhang Hongtao Zhang Hongtao Zhang Hongtao Zhang |
| author_facet | Yingying Wang Yingying Wang Baiyan Wang Yangfan Xiao Qingchun Cai Junyue Xing Hao Tang Ruiqin Li Hongtao Zhang Hongtao Zhang Hongtao Zhang Hongtao Zhang |
| author_sort | Yingying Wang |
| collection | DOAJ |
| description | The security and efficiency of gene delivery vectors are inseparable for the successful construction of a gene delivery vector. This work provides a practical method to construct a charge-regulated, hydrophobic-modified, and functionally modified polyethylenimine (PEI) with effective gene delivery and perfect transfection performance through a condensation reaction, named BA-PEI. The carrier was shown to possess a favorable compaction of miRNAs into positively charged nanoparticles with a hydrodynamic size of approximately 100 nm. Additionally, BA-PEI possesses perfect degradability, which benefits the release of miR-34a from the complexes. In A549 cells, the expression level of the miR-34a gene was checked by Western blotting, which reflects the transfection efficiency of BA-PEI/miR-34a. When miR-34a is delivered to the cell, the perfect anti-tumor ability of the BA-PEI/miR-34a complex was systematically evaluated with the suppressor tumor gene miR-34a system in vitro and in vivo. BA-PEI-mediated miR-34a gene transfection is more secure and effective than the commercial transfection reagent, thus providing a novel approach for miR-34a-based gene therapy. |
| format | Article |
| id | doaj-art-6aaf4e0958cb4e4a81d71beb060b4f7b |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-6aaf4e0958cb4e4a81d71beb060b4f7b2025-08-20T03:50:44ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-11-011110.3389/fbioe.2023.12904131290413Baicalin-modified polyethylenimine for miR-34a efficient and safe deliveryYingying Wang0Yingying Wang1Baiyan Wang2Yangfan Xiao3Qingchun Cai4Junyue Xing5Hao Tang6Ruiqin Li7Hongtao Zhang8Hongtao Zhang9Hongtao Zhang10Hongtao Zhang11Medical College, Henan University of Chinese Medicine, Zhengzhou, Henan, ChinaNational Health Commission Key Laboratory of Cardiovascular Regenerative Medicine, Heart Center of Henan Provincial People’s Hospital, Fuwai Central China Cardiovascular Hospital and Central China Branch of National Center for Cardiovascular Diseases, Central China Fuwai Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaMedical College, Henan University of Chinese Medicine, Zhengzhou, Henan, ChinaNational Health Commission Key Laboratory of Cardiovascular Regenerative Medicine, Heart Center of Henan Provincial People’s Hospital, Fuwai Central China Cardiovascular Hospital and Central China Branch of National Center for Cardiovascular Diseases, Central China Fuwai Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Clinical Lab, The Third Affiliated Hospital of Henan University of Chinese Medicine, Zhengzhou, ChinaNational Health Commission Key Laboratory of Cardiovascular Regenerative Medicine, Heart Center of Henan Provincial People’s Hospital, Fuwai Central China Cardiovascular Hospital and Central China Branch of National Center for Cardiovascular Diseases, Central China Fuwai Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaNational Health Commission Key Laboratory of Cardiovascular Regenerative Medicine, Heart Center of Henan Provincial People’s Hospital, Fuwai Central China Cardiovascular Hospital and Central China Branch of National Center for Cardiovascular Diseases, Central China Fuwai Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaAcademy of Chinese Medicine, Henan University of Chinese Medicine, Zhengzhou, Henan, ChinaBlood Purification Center, The People’s Hospital of Zhengzhou University, Zhengzhou, ChinaBlood Purification Center, Henan Provincial People’s Hospital, Zhengzhou, ChinaInstitute of Nephrology, Mathura, Henan, ChinaDepartment of Nephrology Henan Provincial People’s Hospital, Zhengzhou, ChinaThe security and efficiency of gene delivery vectors are inseparable for the successful construction of a gene delivery vector. This work provides a practical method to construct a charge-regulated, hydrophobic-modified, and functionally modified polyethylenimine (PEI) with effective gene delivery and perfect transfection performance through a condensation reaction, named BA-PEI. The carrier was shown to possess a favorable compaction of miRNAs into positively charged nanoparticles with a hydrodynamic size of approximately 100 nm. Additionally, BA-PEI possesses perfect degradability, which benefits the release of miR-34a from the complexes. In A549 cells, the expression level of the miR-34a gene was checked by Western blotting, which reflects the transfection efficiency of BA-PEI/miR-34a. When miR-34a is delivered to the cell, the perfect anti-tumor ability of the BA-PEI/miR-34a complex was systematically evaluated with the suppressor tumor gene miR-34a system in vitro and in vivo. BA-PEI-mediated miR-34a gene transfection is more secure and effective than the commercial transfection reagent, thus providing a novel approach for miR-34a-based gene therapy.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1290413/fullbaicalinlung cancergene therapymiR-34ahydrophobic modification |
| spellingShingle | Yingying Wang Yingying Wang Baiyan Wang Yangfan Xiao Qingchun Cai Junyue Xing Hao Tang Ruiqin Li Hongtao Zhang Hongtao Zhang Hongtao Zhang Hongtao Zhang Baicalin-modified polyethylenimine for miR-34a efficient and safe delivery Frontiers in Bioengineering and Biotechnology baicalin lung cancer gene therapy miR-34a hydrophobic modification |
| title | Baicalin-modified polyethylenimine for miR-34a efficient and safe delivery |
| title_full | Baicalin-modified polyethylenimine for miR-34a efficient and safe delivery |
| title_fullStr | Baicalin-modified polyethylenimine for miR-34a efficient and safe delivery |
| title_full_unstemmed | Baicalin-modified polyethylenimine for miR-34a efficient and safe delivery |
| title_short | Baicalin-modified polyethylenimine for miR-34a efficient and safe delivery |
| title_sort | baicalin modified polyethylenimine for mir 34a efficient and safe delivery |
| topic | baicalin lung cancer gene therapy miR-34a hydrophobic modification |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1290413/full |
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